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21.
Fucoidan is such a polysaccharide that its hydroxies are easy to combine with lanthanons ion (CeⅣ) to form complex. This work obtained the complexes of three fucoidan oligosaccharides with different molecular weights F1( >5 000), F2(1 000-5 000) and F3( < 1 000) by hydrolyzing Oligosaccharide collagen with sulfuric acid. It is found that the fucoidan oligosaccharide F3 can form complex with more CeⅣ than F1 and F2. Hydrolyzing collagen with the complex was carried out to produce amino acid and peptides. All the three fucoidan oligosaccharide complexes with CeⅣ( F1, F2, F3) can catalyze by the artificial hydrolytic enzyme, and the activity of the complex of F3 is the highest. 相似文献
22.
Collagen of squid (Ommastrephes bartrami) skin was examined in the present study. Histology showed that collagen fiber in the skin was partially cross-linked with
muscle fiber. Acid-solubilized collagen (ASC) and pepsin-solubilized collagen (PSC) were extracted from the skin and characterized.
The results of amino acid composition and electrophoretic patterns revealed that ASC and PSC were both type I collagen, containing
α1 and α2 chains. FTIR (fourier transform infrared spectroscopy) investigations confirmed the existence of helical arrangements
in PSC of squid skin. The denaturation temperature (Td) and shrinkage temperature (Ts) of PSC were 29.4°C and 52.8°C, respectively. 相似文献
23.
We present here a pilot study to examine trophic level effects and migration patterns at the middle Shang Dynasty site of Xiaoshuangqiao in Henan Province using δD results combined with δ13C and δ15N values. A total of 33 specimens (17 humans, 7 cattle, 5 pigs, 3 sheep, 1 dog) of bone collagen were isotopically analyzed for δ13C, δ15N, and dD values. A strong positive correlation (R2 = 0.94) between mean δ15N and δD values of herbivores (cattle and sheep), omnivores (pig), carnivores (dog) and humans was observed. The δD results were found to increase by ~10‰ to 20‰ from herbivores to omnivores to carnivorous, evidence that collagen δD results are a useful indicator for the study of trophic levels and dietary patterns at archaeological sites. The δD results were also used to examine the origins of two different groups of individuals buried at Xiaoshuangqiao. Individuals buried in sacrificial pits of district V had mean δD values (?47.0 ± 2.9‰, n = 11) that were significantly (p = 0.049) elevated compared to the people buried in the stratigraphy of district IX (?51.3 ± 3.3‰, n = 3), indicating that they were ingesting water from different locations. In addition, the D values of the people buried in the stratigraphy were similar to the pigs (?54.5 ± 4.2‰, n = 5) at Xiaoshuangqiao, suggesting that they were most probably of the local population, and that the individuals buried in the sacrificial pits were most possibly from the coast and prisoners of the Dongyi (“东夷”) people. Thus, δD results have the potential to examine human origins and migration patterns and should be increasingly used in conjunction with δ13C and δ15N values at archaeological sites. 相似文献
24.
Rheological and Structural Properties of Sea Cucumber Stichopus japonicus During Heat Treatment 总被引:6,自引:0,他引:6
GAO Xin XUE Dongmei ZHANG Zhaohui XU Jiachao XUE Changhu 《中国海洋大学学报(英文版)》2005,4(3):244-247
Changes in tissue structure, rheological properties and water content of raw and heated sea cucumber meat were studied. Sea cucumber Stichopus japonicus was heated at 25℃, 70℃ and 100℃ water for 5 min. The structural changes were observed using a light microscope and the rheological parameters (rupture strength, adhesive strength and deformation) determined using a texture meter. Microscopic photograph revealed that the structural change of heated meat was greater than that of raw meat. The rupture strength, adhesive strength and deformation of raw meat were smaller than those of the heated meat. Meanwhile, rheological parameters showed positive correlation with heating temperature. These changes are mainly caused by thermal denaturation and gelatinization of collagen during heating. These changes were also evidenced in observations using a light microscope and differential scanning calorimetry. 相似文献
25.
Fucoidan is such a polysaccharide that its hydroxies are easy to combine with lanthanons ion (Ce^IV) to form complex. This work obtained the complexes of three fucoidan oligosaccharides with different molecular weights F1 (〉5 000), F2 (1 000-5 000) and F3 (〈 1 000) by hydrolyzing Oligosaccharide collagen with sulfuric acid. It is found that the fucoidan oligosaccharide F3 can form complex with more Ce^IV than F1 and F2. Hydrolyzing collagen with the complex was carried out to produce amino acid and peptides. All the three fucoidan oligosaccharide complexes with CeIV( F1, F2, F3) can catalyze by the artificial hydrolytic enzyme, and the activity of the complex of F3 is the highest. 相似文献
26.
XU Jiachao GAO Xin ZHANG Zhaohui LI Zhaojie HUO Lihua and XUE Changhu Department of Food Science Engineering Ocean University of China Qingdao P. R. China 《中国海洋大学学报(英文版)》2006,(2)
Fucoidan is such a polysaccharide that its hydroxies are easy to combine with lanthanons ion (CeIV) to form complex. This work obtained the complexes of three fucoidan oligosaccharides with different molecular weights F1(>5000), F2 (1000-5000) and F3(<1000) by hydrolyzing Oligosaccharide collagen with sulfuric acid. It is found that the fucoidan oligosaccharide F3 can form complex with more CeIV than F1 and F2. Hydrolyzing collagen with the complex was carried out to produce amino acid and peptides. All the three fucoidan oligosaccharide complexes with CeIV( F1, F2, F3) can catalyze by the artificial hydrolytic enzyme, and the activity of the complex of F3 is the highest. 相似文献
27.
ZHUANG Yongliang LI Bafang ZHAO Xue College of Food Science Engineering Ocean University of China Qingdao P. R. China 《中国海洋大学学报(英文版)》2009,8(2)
Fish skin collagen hydrolysates (FSCH) were prepared from walleye pollock (Theragra chalcogramma) using a mixture of enzymes, namely trypsin and flavourzyme. The degree of hydrolysis of the skin collagen was 27.3%. FSCH was mainly composed of low-molecular-weight peptides and the relative proportion of <1000Da fraction was 70.6%. Free radical and oxygen species scavenging activities of FSCH were investigated in four model systems, including diphenylpicrylhy-drazyl radical (DPPH), superox-ide anion radical, hydroxyl radical and hydrogen peroxide model, and compared with that of a native antioxidant, reduced glutathione (GSH). FSCH was also evaluated by water-absorbing and water-holding capacity. The results showed that FSCH was able to scav-enge free radical and oxygen species significantly and to enhance water-absorbing and water-holding capacity remarkably. Therefore, FSCH may have potential applications in the medicine and food industries. 相似文献
28.
WANG Ning HU YaoWu HOU LiangLiang YANG RuiPing SONG GuoDing WANG ChangSui 《中国科学:地球科学(英文版)》2014,57(5):1039-1047
In current palaeodietary research, gelatinization is the main method to extract insoluble collagen(ISC) from ancient bones. However, the degradation products of ISC, i.e., soluble collagen(SC), is often neglected and abandoned. In this work, we try to separate the extracts of ancient bones using gel chromatography and compare the contents of carbon and nitrogen, atomic C/N ratio, and stable carbon and nitrogen isotopic values of the extracts from three peaks to determine which peak can be attributed to SC. At last, the potential application of SC in palaeodietary research is discussed based on the comparison of stable isotopic values between ISC and SC. Among the three peaks, the second with the retention time between 17.5 min and 27.5 min had the most broad peak shape, indicating that the molecular weights of proteins collected were most variable. Besides, the contents of carbon and nitrogen and atomic C/N ratio of extracts in this peak were closest to the corresponding ISC. Based on the above, we conclude that the extract in second peak is SC. More important, the δ 13C and δ 15N values of ISC and SC are very similar. For ISC and SC with atomic C/N ratios within the normal range(2.9–3.6), the mean difference of δ 13C value was only(0.3±0.2)‰(n=2) while δ 15N value was(0.6±0.1)‰(n=2). Although the atomic C/N ratios of some SC are slightly beyond the normal range, the mean differences of δ 13C and δ 15N values were still only(0.4±0.1)‰ and(0.3±0)‰(n=2) respectively. These isotopic differences are quite below the isotope fractionation in one trophic level(δ 13C values of 1‰–1.5‰ and δ 15N values of 3‰–5‰), suggesting that SC had great application potentials in palaeodietary research. 相似文献
29.
3种鱼皮的基本成分及氨基酸组成分析 总被引:1,自引:0,他引:1
对军曹鱼(Rachycentron canadum Linnaeus)皮、罗非鱼(Oreochromis niloticus)皮、黄鳍金枪鱼(Thunnus albacares)皮的基本成分、氨基酸组成及胶原蛋白含量进行了分析。结果表明:三种鱼皮的粗蛋白质量分数分别为28.53%、33.14%、32.66%;氨基酸组成相似,其中甘氨酸含量(以残基计)最高,分别为32.9%、33.2%、34.6%,其次是丙氨酸,约占总氨基酸的14.1%、12.2%、12.7%;胶原蛋白的特征氨基酸——脯氨酸含量分别为10.8%、11.2%、11.2%;军曹鱼、罗非鱼及金枪鱼鱼皮胶原蛋白质量分数分别为20.09%、20.65%、22.64%(以湿基计),约占其粗蛋白的70.42%,62.30%和69.33%。 相似文献
30.
《第四纪科学杂志》2017,32(5):653-660