Distribution and expression in vitro and in vivo of DNA vaccine against lymphocystis disease virus in Japanese flounder(Paralichthys olivaceus)

Lymphocystis disease,caused by the lymphocystis disease virus (LCDV),is a significant worldwide problem in fish industry causing substantial economic losses.In this study,we aimed to develop the DNA vaccine against LCDV,using DNA vaccination technology.We evaluated plasmid pEGFP-N2-LCDV1.3 kb as a DNA vaccine candidate.The plasmid DNA was transiently expressed after liposome transfection into the eukaryotic COS 7 cell line.The distribution and expression of the DNA vaccine (pEGFP-N2-LCDV1.3kb) were also ana...     

RESPONSE OF TRIASSIC SEQUENCE STRATIGRAPHY OF LOWER YANGTZE TO THE COLLISION BETWEEN THE YANGTZE PLATE AND THE NORTH CHINA PLATE

The characteristics of the Triassic sequences developed in the Lower Yangtze area display some great changes in both environment and climate. The change of environment was a transition from marine to continent via alternating environments. The change of climate was a transition from tropic （torrid） to warm and wet climate via subtropic dry climate. The type variations of the sequences were from the marine sequences to the continental sequences, corresponding to the changes of environments and climates. Sequence 1 is a type II of sequence of mixed elastic and carbonate sediments; sequence 2 is a type I of sequence of carbonate platform; sequence 3 is a type I of sequence of carbonate tidal flat-salt lagoon, sequence 4 is a type iI of sequence of lacustrine within marine layers, and sequence 5 is a sequence of lacustrine-swamp. The development, distribution and preservation of those sequences reveal the tectonic controls and their changes in the background. The collision between the Yangtze plate and the North China plate was a great geological event in the geological history, but the timing of the collision is still disputed. However, the characteristics of Triassic sequence stratigraphy and sea level changes in the Lower Yangtze area responded to this collision. The collision started at the beginning of middle Triassic and the great regression in the Lower Yangtze area started 22Ma earlier than those in the world. The tectonic conditions occurred before and during the collision controlled the development of sequences and type changes.     

贵州织金洞世界地质公园喀斯特成景机制及模式研究

本文将织金喀斯特分为8大类:洞穴、峡谷、天生桥、天坑、高峰丛、丘陵、单面山与象形山、水文遗迹等,它们以下三叠统海相碳酸盐岩为物质基础,有序、集中分布于织金洞、绮结河、东风湖三片相对独立,却又以绮结河为纽带紧密相连的喀斯特区域内,共同构成一个以洞穴、峡谷、天生桥、天坑为核心,形态雄伟、典型、优美、珍稀的高原喀斯特景观群。同时在对公园区域成景背景进行分析和研究的基础上,认为织金喀斯特的形成演化,始于古近纪,以三处喀斯特区域相互之间水力联系的演变为主轴,历经四个阶段:初始阶段(古近纪期间,喀斯特地层出露)→雏形阶段(分轴型离散岛屿式成景过程)→重要发育阶段(主轴型离散岛屿式与集合型岛屿式成景过程)→现代喀斯特阶段。期间,三个喀斯特区域产生不同的喀斯特响应过程:(1)织金洞区域,以织金洞地下河的演变为主轴,历经横向裂隙式岩溶水→地下河道→抬升形成化石洞穴/洞穴景观发育阶段/袭夺地表河(古新寨河)→地下河大规模发育→抬升形成化石洞穴/被袭夺阶段/→地下河小规模发育/洞穴景观发育等不同的地下岩溶循环发育阶段,形成了规模宏大的4层迷宫式化石洞穴系统—织金洞洞穴系统,洞内次生化学沉积物类型齐全,形态优美、独特,体量巨大,现代总体属于已停止发育的化石型洞穴喀斯特区域。(2)绮结河区域,以绮结河的演变为主轴,历经地表流水各向分散流动阶段→地表河阶段→地下河阶段→地下河改道/袭夺阶段→地下河大规模发育阶段→改道/明流与伏流交替阶段等不同的地表与地下岩溶交替循环发育阶段,峰丛、洞穴、峡谷、天坑、天生桥等地貌有序分布于河谷上或其两侧,协同共生,现代总体属于地表与地下喀斯特正在相互交替发育的区域。(3)东风湖区域,以六冲河的演变为主轴,历经持续、漫长、大规模、高强度的横向—纵向侵蚀、切割,峰丛、丘陵、象形山、单面山、峡谷有序分布于其上宽下窄的河谷上或其两侧,共生发育,现代总体属于正在发育的地表喀斯特区域。(4)整个公园,以三处喀斯特区域相互之间水力联系的演变为主轴,历经四个不同的喀斯特阶段,及各区域不同的喀斯特响应过程,形成独特的织金高原喀斯特景观群,现代总体上正在分区进行着三种不同的喀斯特过程。在上述分析的基础上,提出分轴型、主轴型、离散型和集合型等四种岛屿式成景机制。同时,通过与相似、典型岩溶区域(如广西乐业、重庆武隆等)的成景模式进行对比,将织金喀斯特成景模式归纳为"相邻岛屿式成景模式"。     

南科1井第四系暴露面特征及其与海平面变化的关系*

珊瑚礁地层中的暴露面是海平面变化的忠实记录, 对地层层序的划分和研究珊瑚礁体的生长发育过程具有重要意义。本文基于手标本观察与薄片鉴定、碳氧同位素和矿物组成分析, 识别了南沙美济岛南科1井第四纪生物礁碳酸盐岩地层中的典型暴露面, 剖析了主要暴露面与海平面变化及珊瑚礁发育演化的关系。在南科1井(NK-1)第四纪地层中典型暴露面附近以出现大量溶蚀孔穴和红褐色或锈黄色钙质结核为特征, 其碳氧同位素偏负, 并富集Al、Th、Fe和稀土元素, 具有典型碳酸盐岩风化壳的特征。南科1井全新世礁体发育在晚更新世暴露面之上, AMS¹⁴C和U-Th年龄数据证实美济岛全新世珊瑚礁生长时段与南海其他珊瑚岛礁一致, 主要发育在8.2~4.7ka时期, 该时段海平面的缓慢上升为珊瑚礁连续垂向生长提供了有利环境。Sr同位素和古地磁年龄标定的南科1井更新世地层中的主要暴露面时代与南沙永暑礁和西沙群岛珊瑚礁地层中的暴露面时代基本一致, 主要暴露面对应于全球第四纪低海平面时期。     

AIR-SEA COUPLING FEATURES FROM THE WARM PHASE TO THE COLD PHASE OF ENSO CYCLES IN THE SEVENTIES TO THE EIGHTIES

Using the sea surface temperature and wind anomalies(SSTA and SSWA for short)of thetropical Pacific from January 1970 to December 1989,main spatial patterns of tropical PacificSSTA and SSWA coupling features in the transform course from the warm phase to the cold phaseof El Nino-southern Oscillation(ENSO)cycles are discussed.The main conclusions are as follows:(1)air-sea coupling patterns at the mature stage of ElNino(La Nina)are main spatial ones of tropical Pacific SSWA and SSTA coupling:(2)at themature stage of El Nino,the interaction of the anticyclonic anomaly wind,generated by the forcingof distinct meridional SSTA gradient in the Northern Hemisphere tropical central Pacific.with theCalifornia cold current and SSTA is mainly responsible for weakening of El Nino;(3)the secondsea temperature increase along the South American coast in the decaying course of El Nino resultsfrom the eastward movement of the weakened positive SSTA in the tropical central-eastern Pacificforced by anomalous west wind stress:(4)La Nina results from the joint effect of Walkercirculation,Ekman drift and negative SSTA in the tropical central-eastern Pacific.     

来宾岩溶地下水分布特征及开发利用

来宾县多年来主要依靠地表水治理干旱,但地表水灌溉不到的地区,其干旱问题一直得不到解决。本文详细分析了来宾岩溶地下水富集的控制因素和分布特征,认为来宾蕴藏着丰富的岩溶地下水资源,且在地域上恰好弥补了无地表水资源的空缺。并根据岩溶地下水的赋有特征提出了开发岩溶地下水的几种方式。