三株赤潮硅藻5.8SrDNA及转录间隔区(ITS)的克隆及序列分析

对引发赤潮的3株硅藻——1株尖刺拟菱形藻(Pseudo-nitzshia pungens)和2株中肋骨条藻(Skeletonema costatum)的5.8SrDNA和ITS(internal transcribed spacers)序列进行了PCR扩增、克隆和序列测定,并分析了硅藻门10株赤潮藻(7株从GenBank获得)的系统进化关系.研究结果表明,尖刺拟菱形藻的ITS和5.8SrDNA的长度为693bp,SK-1(分离自东海赤潮暴发区)测序得到715bp,除ITS和5.8SrDNA外,还包含部分18SrDNA和28SrDNA;SK-2(分离自青岛养殖场)的ITS和5.8SrDNA的长度为331bp,尖刺拟菱形藻与从GenBank中获得的2株尖刺拟菱形藻相似程度最高,为100%,与该属的多列拟菱形藻相似程度稍低,为82.9%.SK-2的ITS序列与SK-1的相似程度很低,只有51%,但与拟中肋骨条藻的ITS序列相似程度高,为95.5%.SK-1的ITS序列与拟中肋骨条藻的相似程度也低,为56.7%.系统进化树反映的结果与相似性反映的结果一致.研究的该株尖刺拟菱形藻从根据ITS序列研究的结果与形态鉴定的结果看是一致的;SK-2可能属于拟中肋骨条藻;SK-1比较特殊,有待于用其他的方法进一步研究确定其分类地位.     

Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences

The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellae and N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337 bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher than that of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% tol 1.73%. In addition, UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other. Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether they belong to a single species or not are still undefined, and the more genes of them should be further investigated, in combination with the systematical and detailed morphological study.     

无人机载线阵摆扫CCD影像几何校正

针对无人机载线阵CCD传感器摆扫成像的技术特点,利用GPS/INS组合导航系统提供的导航遥测数据,对无人机载的线阵摆扫CCD影像建立了严格的成像模型,并设计了详细的几何校正流程。实验结果证实采用文中的成像模型和几何校正流程,可获得正确、高精度地理编码的线阵摆扫CCD正射影像。     

基于Google Earth影像图遥感解译在我国西北矿产地质调查中的应用

在新疆扎河坝煤矿、买乌开、科克库都克三幅1∶50000区域地质矿产调查当中,笔者利用Google Earth(GE)图像数据进行了遥感地质解译,获得了满意的效果。在本次遥感解译工作中,所获得的GE卫星影像的清晰度远远大于以往的ETM多波段合成图像。其包含的可解译地质信息也比以往的卫星影像更加丰富。通过对GE影像进行数据采集、完美镶嵌、校正成为1∶5万标准图幅,使其坐标误差控制在30 m以内,并建立了测区内各地质构造单元的解译标志。良好的GE影像图的解译资料推动了野外工作的顺利进行。     

利用航天数据制图在国外铁路项目中的应用

本文详细介绍了在国外铁路项目前期工作实践中,采用影像纠正、自动提取等高线、影像矢量化等技术,摸索出的一套新的制图方法.     

GIS新引擎——“真图”数据解决方案

提出了一种基于移动测量系统技术的"真图"数据解决方案。一方面极大地丰富了GIS数据源,另一方面通过相应的真图影像数据管理软件及开发包扩展GIS功能,为各行业应用提供了地理信息数据解决方案。     

谈谈卫星遥感影像数据在土地调查应用——以杭州市区2007年度为例

一、概况 1、项目背景 杭州市区（不合萧山、余杭）土地利用现状数据为2006年调查数据,距2007年已有一年多,由于杭州市区变化频繁,土地利用变化情况复杂,为进一步提高土地调查成果质量,做到数据、图件和实地“三者一致”,为政府快速提供真实、准确的土地利用现状数据,杭州市国土资源局决定采用高分辨率卫星遥感影像数据作为2007年度土地变更调查工作的基础数据,通过对高分辨率卫星影像的识别、判读实现准确并快速地提取出土地变更信息,更为精确地勾绘出变化图斑的边界线,外业核查时更为明晰、高效。     

Main Controlling Factors on Hydrocarbon Accumulation and Distribution in Marine Sedimentary Sequences in South China

Multiple source rock assemblages were deposited in the sedimentary provinces in South China in geologic history,and some of them were destructed by and some survived against multiple tectonic movements.Therefore,multiple sources,mixed sources,and uneven distribution of sources occurred in the marine sedimentary basins in South China during the late stage of hydrocarbon pooling.Epidiagenesis of the marine carbonate reservoirs and its modification to reservoir poroperm characteristics determined the formation and the scale of natural gas pools.The exploration practices show that the large to medium gas fields mainly occur in areas with high-quality reservoirs.Detailed study of the paleo-oil accumulations and typical oil and gas reservoirs reveals that the basins experienced multiphase superimposition and modification,leading to the distribution of the Paleozoic paleo-oil accumulations and bitumen in the peripheral areas.The phenomenon that oil and gas production concentrates in the Sichuan basin indicates that the overall sealing conditions of a basin determine the oil/gas potentials and the scale of oil and gas production.This is a critical factor controlling the accumulation and distribution of gas in the marine sequences in South China.The early oil and gas pools in the Yangtze platform left billions of bitumen in the peripheral areas due to the destruction of seals.Since the Himalayan,"late-generation and late-accumulation" gas pools represented by the gas pools in the Sichuan (四川) basin were formed in the marine sedimentary sequences in South China as a result of the change of the sealing conditions.Current gas discoveries appear to be "paleo-generation and paleo-accumulation" gas pools but actually are "late-generation and late-accumulation" gas pools.These patterns of hydrocarbon pooling clearly depict themselves in western Sichuan basin and Weiyuan (威远)gas field.It is revealed that the gas pools in the Sichuan basin were mainly formed as a result of hydrocarbon phase change (thermal cracking of oil to gas),miscible migration,and dynamic equilibration since the Himalayan.A large number of gas pools were formed in the Himalayan and the gas pools in the marine sequences are characterized by late pooling; this kind of gas fields/pools are controlled by:(1) effectiveness of modification and superimposition of the marine basins,(2) effectiveness of the source rocks,(3) effectiveness of the overall preservation conditions,and (4) effectiveness of plays.     

BP神经网络模型在澜沧江流域径流量模拟中的应用

河川径流等水文时间序列属于复杂的非线性系统,使用回归分析等传统的分析方法,难以获取和描述其内在关联和变化规律。利用现有的相关站点的径流量历史数据和输沙量、降水量数据,在进行规格化处理和主成分分析的基础上,利用三层BP人工神经网络模型,对澜沧江流域上游昌都站径流量与各关联因子之间复杂的非线性映射关系进行模拟,采用拟牛顿算法对模型进行训练,模拟结果达到期望精度要求,并利用1982年~1985年实测数据进行模型验证。结果证明利用BP神经网络模型对澜沧江流域站点的月径流量序列进行模拟、预测和数据补缺处理具有可行性。