Use of suppressive subtractive hybridization and cDNA arrays to discover patterns of altered gene expression in the liver of dihydrotestosterone and 11-ketotestosterone exposed adult male largemouth bass (Micropterus salmoides)

In this study male largemouth bass (LMB) were exposed to the naturally occurring androgens, dihydrotestosterone (DHT) or 11-ketotestosterone (11-KT) in order to identify genes that are differentially regulated by these steroid hormones. Using subtractive hybridization on livers of fish treated with DHT against vehicle control, many novel LMB genes were cloned. These genes were added to our gene library and arrayed. Six genes were up-regulated and five were down-regulated by both androgens. But, each androgen also regulated specific genes. One gene that was identified as a potential androgen marker was spermidine-spermine-N(1)-acetyltransferase that was up-regulated by both androgens. Determining which genes are responsive to natural androgens will help to identify biochemical pathways that are impacted.     

北海盆地第三纪深海扇的定量分析

北海盆地第三纪深海扇的定量分析Ｔ．Ｒｅｙｎｏｌｄｓ深海扇内富存有大量的碳氢化合物。因此，无论是从扇容积作为石油储层还是在生产期提供水的补给方面来说，它们的规模、几何形态、砂的体积、纯砂体积与总体积之比以及砂的连通性都是石油工业的关键问题。但是，至今深...     

The Japanese medaka (Oryzias latipes) model: applicability for investigating the immunosuppressive effects of the aquatic pollutant benzo[a]pyrene (BaP)

Despite the fact that BaP is a carcinogen, mammalian immunosuppressant, and ubiquitous aquatic pollutant, knowledge regarding the effects of BaP on the immune system of fish is still lacking. To begin to fill this gap, studies were conducted in medaka to examine the effects and mechanisms by which BaP exposure might alter host immunocompetence. Fish, exposed by IP injection of BaP (2-600 microg/g BW), were examined after 48 h for effects upon immune function and CYP1A expression/activity. Benzo[a]pyrene, at a concentration below that which increased levels of CYPIA expression/activity (2 microg BaP/g BW) suppressed lymphocyte proliferation. Concentrations of BaP at 20 and 200 microg/g BW. suppressed antibody-forming cell (AFC) numbers, superoxide production, and host resistance against bacteria. In contrast, exposure to the low affinity aryl hydrocarbon receptor (AhR) agonist, benzo[e]pyrene (BeP), neither induced CYP1A expression nor altered immune function. Given the lack of immunosuppressive effects produced by BeP, and the fact that exposure to the AhR antagonist (and CYP1A inhibitor) alpha-naphthoflavone (ANF) ameliorated the suppressive effects of BaP upon AFC numbers, the AhR pathway (including CYP1A-mediated production of reactive BaP metabolites) appears important in mediating BaP-induced immunotoxicity in fish, as in mammals. In the past, the medaka has proven a successful model for assessing carcinogenic agents. These studies have demonstrated its utility for also determining the immunosuppressive effects of an important aquatic contaminant.     

Susceptibility to genetic damage and cell types in Mediterranean mussels

Micronucleus (MN) frequency is generally accepted as a marker of chromosomal damage and has been studied in a variety of cells and species. In previous work, we detected significant dose-related MN increases in the epithelial-like gill cells and agranular haemocytes of Mytilus galloprovincialis treated with benzo[a]pyrene, a well-known mutagenic pollutant. In addition, we have studied micronuclei and other nuclear abnormalities in mussels collected from the Venice lagoon (Italy). Frequency changes, possibly related to genotoxic/toxic stress, in both granular and micronucleated cells from gills and haemolymph, were detected. Environmental data suggest the effect of genotoxic pollutants and the importance of cell replication in the interpretation of micronucleus frequencies.     

西北巴拉望GSEC66.区块深水区石油潜力

本文论述了Philodfill GSEC66区块，即人们称之为“西北Malampaya区块”的石油潜力评价结果。相信该区块存在主要烃类聚集所必需的要素。可能的烃源岩是深水尼多灰岩，早第三纪同裂谷期前尼多沉积和前第三纪沉积。潜在储集层包括尼多礁隆、尼多和前尼多碎屑岩和，或破碎碳酸盐岩，早中新世Galoc碎屑浊积岩和前尼多沉积。该地区识别出的主要油藏类型是晚渐新世一早中新世礁和侵蚀／喀斯特碳酸岩高地。在对已有的1982年以前地震资料评价后，又采集了1，625km的地震资料。新的地震资料解释后，在这一地区划出了11个预选区，其中2个勘探区（Santa Monica和Ipil勘探区）。Santa Monica是在尼多灰岩上圈出的一个十字倾角圈闭，垂直闭合高度超过457m，闭合面积超过83km^2。Ipil勘探区发育在尼多灰岩台地面上，具缓倾闭合特征，闭合面积超过47km^2，垂直闭合高度122m。这个大型勘探圈出的技术价值与菲律宾十分有利的深水勘探条件及其邻近的即将开发的Malapaya—Camago油气田结合，使这一区块成为今后勘探工作中一个极具前景的地区。     

高能波控陆架的风暴沉积和风暴层序——以澳大利亚东南部陆架为例

根据浅层地震和钻孔资科,将澳大利亚东南部研究地区高能波控陆架风暴沉积分为直立风暴层、叠瓦状风暴层、无序风暴层、水平风暴层和纹层状风暴层等五类.各类风暴层及风暴层与非风暴层组合而成悬移风暴层序、悬移-推移混合风暴层序和推移风暴层序三大类.中陆架风暴沉积与现今近岸和水下岸坡相似,岩性、岩相和古生物属海岸带,风暴沉积厚,底板埋深大,组成物质粗.推断中陆架风暴沉积当形成于冰后期海侵、该地带处在近岸带和水下岸坡环境时,此时海面上升速度可能减缓,或趋近于零,甚至可能有短时间的下降.     

印度洋马尔代夫礁岛演化的新模式

珊瑚礁岛是由钙质砂砾堆积形成的，这些砂砾构成了环礁和其他礁台的表层。礁岛的低海拔高度、小规模尺寸和对局部礁积物的依赖性使它们深受气候变化和海平面上升的影响，因此，礁岛的稳定性对于只能在这种岛上居住生存的珊瑚礁国家来说是需要考虑的头等大事。我们要增强对礁岛沉积历史的研究，得出将来解决礁岛稳定性的最佳方案。     

西南太平洋彭林海盆表层和埋藏锰结核成分和生长史

本文所研究的样品由《Hakurei—maru》号科学考察船进行的GH80—1和GH83—3两个航次所采集．这两次航行的目的是作为日本“中太平洋锰结核调查”五年计划(1979—1983)任务的一部分，在威克-塔希提横断层上进行地质调查(GSJ)。     

第23章 DNS模式:一种为大洋波浪预报而改进的谱模式

23.1 介绍 模拟与频率和方向相关的大洋波谱F（f,θ）的工作是极其困难的。唯一有点统一的理论（Hasselmann,1967）也还没有简化成一种实用形式。这篇研究中所探讨的是结合零散的,现有的理论,以达到这样一种目的:即发展一种与我们目前对风浪成长谱的了解相一致 ·95.的波谱模式。本章中我们将讨论一种离散的谱模式,这种模式采用了一种改进的能量传播的特征方法。数值方法最早是由Barnett等（1969）提出的。