由偏顶蛤贝壳中稳定同位素剖面追溯海水温度记录

本文用在加拿大诺瓦·斯科舍省芬克湾采集到的两只活体偏顶蛤(Modiolus)贝壳上的氧同位素组成重建了过去十年间的底层海水温度记录,它也可作为一种独立的方法来确定贝壳上外生长线的沉积时间。由于对贝壳进行了密集采样(可多至每个贝壳沉积年份取15个样品),贝壳上的δ~(16)O变化反映了芬克湾底层海水的季节性变化。两只贝壳所得的温度剖面符合得很好,说明它们基本上是与真实的环境温度相对应的。本研究证明在海胆疾病爆发时底层海水的温度并没有异常升高,这说明疾病传播的关键因素是带病的生物体,而不是温度。同位素技术也说明了在贝壳外表面上可见的外生长线是年轮性的,它们是在每年冬季最低水温时沉积出来的。     

Population structure of Haliotis rubra from South Australia inferred from nuclear and mtDNA analyses

1Introduction ThemajorityofAustralia’sabalonefisheryex ports(5.135kt,worth＄216millionin2002～2003,ABARE2004)consistofblacklipabalone(HaliotisrubraLeach,1814).AssuchH.rubrais consideredasanimportantmarineresourcewithin Australia.Likemanyabalonespecieswor…     

Bacterial killing by Mytilus hemocyte monolayers as a model for investigating the signaling pathways involved in mussel immune defence

The signaling pathways involved in mussel immune defence were investigated utilizing a model of killing of Escherichia coli by Mytilus galloprovincialis hemocytes in a co-culture setting. In particular, the role played by different mitogen activated protein kinases (MAPKs) and by the production of eicosanoids were investigated utilising specific cell permeant, pharmacological enzyme inhibitors. Hemocyte pretreatment with the p38 MAPK inhibitor SB203580 significantly reduced bacterial killing, whereas PD98059 (an inhibitor of ERK--extracellularly regulated kinase--MAPK activation) had no significant effect. Wortmannin also inhibited bacterial killing, indicating a crucial role for PI3-kinase activation in the immune response. Killing of E. coli was also reduced by inhibitors of both PLA2 and cyclooxygenase activities, indicating that eicosanoid production is involved in mediating the response to bacterial challenge. The results demonstrate that bacterial killing by mussel hemocytes is particularly sensitive to inhibitors of the key steps involved in the transduction of bacterial signals into the host cell. Moreover, these data indicate that the hemocyte bactericidal activity can be suitably utilized not only for identifying the signaling pathways involved in the response to bacterial infection, but also as a potential investigative-toxicology model to test drugs and contaminants for their effect on the overall mussel immune defence.     

Can turbidity caused by Corophium volutator (Pallas) activity be used to assess sediment toxicity rapidly?

The standard toxicity test organism, Corophium volutator, exhibits a behavioural response to contaminated sediments that causes increased turbidity of overlying water. We quantify the effects of this response to an estuarine sediment spiked with copper and hydrocarbon contaminated sediments from an oil installation in the North Sea. Turbidity measured 24 h after the start of a toxicity test shows a strong relationship with contaminant concentrations and with mortality after 10 days. Turbidity measurements can therefore give a rapid indication of sediment toxicity, permitting a reduction in storage time of sediments to be used in dilution series and toxicity identification evaluation (TIE) tests, reducing the likelihood of contaminants degrading prior to testing.     

五种植物提取液的抗鱼病菌和抗鱼病毒效应(英文)

本文旨在探索用廉价的野生植物防治鱼病。所用的植物有牛繁缕(Stellaria aqu-atica),双花凤仙(Impatiens biflora),夜来香(Oenothera biennis),艾草(Artem-isia vulgaris)和忍冬(Lonicera japonica)。用作试验的鱼病菌共13种(18个菌株),分属于气单孢菌属、假单孢菌属、爱德华氏菌属、耶尔森氏菌属、弧菌属。所用的鱼病毒为传染性胰脏坏死病毒IPNV,传染性造血组织坏死病毒IHNV。上述病原微生物均获自美国马里兰大学微生物系。实验结果表明,五种植物提取液均有不同程度的抗鱼病菌作用,其中以牛繁缕的抗菌谱最广,作用力最强。忍冬、艾草、牛繁缕有抗鱼病毒作用。其中以忍冬的效果最好,它能抑制IPNV和IHNV。而艾草和牛繁缕只能抑制IHNV。     

Environmental consequence analyses of fish farm emissions related to different scales and exemplified by data from the Baltic--a review

The aim of this work is to review studies to evaluate how emissions from fish cage farms cause eutrophication effects in marine environments. The focus is on four different scales: (i) the conditions at the site of the farm, (ii) the local scale related to the coastal area where the farm is situated, (iii) the regional scale encompassing many coastal areas and (iv) the international scale including several regional coastal areas. The aim is to evaluate the role of nutrient emissions from fish farms in a general way, but all selected examples come from the Baltic Sea. An important part of this evaluation concerns the method to define the boundaries of a given coastal area. If this is done arbitrarily, one would obtain arbitrary results in the environmental consequence analysis. In this work, the boundary lines between the coast and the sea are drawn using GIS methods (geographical information systems) according to the topographical bottleneck method, which opens a way to determine many fundamental characteristics in the context of mass balance calculations. In mass balance modelling, the fluxes from the fish farm should be compared to other fluxes to, within and from coastal areas. Results collected in this study show that: (1) at the smallest scale (<1 ha), the "footprint" expressing the impact areas of fish cage farm often corresponds to the size of a "football field" (50-100 m) if the annual fish production is about 50 ton, (2) at the local scale (1 ha to 100 km2), there exists a simple load diagram (effect-load-sensitivity) to relate the environmental response and effects from a specific load from a fish cage farm. This makes it possible to obtain a first estimate of the maximum allowable fish production in a specific coastal area, (3) at the regional scale (100-10,000 km2), it is possible to create negative nutrient fluxes, i.e., use fish farming as a method to reduce the nutrient loading to the sea. The breaking point is to use more than about 1.1 g wet weight regionally caught wild fish per gram feed for the cultivated fish, and (4) at the international scale (>10,000 km2) related to the Baltic Proper, the contribution from fish farms to the overall nutrient fluxes are very small. We have also given two case-studies at the local scale where the impact of the fish farm emissions are greatest and the idea is to identify coastal areas unsuitable and suitable for fish cage farms and the reasons why. It should also be stressed that the results presented here are exemplified using emissions from fish farms, but that the underlying principles to evaluate the ecosystem effects of nutrient discharges from point source emissions are valid in a wider and more general perspective.     

1nm锰矿相的金属阳离子交换能力的研究

人工合成的1nm锰矿相和铜、钴、镍等二价金属阳离子的交换实验表明,1nm锰矿相具有强烈的金属阳离子交换能力.正是这种金属阳离子的交换能力,使得大洋多金属结核大量地富集了铜、钴、镍等有价金属元素.1nm锰矿相对几种金属阳离子的交换的优先顺序为:Cu>Co>Zn≥Ni>Ca>Mg.多金属结核中镍的含量往往高于铜、钴、锌等元素,但在交换顺序中它却低于铜、钴、锌等,这种不一致性说明结核成矿后期的金属离子交换并非是有价金属结合进结核的唯一途径.多金属结核富含铜、钴、镍等有价金属可能是结核初始成矿和成矿后期金属离子交换等多种因素共同作用的结果.