Using a historical aerial photograph analysis to inform trout habitat restoration efforts

Restoration of the upper Strawberry River included bank stabilization techniques because it was assumed that excessive bank erosion was degrading spawning habitat for Bonneville cutthroat trout (BCT). Using a long‐term aerial photograph record, the historical range of variability in bank erosion rates and channel geometry was determined, and this information was used to assess present‐day conditions and the rationale for restoration. Relative to historical variability, the channel planform was relatively stable and bank erosion rates were the lowest recorded in the post‐disturbance era. Although a historical loss of riparian vegetation coincided with a shift to a wider and more sinuous channel, lateral migration rates declined and the channel narrowed as riparian cover increased in the decades before restoration, indicating a process of natural recovery. Furthermore, it was found that the percentage of fine sediment in the streambed before restoration was insufficient to affect BCT spawning success. Together these results suggest that bank erosion and fine sediment did not affect the quality of spawning habitat or the abundance of BCT on the upper Strawberry River. The results highlight how a historical analysis can be used to identify the sources of habitat degradation and inform the selection of restoration goals and strategies. Copyright © 2011 John Wiley & Sons, Ltd.     

Produced water extracts from North Sea oil production platforms result in cellular oxidative stress in a rainbow trout in vitro bioassay

Produced water (PW) discharged from offshore oil industry contains chemicals known to contribute to different mechanisms of toxicity. The present study aimed to investigate oxidative stress and cytotoxicity in rainbow trout primary hepatocytes exposed to the water soluble and particulate organic fraction of PW from 10 different North Sea oil production platforms. The PW fractions caused a concentration-dependent increase in reactive oxygen species (ROS) after 1 h exposure, as well as changes in levels of total glutathione (tGSH) and cytotoxicity after 96 h. Interestingly, the water soluble organic compounds of PW were major contributors to oxidative stress and cytotoxicity, and effects was not correlated to the content of total oil in PW. Bioassay effects were only observed at high PW concentrations (3-fold concentrated), indicating that bioaccumulation needs to occur to cause similar short term toxic effects in wild fish.     

三倍体虹鳟(Oncorhynchus mykiss)卵巢分化及滞育的调控机制研究

尽管与性别决定和分化的相关基因在二倍体虹鳟中已被鉴定出来,如Cyp19a1a、Foxl2、Dmrt1、Amh和Sox9,但关于三倍体雌性虹鳟独特性腺表型及其相关性别控制基因的表达规律仍未得到深入研究,尤其是早期卵巢发育阻滞导致的生殖细胞去分化特征。因此,本研究以三倍体雌性虹鳟性腺发育中起重要调控作用的特异候选基因作为重点研究对象,辅助性腺体细胞与生殖细胞分化方向的组织学证据和类固醇激素表达规律,验证了各基因间的级联调控关系。结果表明,虹鳟三倍体与二倍体卵巢的分化特征基本一致。虹鳟二倍体与三倍体性腺分别在84dpf和98dpf就已分化为卵巢。但三倍体虹鳟卵巢分化存在障碍,早期发育停滞,卵原细胞及其滤泡细胞数量有限。Foxl2和Cyp19a1a在二倍体虹鳟卵巢和精巢中均有表达,它们在卵巢中表达量均呈逐渐升高趋势,但在精巢中的表达量非常低;这两个基因在雌性三倍体虹鳟性腺中的表达呈先升高后降低的趋势,其表达量均在8月龄时期达到最大。Dmrt1、Amh和Sox9在二倍体精巢和雌性三倍体卵巢中的表达均呈不断上调趋势,这些基因上调可能与血清睾酮含量的增加相关,因为在10月龄时期这两种鱼的血清含量相对较高,而这三个基因在二倍体虹鳟卵巢中的表达却呈下调趋势。因此本研究认为,维持鱼类卵巢分化需要持续高水平的雌激素,这可能是脊椎动物进化过程中的一个保守特征。可以推断雌性三倍体虹鳟卵巢滞育将导致其体细胞去分化,这对于Cyp19a1a表达及雌二醇合成存在抑制作用。因为这些雌激素是用来维持它们分化和继续发育的,可能产生一个负反馈圈,之后去分化会增强。     

ANNUAL CHANGES IN PLASMA LEVELS OF CORTISOL AND SEX STEROID HORMONES IN MALE RAINBOW TROUT, ONCORHYNCHUS MYKISS

ＩＮＴＲＯＤＵＣＴＩＯＮＴｈｅｒｅａｒｅａｌｏｔｏｆｐｕｂｌｉｓｈｅｄｐａｐｅｒｓｄｅｓｃｒｉｂｉｎｇｔｈｅａｎｎｕａｌｏｒｓｅａｓｏｎａｌｅｎｄｏｃｒｉｎｅｃｈａｎｇｅｓａｓｓｏｃｉ ａｔｅｄｗｉｔｈｒｅｐｒｏｄｕｃｔｉｖｅａｃｔｉｖｉｔｙｉｎｔｅｌｅｏｓｔｆｉｓｈｅｓ (Ｓｃｏｔｔｅｔａｌ.,1 980 ;Ｌｏｕｅｔａｌ.,1 984 ,1 986;Ｓｕｚｕｋｉｅｔａｌ.,1 997;Ｈｏｕ 1 998;Ｙａｒｏｎｅｔａｌ.,1 986) ,ａｎｄｅｖｉｄｅｎｃｅｔｈａｔｔｈｅｇｏｎａｄａｌｍａｔｕｒａｔｉｏｎｏｆｓａｌｍｏ ｎｉｄｆｉｓｈｗ…     

盐度变化对虹鳟和硬头鳟抗氧化酶活性的影响

本文研究了不同盐度驯化方式下虹鳟(Oncorhynchus mykiss,(99.44±0.26)g(简写为99 g))和两种规格硬头鳟(Oncorhynchus mykiss,(99.01±0.61)g(简写为99 g)和(394.50±1.16)g(简写为395 g))的血清抗氧化酶活性变化。实验设置4种盐度驯化方式:淡水对照组(T0组);从淡水直接过渡到盐度30(T30组);从淡水直接过渡至盐度14,然后以升盐速度2/d(T2组)和6/d(T6组)至盐度30。盐度驯化结果显示:99 g硬头鳟和虹鳟血清中超氧化物歧化酶(SOD)、丙二醛(MDA)和谷胱甘肽过氧化物酶(GSH-Px)活性均受盐度驯化方式、时间及其交互作用影响显著。T30组99 g虹鳟和99 g硬头鳟的SOD、GSH-Px和MDA活性在0.5、1、4、8、15和40 d这6个取样点均显著高于对照组(P<0.05)。盐度驯化40 d后,T2组99 g虹鳟SOD活性、GSH-Px活性和MDA含量与对照组无显著差异,99 g硬头鳟GSH-Px活性与对照组无显著差异,T6组99 g虹鳟和99 g硬头鳟的SOD和GSH-Px活性显著高于对照组。T2组395 g硬头鳟SOD活性、过氧化氢酶(CAT)活性和MDA含量与对照组相比无显著差异(P>0.05),而T6和T30组395 g硬头鳟血清中SOD活性、CAT活性、GSH-Px活性和MDA含量显著高于对照组(P<0.05)。研究结果表明,T2组渐变盐度方式更适合虹鳟和硬头鳟盐度驯化,395 g硬头鳟对盐度驯化的适应能力优于99 g硬头鳟。     

Broad classification of New Zealand inlets with emphasis on residence times

Thirty‐two New Zealand coastal inlets are tentatively classified into seven groups with probable similar circulation patterns, based on ratios of their physical parameters: volume, tidal compartment, entrance width, surface area, length, and average width. Eighteen of these have a predominant tidal flow, shown from their small ratio (ß < 4) of tidal compartment to total volume, and the cross‐sectional areas of their entrances are controlled by the tidal flow: Moutere, Waimea, Aotea, Whanganui, Avon‐Heathcote, Tauranga, Parengarenga, Porirua‐Pauatahanui, Kawhia, Nelson, Rangaunu, Raglan, Whangarei, Bluff, Otago, Hokianga, Manukau, and Whangaruru. The other fourteen range from long narrow sounds with probable strong vertical circulation (e.g., Pelorus Sound) to large bays with strong mean horizontal circulations (e.g., Tasman and Hawke Bays).

Examples of residence time calculated in inlets with ß > 4 are given for Tasman Bay, a bay with a mean horizontal circulation, which has a residence time of 1–3 months, and Pelorus Sound, an inlet with a vertically complex circulation, which has a residence time of about 20 d.     

Infaunal benthic communities of the New Zealand continental shelf

Seventeen infaunal benthic communities are recognised from sampling on the New Zealand continental shelf and upper continental slope. These are characterised in terms of geographic distribution, substrate and bathymetric range, and commoner infaunal bivalves and echinoderms.     

Distribution of fish in tributaries of the lower Taieri/Waipori rivers,South Island,New Zealand

The distribution of fish within eight small streams feeding into the lower Taieri and Waipori Rivers on the east coast of New Zealand's South Island was examined. A total of eight native and two introduced species were recorded. Eels (Anguilla spp.) and giant kokopu (Galaxias argenteus Gmelin) were the most widely distributed species being located in all streams surveyed. Common bullies (Gobiomorphus cotidianus McDowall) and brown trout (Salmo trutta Linnaeus) were also widely distributed being detected in seven and six of the streams respectively. Three streams of potential conservation significance were identified. These were Picnic Gully Creek which contained a significant population of banded kokopu (Galaxias fasciatus Gray), a Category C threatened species, and Cullens and Alex Creeks which contained significant populations of giant kokopu, a Category B threatened species. The population of giant kokopu in Cullens and Alex Creeks currently represents the largest known population of this species on the east coast of New Zealand (excluding Southland). It has been suggested that brown trout may influence the distribution of native galaxiids. The longitudinal distribution of giant kokopu and brown trout in streams was examined at night using a continuous spotlight sampling approach. Distributions of these two species rarely overlapped at a local scale. Coarse habitat use data indicated that brown trout regularly occupied a variety of habitat types including modified agricultural channels. In contrast, giant kokopu rarely used agricultural channels being predominantly located in pool habitats.