Evidence from heterologous expression of glutathione S-transferases A and A1 of the plaice (Pleuronectes platessa) that their endogenous role is in detoxification of lipid peroxidation products

cDNA clones for glutathione S-transferases A (GST-A) and A1 (GST-A1) from plaice (Pleuronectes platessa) were expressed as N-terminally 6XHis tagged proteins in Escherichia coli and purified to homogeneity from Ni-NTA silica. GST-A was an efficient catalyst for conjugation of unsaturated alkenals derived from peroxidation of polyunsaturated fatty acids with the highest activity observed with trans-non-2-enal (8 micromol min(-1) mg(-1)). GST-A1 was a very efficient Se-independent glutathione peroxidase with an activity towards cumene hydroperoxide of 25 micromol min(-1) mg(-1). Although the enzymes exhibited moderately high activities towards the model substrate 1-chloro-2,4-dinitrobenzene (CDNB) they exhibited little or no activity towards other common prototypical xenobiotic substrates. Together with data for ontogeny, tissue distribution and inducibility of these enzymes, we contend that a primary function of these enzymes is protection from the harmful effects of lipid peroxidation products generated naturally or exacerbated by xenobiotic exposure.     

两株琼胶酶高产细菌的筛选和鉴定

从海洋环境中筛选得到两株琼胶酶的高产菌株，通过形态观察和生理生化反应，确定它们属于弧菌属，通过BIOLOG细菌鉴定系统鉴定，并同弧菌属标准菌株分析比较，确定上方宝剑两株菌都是塔式弧菌（Vibrio tubiashii)，这两株菌在碳源利用方面存在差别。     

布莱克海岭区天然气水合物的评价

布莱克海岭位于美国东南加利福尼亚近海区,该区天然气水合物矿藏可用的开采方案有3种。这些方案为深水环境中天然气水合物矿藏的开采提供了可能的途径。1布莱克海岭区的天然气水合物过去20年中,在布莱克海岭区收集了大量的天然气水合物资料,包括地震勘探资料和11口井的钻井资料     

Effects of prolonged exposure to 4-tert-octylphenol on toxicity and indices of oestrogenic exposure in the sand goby (Pomatoschistus minutus, Pallas)

Alkylphenolic compounds are present in estuarine and marine environments. They are moderately bioaccumulative, and oestrogenic to fish following three week, in vivo, exposures. The effects of prolonged exposure are, as yet, unclear. Sand goby (Pomatoschistus minutus) were exposed to measured concentrations of 4-tert-octylphenol (OP) in a 28-day, dose-ranging study, and a six month temporal response study. Following 28-days exposure to measured 31+/-6 or 101+/-47 microg l(-1) OP (mean+/-SD), immature male sand goby showed elevated vitellogenin (VTG) mRNA expression. Plasma alkali-labile phosphate concentrations were elevated in both sexes and were higher in females than males. Although measured OP concentrations were variable, they were significantly different for each treatment and a range of concentration-dependent effects were observed. The LC(50) for chronic OP exposure (8 weeks) was 29+/-6 microg l(-1), and both sexes demonstrated concentration and duration dependent increases in VTG mRNA expression. Exposure to 28+/-20 microg l(-1) OP (median+/-95% CI) for 6 months inhibited development of male nuptial colouration and sperm duct glands. These findings are discussed in relation to environmental concentrations of alkylphenols and ecological concerns.     

烟台海胆体内的纤毛虫和太平洋海胆体内纤毛虫的地理分布

1957年夏,中苏海洋动物综合考察队在黄海沿岸烟台地区工作时,参加考察队的阿·阿·斯特列科夫教授采集了海胆肠内的纤毛虫,并以此作为这篇文章的材料。在本文内将提出所见到的纤毛虫名称,并分析它们在太平洋地理分布的情况。     

Variations of antioxidant efficiency and presence of endosymbiotic diatoms in the Antarctic porifera Haliclona dancoi

The role of endosymbiotic diatoms as pro-oxidant stressors in porifera has been investigated in the Antarctic sponge Haliclona dancoi in which the presence of diatoms is influenced by marked seasonal variations during the austral summer. Both chlorophaeopigments and frustules were absent in sponge tissues sampled in early November at the beginning of the summer and increased from the mid of December with slightly shifted temporal trends. The efficiency of antioxidant defenses in the sponge showed a marked response to symbionts with clearly enhanced values corresponding to the peak of diatoms.     

通过对重力波曳力的参数化来减小数值天气预报和大气环流模式中的系统性误差

一、引 言 由于地形性激发的中尺度重力波的中断,在加拿大气候中心大气环流谱模式中有时候采用了波的曳力效应的参数化。这一参数化的细节以及它在大气环流模式气候模拟中影响的讨论,可见Boer等人(1985)的工作。 最近,人们利用激发重力波次尺度地形的更好的描绘,来修改这一参数化方案。还有,使用Lindzen(1981)的波饱和概念来描述曳力效应。这一关于地形重力波曳力参数化的作法已在加拿大气候中心的大气环流谱模式中广泛试验,在加拿大气象中心的业务的谱数值预报模式中也正在普遍进行试验。本文的目的所在于概述在这些模式中正在普遍使用的波的曳力方案,并且证明这一方案在消除中期预报和大气环流模拟的系统性误差方面的用处。     

海南岛珊瑚礁的主要类型

一、前言 中苏海洋生物考察团,于1958年3—6月在中国南海的海南岛进行了考察工作,对该地区的海洋潮间带动物群的栖息条件和沿岸的动物生态学进行了调查研究。工作中除考察了岩石、红树丛、沙滩及泥滩的生物群落外,同时考察了热带海洋中典型的生物群落——珊瑚礁。     

黄海多毛类动物地理学的初步研究

1.黄海多毛类区系包含有中国沿岸特有地方性种,黄海和日本沿岸的特有地方性种,广温性热带暖水种,寒温带冷水种和广布种等不同性质成分。 2.值得提出的是太平洋东西两岸种在黄海的发现,计有19种。这些种的分布应该考虑不仅是由于历史的成因,在某种程度上幼体也许有可能随当前自日本沿岸流向美洲沿 岸的黑潮传布过去。多毛类有些种幼虫期很长,并且能随海流分布至深海(Thorson,1950)。 3.根据与邻近海区区系的比较,黄海多毛类与日本沿岸,特别是日本本州北部金华山附近的女川湾和松岛湾一带的区系最相近似,女川湾和松岛湾一带属亲潮寒流和黑潮暖流交汇区,因之在种类组成上不仅有来自南方的暖水种,同时,还有北方的冷水种,具有混合区系特征。黄海——本州北部应属同一地理区划。 4.黄海多毛类具有十分显著的混合区系特征,与日本的情况不同,黄海的暖水种多分布在潮间带,冷水种大部分在较深水域,井与深水区终年存在的冷水团有关。 5.黄海北部和中部应属北太平洋北温带和印度西太平洋热带区之间的过渡区。     

Variational iteration solving method for El Nio phenomenon atmospheric physics of nonlinear model

1 IntroductionThe El Ni’o atmospheric physics oscillation is anabnormal phenomenon involved in the tropical Pacificocean- atmosphere interactions. Studies on the El Ni(ophenomenon are very attractive (Lin et al., 2000; Linet al., 2001, 2002; Wang, 2001; Feng et al., 2001;Feng et al., 2002; Liu et al., 2002; Wei and Chen,2003; Xie et al., 2002; Zhu et al., 2002; Pu et al.,2003; Gu et al., 2004; Yu and Liu, 2004). Lin and Mo(2004), Mo and Lin (2004) and Mo et al. (2004) alsostudied a s…