栉孔扇贝精巢组织细胞的体外培养及特征分析

本研究建立了一个栉孔扇贝精巢组织细胞的原代培养体系,鉴定了原代细胞的功能基因表达特征。结果显示,原代培养体系中的精巢细胞以圆形细胞为主,直径为4~11μm,其在体外已存活9个月以上。使用栉孔扇贝vasa基因(生殖细胞标记基因)的地高辛RNA探针和原位杂交技术检测,约98%的原代细胞呈阳性。利用免疫组化技术鉴定了栉孔扇贝SCP3、KLF4、PIWI、C-MYC、SOX9和SOX17在这些细胞中的表达特征,发现这些靶蛋白在精巢原代细胞中的表达特征与栉孔扇贝精巢中的细胞定位基本一致。上述结果表明,本实验所获得的原代细胞以生殖细胞为主,它们保留了栉孔扇贝雄性生殖细胞原有的基因表达特征,可以用于贝类精子发生和性别相关的功能基因研究。     

Local Oceanic Vertical Deflection Determination with Gravity Data Along a Profile

Deflections of the vertical (DOVs) over oceans cannot be directly measured, which restricts their applications. A local covariance function of anomalous potential is put forward in this paper in conjunction with the least-squares collocation (LSC) method to compute the oceanic DOVs utilization of oceanic gravity data along a profile. The covariance functions of gravity field quantities have been derived directly as functions of x, y and z without the need to introduce coordinate transformations corresponding to along- or cross-profile components. In the proposed methodology, gravity data along a profile were used to calculate the residual gravity anomaly using the remove-compute-restore technique. The residual gravity anomaly was used to calculate the parameters of the proposed covariance function of the local anomalous gravity field, which was used in the LSC to compute the residual DOVs along the profile. The residual DOVs added model DOVs to recover the DOVs along the profile. The results of a simulation experiment prove that the proposed methodology is feasible and effective.     

我国大黄鱼养殖产业发展研究

文章对我国大黄鱼养殖产量及分布、养殖模式、投入产出情况、收购价格变动和进出口情况进行分析，总结了几项产业中存在的问题，包括：养殖结构亟待调整、关键养殖技术欠缺、鱼苗成活率偏低、市场中存在逆向选择。在此基础上，提出积极调整养殖结构与养殖密度、提升合作社与试验站职能、加强养殖技术与鱼药研发力度、培育推广优质大黄鱼苗种、促进可追溯体系建设的产业发展建议。     

连云港近海紫菜养殖区遥感识别、空间变异和驱动分析

为了科学有效地掌握海域使用信息、管理海域权属,为海域资源调查、管理、规划、保护、合理利用提供服务,为违法、违规、违章用海项目得到有效遏制,减少用海矛盾,有必要开展海域使用动态监测,作者以连云港近海为研究区,利用4个时相Landsat遥感影像(2002、2008、2013、2016年)通过面向对象算法提取弱水背景下的紫菜养殖区信息,揭示空间分异规律,并分析其驱动原因。研究结果表明:4期影像提取连云港近海紫菜养殖区信息的精度分别是87.5%、92%、91.7%和94.6%。紫菜养殖区空间分布的变化规律为紫菜养殖面积不断增加且整体向深海方向推进。赣榆港口航运区和田湾核电站特殊利用区存在少量紫菜养殖区,项目用海不符合连云港市海洋功能区划(2016～2020年)。连云港近海紫菜养殖区空间分异的驱动因子主要是自然条件、养殖技术、海洋经济和社会发展四大因素。     

Landsat长时间序列的阳澄湖湖面围网时空变化

准确提取湖泊围网区域的时空分布信息对湖泊的保护和可持续发展具有重要意义。本文以阳澄湖为研究区域,收集该地区1984年—2017年所有的Landsat 5和Landsat 8影像(共计396景),提出了结合光谱和纹理特征的围网提取新算法,同时利用时间序列滤波消除年际间因数据不一致造成的偏差。以高清影像人工解译作为参考,阳澄湖围网提取结果的生产者精度在72.57%—88.53%,用户者精度在79.79%—98.10%,围网面积变化与文献记录吻合。结果表明,阳澄湖围网经历了"无围网期"(1984年—1994年)、"快速增长期"(1994年—1998年)、"巅峰期"(1999年—2002年)、"快速下降期"(2003年—2006年)和"稳定期"(2007年—2017年)5个阶段,最高达到100 km2,目前稳定在30 km2;通过研究围网区植被指数发现,2002年之后围网区浮水植物的种植面积增大;通过对比水质数据发现,2002年至今持续15年的围网拆除并未使阳澄湖恢复到80年代无围网养殖时期的II类水,其水质依然处于Ⅲ—Ⅳ类。因此在湖泊养殖开发过程中,政府应该坚持可持续发展道路,在不破坏湖泊水质的基础上发展湖泊经济。     

不同流速和苗种规格对缢蛏平面流中间培育效果的影响

为了优化蛏苗集约化平面流中间培育技术,研究了不同进水流速和苗种规格对缢蛏中间培育效果的影响,并分析了集约化平面流中间培育系统的水质状况。结果显示,不同进水流速对缢蛏稚贝生长影响显著,稚贝生长速率随进水流速增加而增加,但成活率下降。通过流速与成活率和体质量日增生长量的线性回归分析,估算0.163L/s为适宜的进水流速。在适宜流速和相同放苗重量下,大规格苗种(8万粒/kg)生长速度显著高于小规格苗种(18万粒/kg),但因为小规格组放苗数量多,小规格组单位面积质量较其高出23.72%。除低流速组以外,平面流中间培育过程对叶绿素a和铵态氮有良好的去除效果,去除率分别达到36.99%和3.88%以上,这表明平面流集约化中间培育在利用海水池塘水体进行苗种中间培育的同时,也起到了池塘养殖水体的净化作用。综合认为,在养殖密度0.5kg/m^2、流速0.163 L/s的培育条件下,可以保证水体自污染程度较低,缢蛏苗种生长较快,成活率在73.12%以上。     

Enhancement of backtracking search algorithm for identifying soil parameters

In this paper, an enhanced backtracking search algorithm (so-called MBSA-LS) for parameter identification is proposed with two modifications: (a) modifying the mutation of original backtracking search algorithm (BSA) considering the contribution of current best individual for accelerating convergence speed and (b) novelly incorporating an efficient differential evolution (DE) as local search for improving the quality of population. The proposed MBSA-LS is first validated with better performance than the original BSA and some other typical state-of-the-art optimization algorithms on a benchmark of soil parameter identification in terms of effectiveness, efficiency, and robustness. Then, the efficiency of the MBSA-LS is further illustrated by two representative cases: identifying soil parameters from both laboratory tests and field measurements. All comparisons demonstrate that the proposed MBSA-LS algorithm can give accurate results in a short time. Finally, to conveniently solve the problems of parameter identification, a practical tool ErosOpt for parameter identification is developed by integrating the proposed MBSA-LS and some other efficient algorithms for readers to conduct the parameter identification using optimisation algorithms.     

Influence of heterogeneity on unsaturated hydraulic properties: (1) local heterogeneity and scale effect

Spatial heterogeneity is ubiquitous in nature, which may significantly affect the soil hydraulic property curves. The models of a closed‐form functional relationship of soil hydraulic property curves (e.g. VG model or exponential model) are valid at point or local scale based on a point‐scale hydrological process, but how do scale effects of heterogeneity have an influence on the parameters of these models when the models are used in a larger scale process? This paper uses a two‐dimensional variably saturated flow and solute transport finite element model (VSAFT2) to simulate variations of pressure and moisture content in the soil flume under a constant head boundary condition. By changing different numerical simulation block sizes, a quantitative evaluation of parameter variations in the VG model, resulting from the scale effects, is presented. Results show that the parameters of soil hydraulic properties are independent of scale in homogeneous media. Parameters of α and n in homogeneous media, which are estimated by using the unsaturated hydraulic conductivity curve (UHC) or the soil water retention curve (WRC), are identical. Variations of local heterogeneities strongly affect the soil hydraulic properties, and the scale affects the results of the parameter estimations when numerical experiments are conducted. Furthermore, the discrepancy of each curve becomes considerable when moisture content becomes closer to a dry situation. Parameters estimated by UHC are totally different from the ones estimated by WRC. Copyright © 2012 John Wiley & Sons, Ltd.     

基于线要素动态化简的匹配算法比较与评价

矢量线要素数据来源多样,细节层次不一,限制了已有匹配算法正确率的提高,同时也给算法评价带来困难。化简可以减少线要素细节层次,提取其主要形态,据此提出一种基于线要素动态化简的匹配算法评价新方法。对不同匹配算法采用相同数据,在相同化简算法支撑下进行匹配,从而实现对不同匹配算法的评价。首先,阐述动态化简方法提取线要素主要形态的过程;其次,利用动态化简分别辅助4种已有匹配算法,获取每个匹配算法的最优匹配正确率;最后,将4种匹配算法的原始匹配结果与加入动态化简后的匹配结果进行对比,分析化简对匹配结果的影响,并把该影响运用到匹配算法的比较和评价中来。其中,1通过匹配正确率变化、误匹配等分析了匹配算法的数据适用性;2通过化简比例系数K变化时新增匹配数量的统计,评价了匹配算法对线要素局部细节的敏感程度并提出该指标的量化方法;3结合匹配算法采用的匹配相似度指标对其作出评价。     

局部区域模型重力异常快速算法

地球重力场位系数模型可以用于计算局部重力扰动场元。然而随着地球重力场模型阶次的提高、局域重力场计算范围的增大,其计算速度往往不能满足工程需求。针对这一问题,在对位系数模型泰勒级数展开的基础上提出了采用向量运算、混合编程的方法,同时对连带勒让德函数Belikov递推方法中与经纬度无关的量进行了预先计算,有效提高了计算速度。提出的方法对于利用超高阶次重力场模型快速解算大范围、高分辨率重力场元数据以及累加求和计算具有一定的参考与借鉴意义。