二氧化氯控制欧洲鳗细菌性疾病

以二氧化氯作为一种新型的消毒剂和杀菌剂 ,以传统含氯制剂 (如漂白粉、二氯异氰尿酸钠、三氯异氰尿酸 )作为对照 ,比较其在治疗欧洲鳗细菌性疾病及改善养殖水质方面的显著特性 ,其作用活性与环境 (如 p H、温度、氨及有机物 )因子的关系。结果表明 ,二氧化氯 (Cl O2 )对爱德华氏菌、嗜水气单胞菌和柱状屈挠杆菌有很好的杀灭效果 ,能迅速治愈欧洲鳗的赤鳍病和肝肾病 ,且能有效改善养殖水体 ,水中生化需氧量比对照组下降 10 %～ 30 % ,化学耗氧量下降 5% ,溶氧量提高 8%～ 10 %。当二氧化氯体积分数为 8m L/m3时 ,其杀菌率均高达 90 %以上 ,为氯制剂的 1.8倍 ,且活性不受环境因子的影响     

海水养殖中应用蛭弧菌控制病原菌的前景与问题

蛭弧菌是一类专门以捕食细菌为生的寄生性细菌，由于它具有寄生和裂解细菌的生理特性，尤其是对许多致病菌有裂解作用，越来越多的科研工作者将其视为重要的生物净化因子之一。蛭弧菌在海水养殖中用于控制病原菌，是近几年来的研究热点，蛭弧菌对大多数海产致病菌有较强的裂解能力，能改善水质，安全环保，有可能取代抗生素，成为控制病害的新手段。本文综述了蛭弧菌在海水养殖应用方面的研究情况及存在的问题。     

长江口及毗邻海域粪大肠菌和细菌总数在夏秋季的变化

2005年夏、秋季对长江口及毗邻海域(长江口、杭州湾和舟山海域)进行了粪大肠菌群和细菌总数的调查研究,并分析了近10年来的变化趋势,结果表明:夏季长江口及毗邻海域表层水体分大肠菌数平均值20个/L,仅长江口的西部海域粪大肠菌群数量在40~80个/L之间,其余海域基本未检出;秋季表层水体粪大肠菌群数量平均为101个/L,变幅范围为20~24000个/L,其中横沙一带海域存在1个数量为105个/L以上的高值区;夏季长江口及毗邻海域表层水体细菌总数平均为6.4×102cfu/mL,变幅范围为2.0×102~7.3×103cfu/mL,高值区位于舟山群岛以东海域、长江口的中北部及南部等海域,细菌总数在103cfu/mL以上;秋季表层水体细菌总数平均为4.7×103cfu/mL,明显高于夏季,变幅范围为1.3×103~1.8×104cfu/mL,高值区主要出现在长江口东南部、杭州湾西部、舟山海区的中北部等海域,细菌总数在104~105cfu/mL之间;1996—2005年期间,全海域及各海区粪大肠菌群数量在1999年均达到最高,然后逐渐下降,在1996—1999年间海域的细菌数量呈明显的上升趋势,2000年以后数量逐渐减少,估计与海域的有机污染程度下降有关。     

Antiangiogenic activity of low-temperature lysozyme from a marine bacterium <Emphasis Type="Italic">in vivo</Emphasis> and <Emphasis Type="Italic">in vitro</Emphasis>

We extracted marine low-temperature lysozyme (MLTL), a novel lysozyme, from a marine microorganism through fermentation. Our previous study suggested that a low molecular weight (16 kDa) may exert anti-tumor activity through antiangiogenesis. In this study, we extracted a high weight (39 kDa) and investigated its antiangiogenic activity in vivo and in vitro. Using zebrafish embryos as an in vivo study model, we found that treatment with MLTL significantly inhibited the growth of subintestinal vessels (SIVs) in a dose-dependent manner and that 400 μg/ml MLTL was sufficient to block the growth of SIVs. An in vitro study conducted using human umbilical vein endothelial cells (HUVECs) revealed that MLTL suppressed the proliferation, migration and tube formation of HUVECs in a dose-dependent manner. Interestingly, assays by flow cytometry and DNA electrophoresis indicated that MLTL was able to induce apoptosis of HUVECs. Moreover, further study demonstrated that the disruption of intracellular Ca²⁺ homeostasis may play an important role in MLTL induced apoptosis of HUVECs. Taken together, the results of this study demonstrate for the first time that MLTL inhibits angiogenesis through its pleiotropic effects on vascular endothelial cells and induces apoptosis through regulation of cellular Ca²⁺ levels. The results of this study also revealed a possible mechanism underlying the antiangiogenic effect of MLTL and suggested that MLTL may be a promising new antiangiogenic agent for use in cancer therapy.     

铁细菌在北极特定海区沉积物中的分布

用平板法对取自北极加拿大海盆和楚科奇海10个沉积物岩芯中184个样品进行铁细菌检出率和含量分析.结果表明,4 ℃培养时铁细菌的检出率为10.33%,含量范围均为未检出～ 8.40×106 个/g(湿样,下同),平均含量为7.76×104 个/g.25 ℃培养时铁细菌的检出率为40.22%,含量范围均为未检出～1.00×108 个/g,平均含量为1.40×106 个/g.研究区沉积物中铁细菌含量高于太平洋深海沉积物中铁细菌含量;与前人在淡水沉积物中所作的调查相比,研究区的铁细菌含量落在前人调查结果范围内,但比细菌簇中铁细菌含量要低,这表明研究区海底沉积物的微生境较适合铁细菌的繁衍.对铁细菌在不同水深、经纬度以及沉积物深度中的分布进行了讨论,结果表明铁细菌检出率随沉积物深度增加而增大,含量变化呈现出浅层少、下层多之势.铁细菌的纬度分布范围较大,并显出有扩大的趋势.铁细菌含量与检出率随水深加大也呈跳跃式变化,水较深时铁细菌有较高的检出率和数量,但离陆距离的增加又会在物质供应上对其生长产生制约.培养温度对铁细菌分布有一定影响,温度提高不仅提高了铁细菌检出率,也增加了铁细菌的含量.这也许与部分铁细菌的最适宜生长温度较高有关,温度升高有利于某些铁细菌的生长发育.     

A culture-dependent survey of thermophilic bacteria from hot springs in Xiamen area in China

Microbes are believed to play important roles in ecosystem function in many environments. The hot springs of Xiamen Island are close to the Xiamen Sea, and may have some characteristics different from those of inland hot springs. Microbes living in the hot springs of Xiamen may have new characteristics. However, little is known about microbial communities of hot springs close to the Xiamen Sea. A culturedependent survey of microbial population in the Xiamen hot springs was performed by using an approach combining total cellular protein profile identification and 16S rRNA gene sequencing. A total of 328 isolates of bacteria were obtained from liquid and sediment samples from the Xiamen hot springs, including neutrophilic thermophilic bacteria and moderately thermophilic acidophiles. Neutrophilic thermophilic bacteria, which grow at a temperature range of 55—90℃ including Rhodothermus marinus (Strain 1), Thermus thermophilus (Strain 2), Thermus thiopara (Strain 3), Geobacillus stearothermophilus (Strain 4), Geobacillus thermoleovorans (Strain 5), and Pseudomonas pseudoalcaligenes (Strain 6), were recovered by 2216E plates. Moderately thermophilic acidophiles, which can grow at temperatures above 50℃ and a pH range of 1.8—3.5 such as Alicyclobacillus acidoterrestris (Strain 8), Sulfobacillus acidophilus (Strain 9), and Sulfobacillus thermosulfidooxidans (Strain 10), were isolated on selective solid medium containing sulfur and Fe2+. Among these strains, Rhodothermus marinus, Thermus thermophilus and Geobacillus stearothermophilus are not only thermophiles, but also halophiles. One bacterium strain (Strain 6) shared 99% nucleotide sequence homology with Pseudomonas pseudoalcaligenes on the 16S rRNA gene sequence, but was quite different from Pseudomonas pseudoalcaligenes in biological characteristics, suggesting that it may represent a novel thermophilic species. Results indicated that various species of neutrophilic thermophiles and moderately thermophilic acidophiles were widely distributed in the Xiamen hot springs and that Rhodothermus marinus and Thermus thermophilus dominated the cultivable microbial community.     

Growth of marine bacteria and ammonium regeneration from substrates in different C: N ratios

Natural assemblages of marine bacteria were chosen in a batch culture experiments. The impact of varying nitrogen substrate concentrations and the substrate C:N ratios (C:N_S) on the bacterial C:N ratio (C:N_B), the bacterial growth efficiency (BGE) and ammonium regeneration was mainly examined. The C:N_S ratios varied from 5:1 (carbon limitation) to 40:1 (nitrogen limitation) with varying combinations of glucose and NO₃^-. The C:N_B ratio had positive relationship with the C:N_S ratio (r=0.93, n=8), whose value was 3.77 when the C:N_S ratio was 5:1 but increased to 6.47 when the C:NS ratio was 40:1. These results indicate that the C:N_B ratio is a potential diagnostic tool for determining the bacterial growth in natural waters controlled by either, carbon or nitrogen. BGE decreased with the declining nitrate concentration and negatively related to C:N_s (r=-0.51, n=8). The average value of BGE was 0.20. This value was a little lower than other reports, which could be induced by the nitrogen source used in our experiments. Finally, regeneration time of ammonium delayed with the increasing C:N_S ratio, which indicates that there were different metabolism mechanisms when bacterial growth was limited by carbon source and nitrogen source.     

Isolation and identification of bacteria associated with the surfaces of several algal species

We conducted this study to assess the diversity of bacteria associated with the surfaces of algae based on 16S rDNA sequence analyses.Twelve strains of bacteria were obtained from the surfaces of the following four species of algae:Gracilaria textorii,Ulva pertusa,Laminaria japonica,and Polysiphonia urceolata.The isolated strains of bacteria can be divided into two groups:Halomonas and Vibrio,in physiology,biochemical characteristics and 16S rDNA sequence analyses.The phylogenetic tree constructed based on ...