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Flatfish or flounder moves one eye to change body proportion into vertebral asymmetry during metamorphosis, during which some
become sinistral while others dextral. However, the mechanism behinds the eye-position has not been well understood. In this
research, hybrids between Japanese flounder(♀) and stone flounder (♂) show mixed eye-location in both dextral type and sinistral
type, and thus become good samples for studying the eye-migration. mRNAs from pro-metamorphosis sinistral and dextral hybrids
larvae were screened with classical differential display RT-PCR (DD-RT-PCR) and representational difference analysis of cDNA
(cDNA-RDA); 30 and 47 putative fragments were isolated, respectively. The cDNA fragments of creatine kinase and trypsinogen
2 precursor genes isolated by cDNA-RDA exhibited eye-position expression patterns during metamorphosis. However, none of the
fragments was proved to be related to flatfishes’ eye-position specifically. Therefore, further studies and more sensitive
gene isolated methods are needed to solve the problems.
Supported by National Natural Science Foundation of China (No. 30600455) and the National Basic Research Program of China
(973 Program, No.2004CB117402) 相似文献
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我区农田、草地土壤墒情类型及其相对含水率等级指标的确定 总被引:1,自引:0,他引:1
作为定性评定土壤湿度的方法,即以土壤墒情类型分析、制作的服务产品已在气象业务中得到广泛使用。本文参照一些学者有关这方面的研究成果和有关规定,并在多年经验积累基础上,根据我区自然地理条件、气候条件与农作物或牧草生长发育需水特征之间的关系,分别给出我区农田、草地的5 种不同土壤墒情类型和土深0~20cm 的相对含水率等级指标。近年来通过全区土壤墒情分析产品的应用效果反馈,表明所确定结果可行。 相似文献
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利用深圳2003-2005年18个自动站气温资料,选取坪山、梧桐村和石岩测场的3个自动测站作为热岛对比分析站,分析深圳近三年来深圳年平均热岛强度的空间分布及季节、月际间变化特征,并通过相应MODIS资料进行实时验证;为预估热岛情景,使用1961-2006年深圳、台山、遮浪三站的气候资料,建立深圳热岛时间-强度拟合方程,预估2020年深圳市热岛强度为0.8-0.9℃。 相似文献
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The symmetrical body of flatfish larvae changes dramatically into an asymmetrical form after metamorphosis. The molecular mechanisms responsible for this change are poorly understood. As an initial step to clarify these mechanisms, we used representational difference analysis of cDNA for the identification of genes active during metamorphosis in the Japanese flounder, Paralichthys olicaceus. One of the up-regulated genes was identified as creatine kinase muscle type 1 (CK-M1). Sequence analysis of CK-M1 revealed that it spanned 1 708 bp and encoded a protein of 382 amino acids. The overall amino acid sequence of the CK-M1 was highly conserved with those of other organisms. CK-M1 was expressed in adult fish tissues, including skeletal muscle, intestine and gill. Whole mount in-situ hybridization showed that the enhanced expression of CK-M1 expanded from the head to the whole body of larvae as metamorphosis progressed. Quantitative analysis revealed stage-specific high expression of CK-M1 during metamorphosis. The expression level of CK-M1 increased initially and peaked at metamorphosis, decreased afterward, and finally returned to the pre-metamorphosis level. This stage-specific expression pattern suggested strongly that CK-M1 was related to metamorphosis in the Japanese flounder. Its specific role in metamorphosis requires further study. 相似文献
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对贵州省麦岗水库沉积物环境磁性特征的研究表明,亚铁磁性矿物主导了沉积物矿物磁性特征,但同时也存在反铁磁性矿物等其他矿物,超顺磁颗粒在沉积物中广泛存在.在所选矿物磁性参数中,χlf、χfd、SOFT与粒度不相关;χARM、SIRM、F300与粒度相关,但相关系数不高;χARM/χlf、χARM/SIRM和粒度显著相关,可以作为粒度的代用指标.研究结果显示,磁性参数确实可以作为粒度的代用指标.但对比研究表明,在不同沉积环境,甚至相似沉积环境的沉积物中,矿物磁性参数和粒度的关系可能不同,在特定沉积环境中,利用磁性参数作为粒度的代用指标应该在充分研究的基础上进行,使研究结果更为可靠. 相似文献