Diel Patterns in Chlorophyll <Emphasis Type="Italic">a</Emphasis> Specific Absorption Coefficient and Absorption Efficiency Factor of Picoplankton

Diel patterns in the chlorophyll a specific absorption coefficient of surface picoplankton, a*_pico (γ) (m²·[mg chlorophyll a]⁻¹), were studied at 7 stations under daily cycle of in situ light condition in the western subarctic Pacific and Japan Sea. All the data were normalized by dividing the anomaly with daily averaged a*_pico (γ). Opposite diel patterns were observed for the normalized a*_pico (443) and a*_pico (675) with maximum toward dawn or dusk and minimum toward midday at 4 stations under low-irradiance (LI) conditions and vice versa at 3 stations under high-irradiance (HI) conditions. The absorption efficiency factors at red absorption peak, Q _a (675), were determined by reconstruction with intracellular chlorophyll a concentration and cell diameter. The normalized Q _a (675) also showed diel pattern with maximum toward midday and minimum toward dawn or dusk under LI. The diel pattern in a*_pico (675) and Q _a (675) were primarily caused by changes in intracellular chlorophyll a concentration due to photoadaptation under LI. The diel pattern in a*_pico (443) was influenced by pigmentation, as recognized by blue to red ratio [a*_pico (443)/a*_pico (675)] under HI. This study proposed that the opposite diel pattern in a*_pico (γ) might occur for a wide range of algal species. The results presented here have important consequences for the interpretation of diel variations in optical properties observed in the open ocean.     

江苏大丰潮滩潮流边界层特征研究

2003年7月中小潮期间使用MIDAS-400用户化数据采集系统在江苏大丰潮滩上进行了垂向多层位同步的流速和悬沙浓度观测。根据观测资料研究了潮流作用下的潮滩底部边界层过程,结果表明,观测地点的潮汐不对称现象十分明显,落潮流速、历时和输水输沙量皆明显大于涨潮;在潮周期内的多数时间里流速剖面符合对数分布,但在风力较强、水流快速增加和流速缓慢的情况下,流速剖面常偏离对数分布;悬沙颗粒垂向混合均匀,悬沙浓度剖面符合Rouse公式;落潮时的底部切应力和摩阻流速明显大于涨潮,摩阻流速与各水层流速通常有较好的线性关系;滩面糙度主要与沙纹形态和推移质运动强度有关,它与摩阻流速存在密切关系;悬沙浓度具有明显的减阻效应,受其影响,摩阻流速和底部切应力分别减小了28%~41%和40%~62%。     

用包埋脱水法冰冻保存海洋饵料金藻

用包埋脱水法冰冻保存绿色巴夫藻(Pavlova uiridis)、湛江等鞭金藻(Isochrysis zhanjiangensis)和球等鞭金藻(Isochrysis galbana 3011)等三种海洋饵料金藻，探讨了脱水速率、胶球含水量以及化冻后恢复方法对冰冻保存存活率的影响。结果表明，三种藻都在-0．9％含水量／h的平均脱水速率下获得最高存活率：各种藻在冰冻前的胶球最佳含水量不同，绿色巴夫藻为35％，湛江等鞭金藻和球等鞭金藻都为30％。化冻后，含绿色巴夫藻的胶球在培养基中22℃暗放置48h存活率最高；另两种藻在相对湿度为75％的气相中22℃暗放置12h存活率最高。在本实验条件下，绿色巴夫藻、湛江等鞭金藻和球等鞭金藻的冰冻保存的存活率可分别达到74％、15％和17％。     

1999年渤海浮游植物生物量的数值模拟

以浮游植物量、浮游动物量、营养盐浓度 (包括无机氮和无机磷 )以及碎屑量为生态变量 ,在HAMSOM水动力学模式的基础上构建了 1个三维浮游生态动力学NPZD模型。采用此模型研究了渤海 1999年浮游植物量和初级生产力的变化情况 ,模拟结果与实测基本相符。模拟结果表明 :1999年渤海浮游植物量的变化大致呈双峰分布 ,春季水华出现在4,5月份 ,秋季水华出现在 9,10月份 ;受透明度和局地水深的影响 ,渤海湾和辽东湾北部浮游植物量的年变化呈夏季大、冬季小的单峰分布。 1999年渤海不同海区初级生产力的变化特征是 :除莱州湾一年中有春、夏 2个峰值外 ,其它 3个海区都是夏季高、冬季低的单峰分布 ;1999年整个渤海年平均的初级生产力为 2 5 7mgC/m2 /d。     

山东近海牙鲆(Paralichthys olivaceus)自然和养殖群体10个微卫星基因座位的遗传多态性分析

采用微卫星遗传标记技术对山东近海牙鲆自然群体和养殖群体的遗传多样性进行了研究。实验所用牙鲆于2003年5月分别采自山东近海和青岛胶南养鱼场，各20尾，取全血或肌肉组织，以酚-仿抽提方法提取基因组DNA，利用筛选获得的10对微卫星引物进行PCR扩增，反应产物经8％非变性聚丙烯酰胺凝胶电泳分离、EB显色，用ImageMasterl D Elite(Version 3．01)软件分析电泳结果，并计算了相应的遗传学参数。结果表明，在自然和养殖群体中，10个基因座位的等位基因平均数(α)分别为6．7和6．1，每个基因座位有效等位基因数(αe)分别为1．8—6．8和2．5—6．7，群体平均杂合度(H)分别为0．8120和0．7310；两个群体间的遗传相似性系数、遗传距离和基因分化系数为0．8558、0．1557和0．0558；自然群体内每个座位上的多态信息含量(PIC)为0．59—0．84、个体识别率(DP)为0．54—0．86、非父排除率(PPE)为0．41—0．72，其累积个体识别率和非父排除率均达到0．9999，表明所选座位属中高识别力的遗传标记，可以将它们应用于今后牙鲆雌核发育群体的遗传变异分析以及进一步的遗传育种的研究中。     

CO<Subscript>2</Subscript> Flux Measurements over the Sea Surface by Eddy Correlation and Aerodynamic Techniques

The measurements of the vertical transport of CO₂ were carried out over the Sea of Japan using the specially designed pier of Kyoto University on September 20 to 22, 2000. CO₂ fluxes were measured by the eddy correlation and aerodynamic techniques. Both techniques showed comparable CO₂ fluxes during sea breeze conditions: −0.001 to −0.08 mg m⁻²s⁻¹ with the mean of −0.05 mg m⁻²s⁻¹. This means that the measuring site satisfies the fetch requirement for meteorological observations under sea breeze conditions. Moreover, the eddy diffusivity coefficient used in the aerodynamic technique is found to be consistent with the coefficient used in the eddy correlation technique. The present result leads us to conclude that the aerodynamic technique may be applicable to underway CO₂ flux measurements over the ocean and may be used in place of the bulk technique. The important point is the need to maintain a measuring accuracy of CO₂ concentration difference of the order of 0.1 ppmv on the research vessels or the buoys.     

Roles of Biogeochemical Processes in the Oceanic Carbon Cycle Described with a Simple Coupled Physical-Biogeochemical Model

To evaluate the contribution of biogeochemical processes to the oceanic carbon cycle and to calculate the ratio of calcium carbonate to organic carbon downward export, we have incorporated biological and alkalinity pumps in the yoked high-latitude exchange/interior diffusion-advection (YOLDA) model. The biogeochemical processes are represented by four parameters. The values of the parameters are tuned so that the model can reproduce the observed phosphate and alkalinity distributions in each oceanic region. The sensitivity of the model to the biogeochemical parameters shows that biological production rates in the euphotic zone and decomposition depths of particulate matters significantly influence horizontal and vertical distributions of biogeochemical substances. The modeled vertical fluxes of particulate organic phosphorus and calcium carbonate are converted to vertical carbon fluxes by the biological pump and the alkalinity pump, respectively. The downward carbon flux from the surface layer to the deep layer in the entire region is estimated to be 3.36 PgC/yr, which consists of 2.93 PgC/yr from the biological pump and 0.43 PgC/yr from the alkalinity pump, which is consistent with previous studies. The modeled rain ratio is higher with depth and higher in the Pacific and Indian Oceans than in the Atlantic Ocean. The global rain ratio at the surface layer is calculated to be 0.14 to 0.15. This value lies between the lower and higher ends of the previous estimates, which range widely from 0.05 to 0.25. This study indicates that the rain ratio is unlikely to be higher than 0.15, at least in the surface waters.     

日本(虫寻)和底栖短桨蟹线粒体DNA 12SrRNA基因序列的初步研究

以相应引物对日本和底栖短桨蟹的线粒体 DNA1 2 S r RNA基因片段进行了 PCR扩增和序列测定 ,分析比较了 2种间序列差异。结果表明 :日本 1 2 S r RNA基因片段长度为 41 4 bp,底栖短桨蟹为 41 5bp,2种的 A,T,G,C含量分别为 1 51 bp(36.47% ) ,1 53bp(36.96% ) ,43bp(1 0 .39% ) ,67bp(1 6.1 8% )和 1 50 bp(36.1 4 % ) ,1 55bp(37.35% ) ,44bp(1 0 .60 % ) ,66bp(1 5.90 % )。 2种间共出现了 3个碱基的缺失 /插入和 38bp的序列差异 ,其碱基转换与碱基颠换比约为 2 .1 7。     

Characteristics of Scour and Deposition in Front of Breakwaters Under Irregular Broken Clapotis

First the scour and deposition patterns of the sandy seabed in front of a vertical breakwater under the action of irregular broken clapotis are investigated experimentally and classified into five types: scour type Ⅰ , scour type Ⅱ, scour type Ⅲ, deposition type Ⅰ, and deposition type Ⅱ . Secondly, the processes of formation of scour and deposition patterns are probed in comparison with those induced by regular broken clapotis and standing waves. Thirdly, the criteria for distinguishing scour and deposition patterns under irregular broken clapotis are presented.     

Long-Term Investigation of Spatio-Temporal Variations in Faunal Composition and Species Richness of Megabenthos in Ise Bay,Central Japan

Based on our long-term data from megabenthos sampling from 1993 to 2002 in Ise Bay, central Japan, we examined spatio-temporal variations in taxon composition, species richness and its distribution of megabenthos in the bay in relation to the occurrence of the oxygen-poor water (i.e. oxygen content less than 3 ppm) in bottom waters of the bay. A total of 261 species were identified including 6 cnidarians, 1 tentaculate, 5 annelids, 71 molluscs, 72 crustaceans, 16 echinoderms, 12 urochordates and 78 pisces. Of the most abundant 10 megabenthos species, the following 4 species of echinoderms made up more than the half of megabenthos biomass: Luidia quinaria, Echinocardium cordatum, Asterias amurensis and Astropecten scoparius. Species richness of megabenthos varied significantly between seasons and among stations. The severity and period of occurrence of the oxygen-poor water developing every summer play an important role in determining spatial distributions of species richness in the bay.