Evaluation of Cytotoxicity and Genotoxicity of Insecticide Carbaryl to Flounder Gill Cells and Its Teratogenicity to Zebrafish Embryos

In this study, we determined the cytotoxicity and genotoxicity of carbamate insecticide carbaryl to flounder gill(FG) cells and its teratogenicity to zebrafish embryos. The cytotoxicity of carbaryl to FG cells was determined with methods including MTT and neutral red uptaking(NRU), lactate dehydrogenase(LDH) releasing and Hoechst 33342 and propidium idodide(PI) double staining. Moderate cytotoxicity in a concentration-dependent manner was observed. The 24 h-IC50 value of 53.48 ± 1.21, 59.13 ± 1.19 and 46.21 ± 1.24 mg L-1 carbaryl was obtained through MTT, NRU and LDH assays, respectively. Double fluorescence staining demonstrated that carbaryl induced the death of FG cells mainly through necrosis. There was no significant genotoxicity found in the FG cells exposed to the highest testing concentration of carbaryl(20 mg L-1, P 0.05) as was demonstrated by Comet assay. Zebrafish embryos exposed to carbaryl at concentrations ≥10 mg L-1 displayed moderate toxic effects on the survival, spontaneous movement, hatching, heart rates of the embryos and their development, which were evidenced by yolk and pericardial sac edemas, body length reduction and tail flexure in time- and concentration-dependent manners at specific stages. The 24 h-, 48 h- and 96 h-LC50 values of carbaryl to zebrafish embryos were 41.80 ± 1.10, 17.80 ± 1.04 and 14.46 ± 1.05 mg L-1, respectively. These results suggested that carbaryl is moderately toxic to FG cells cultured in vitro and zebrafish embryos, and the FG cells were similar to zebrafish embryos in their sensitivity to carbaryl as 24 h-IC50 and LC50 indicated.     

南堡凹陷东营组高精度层序地层格架及沉积体系分析

在高分辨率三维地震、钻井以及大量地质资料研究基础上,根据层序地层界面特征,将南堡凹陷东营组划分为1个二级层序和4个三级层序。以初始洪泛面和最大洪泛面识别特征为依据,进一步将三级层序划分出体系域。通过层序地层格架特征及其发育位置分析,认为柳南次凹层序地层格架发育的主控因素为构造、物源和湖平面变化。应用层序地层学原理及南堡凹陷沉积充填背景研究,认为柳南次凹的沉积体系类型为冲积扇、扇三角洲、水下扇、重力流及湖泊沉积体系。冲积扇及水下扇体系在低位体系域中发育,高位体系域中扇三角洲体系和重力流发育,湖扩体系域中湖泊体系非常发育。     

滇东地区煤田构造格局与煤系赋存规律的研究

滇东地区位于特提斯和环太平洋构造体系的交接部位,煤田构造格局的总体特征是呈NNE向条带状展布。区内断层以NNE、NE向为主,受主要断裂影响,煤田在南北向呈带状,东西向呈段状展布,各煤矿区在平面上呈NE向带状分布。研究区煤系赋存受基底主干断裂的控制作用明显,变形强度由北向南逐渐增强,北部地区以褶皱构造为主,断层较少,煤层多保存于向斜构造中,保存较完整,煤层埋藏较深;南部受弥勒-师宗断裂带影响,断裂构造极为发育,对煤层破坏严重,煤系赋存不稳定。     

兰州地区最古老黄土剖面的粒度学及孢粉学研究

兰州地区最古老黄土剖面露头良好,地层出露较齐全,通过剖面磁性地层研究,确定兰州地区最古老黄土形成于1.84MaBP以前,对山城湾岭黄土剖面进行了粒度及孢粉的研究,探讨了第四纪沉积环境的演化。     

共反射元弧叠加方法在火山岩成像中的应用

常规叠加方法假设叠加的地震道集的反射波来自地下同一个反射点,而地表观测到 的反射波场在空间上小于菲涅尔体范围是不可分辨的. 本文根据火山岩体特征,用元弧描述 火山岩的反射界面. 将来自菲涅尔体范围内的反射波沿着元弧界面求和,进而扩大叠加次数 ,达到增加反射信号能量提高地震反射波的叠加成像质量的目的. 文中给出了反射波走时参 数与地下元弧反射界面之间的联系和叠加实现的步骤. 计算结果表明,元弧叠加方法能够提 高剖面的信噪比,增加反射波同相轴的连续性.     

华夏植物群起源中心的研究

要贺兰山一带石炭纪、二叠纪地层发育,晚石炭世早期至二叠纪的地层连续出露,其中富含植物化石,特别是晚石炭世纳缪尔B—C期的红土洼组含丰富的早期华夏植物群分子。笔者多年来的研究表明,在此连续剖面上,植物化石反映出植物群的演化趋势颇为明显,构成石炭纪和二叠纪植物群较为连续的演化序列。植物群中含有大量的华夏型分子,属典型的华夏植物群。内蒙古阿拉善左旗呼鲁斯太剖面晚石炭世早期红土洼组是迄今为止含华夏植物群的最有代表性层位,所含植物化石是目前已记载的最为丰富的晚石炭世早期华夏型植物组合,因此笔者认为贺兰山一带可视为华夏植物群的起源中心。     

The Classification and Model of Coalbed Methane Reservoirs

Coalbed methane has been explored in many basins worldwide for 30 years, and has been developed commercially in some of the basins. Many researchers have described the characteristics of coalbed methane geology and technology systematically. According to these investigations, a coalbed methane reservoir can be defined: "a coal seam that contains some coalbed methane and is isolated from other fluid units is called a coalbed methane reservoir". On the basis of anatomization, analysis, and comparison of the typical coalbed methane reservoirs, coalbed methane reservoirs can be divided into two classes: the hydrodynamic sealing coalbed methane reservoirs and the self-sealing coalbed methane reservoirs. The former can be further divided into two sub-classes: the hydrodynamic capping coalbed methane reservoirs, which can be divided into five types and the hydrodynamic driving coalbed methane reservoirs, which can be divided into three types. The latter can be divided into three types. Currently, hydrodynamic s     

中国铜矿床品位吨位模型

笔者等以1999年底的全国铜矿储量数据为基础,研究了中国铜矿不同成矿类型的品位吨位分布特征,在此基础上研究了中国斑岩型铜矿的品位、吨位分形特征。在研究铜矿的品位吨位特征的基础上,在矿产资源评价系统(MRAS)上集成MRAK3计算方式,进行全国的铜矿资源量预测评价。     

Distribution Characteristics of Effective Source Rocks and Their Control on Hydrocarbon Accumulation: A Case Study from the Dongying Sag, Eastern China

The exploration conducted in the Bohai Bay basin, eastern China has demonstrated that the abundant petroleum resources have close affinities to the hydrocarbon kitchen with rich organic matter. A number of oil-generating associations with various characteristics of organic geochemistry and assemblages of multiple reservoir facies are developed due to the multi-center sedimentation, multi-source supply and multi-cycle evolution of filling, which have resulted in the formation of multiple oil and gas accumulation zones of various layers and trap styles. Among them the Paleogene Shahejie Formation is the most important hydrocarbon accumulation combination in the Dongying sag. Heretofore, its proved reserve has reached nearly 1.8×109t, which accounts for more than 90% of the total proved reserves of the Dongying sag. Based on previous studies, more than 600 source rock samples and 186 crude oil samples of the Shahejie Formation, collected from 30 oilfields, have been treated with organic geochemical testing     

塔里木盆地肖尔布拉克剖面下丘里塔格组层序地层及其油气勘探意义

塔里木盆地晚寒武世台地沉积区发育大量的白云岩,其中蕴涵着大量白云岩储层。以柯坪地区肖尔布拉克剖面为例,首次提出了以地层单层厚度为划分依据划分层序地层的方法,结合岩石学分析结果,确定了肖尔布拉克剖面寒武系下丘里塔格组白云岩的层序地层四分方案,塔中地震资料表明该四分方案可能适用于塔里木盆地整个西部台地区。根据肖尔布拉克剖面下丘里塔格组白云岩的岩石学分析,埋藏岩溶作用产生的溶蚀空间为该白云岩段的主要储集空间,因此可将下丘里塔格组白云岩储层定义为"埋藏岩溶型白云岩储层",该储层发育位置与层序界面有密切关系,一般沿层序界面之下发育。根据上寒武统白云岩的层序地层四分方案,与层序地层对储层的控制作用,提出了塔里木盆地西部台地区上寒武统"埋藏岩溶型复式白云岩储层"概念,并给予了阐释。