扇贝碱性磷酸酶中Zn的作用

以华贵栉孔扇贝Chlamys nobilis(Reeve)为材料,获得聚丙烯酰胺凝胶电泳为单一蛋白区带的酶制剂。用凝胶过滤法测得该酶的分子量为159000,由20种氨基酸组成,共1118个氨基酸残基。每个酶蛋白含有4个Zn原子。将该酶的酶制剂制成脱Zn酶后,与相同浓度过度性两价金属离子作用,其活力回升以Zn最强,Co次之。若向脱Zn酶蛋白分别加入一定量Zn原子或Co原子,根据实验结果初步推测:每个酶蛋白的4个Zn原子中有2个Zn原子先与该酶结合,与酶的催化作用有密切关系;另外2个Zn原子与酶结合,则起维持酶结构的作用,与哺乳动物和Eschericha coli来源的碱性磷酸酶研究结果一致。此外,本实验采用可见吸收光谱和圆二色谱(CD)来确证Co能很好地取代该酶蛋白内的Zn,与Simpon报道相比较,得知当Co原子加入脱Zn酶时,先加2个Co~(2 )离子结合到该酶的结构部位,而后加2个Co~(2 )离子才结合到催化部位。     

含环向表面裂纹管道功率流特性与裂纹识别

研究在外力激励下含有环向表面裂纹损伤的壳式管道的波传播与功率流特性,并给出裂纹识别方法。管道的振动用Flügge方程来描述,通过将裂纹模拟为分布的线性弹簧,运用断裂力学理论,得到裂纹区域的局部柔度矩阵,从而建立外力作用处以及裂纹两侧区域的位移和内力的连续性条件对裂纹管道的功率流进行求解。讨论功率流特性与裂纹位置和管道特性等参数的关系,最后给出了裂纹识别的方法。     

Second-Order Analytic Solutions of Nonlinear Interactions of Edge Waves on A Plane Sloping Bottom

Based on the full water-wave equation,a second-order analytic solution for nonlinear interaction of short edge waves on a plane sloping bottom is presented in this paper.For special case of slope angle β=π/2,this solution can reduced to the same order solution of deep water gravity surface waves traveling along parallel coastline.Interactions between two edge waves including progressive,standing and partially reflected standing waves are also discussed.The unified analytic expressions with transfer functions for kinematic-dynamic elements of edge waves are also given.The random model of the unified wave motion processes for linear and nonlinear irregular edge waves is formulated,and the corresponding theoretical autocorrelation and spectral density functions of the first and the second orders are derived.The boundary conditions for the determination of the parameters of short edge wave are suggested,that may be seen as one special simple edge wave excitation mechanism and an extension to the sea wave refraction theory.Finally some computation results are demonstrated.     

Damping modification factors for SDOF systems subjected to near‐fault,far‐fault and artificial earthquakes

Damping modification factors (DMF) are used in modern seismic codes to adjust elastic response spectral values corresponding to 5% of viscous damping to other higher or lower damping levels. This paper presents a simple and effective procedure to estimate DMF for single‐degree‐of‐freedom systems. Empirical expressions are proposed for displacement, velocity and acceleration response spectra, where four types of soil conditions, from hard rock to soft soil are considered. This study also examines, for the first time, the influence of artificial earthquakes on DMF. Furthermore, natural near‐fault and far‐fault seismic ground motions are considered where it is testified that the fault distance has no impact on DMF. Finally, it confirms that, in contrast to the considerations of many seismic codes, DMF are strongly dependent on the period of structural vibration while there are significant problems of using the same modification factor to estimate maximum displacement, velocity and seismic forces. Copyright © 2010 John Wiley & Sons, Ltd.     

对ENVISAT ASAR level2算法固有误差的分析

欧洲空间局的ENVISAT ASAR level 2算法是从合成孔径雷达(SAR)单视复图像反演涌浪方向谱的算法.该算法假设双峰海浪谱的SAR图像交叉谱是涌浪的图像交叉谱和风浪的图像交叉谱之和.实际上双峰海浪谱的SAR图像交叉谱中还有一个混合项,正是该混合项导致ENVI-SAT ASAR level 2算法有固有误差.利用遥感仿真的方法分析了不同海况条件下该算法的这一固有误差,结果表明,只有在有效波高较小、或风浪的成分较少、或双峰海浪的传播方向较靠近SAR距离向、或波长较长时固有误差才较小,ENVISAT ASAR level 2算法对海浪谱的反演才较为适用.     

Simulation of Random Waves and Associated Laminar Bottom Shear Stresses

This work presents a new approach for simulating the random waves in viscous fluids and the associated bottom shear stresses. By generating the incident random waves in a numerical wave flume and solving the unsteady two-dimensional Navier-Stokes equations and the fully nonlinear free surface boundaiy conditions for the fluid flows in the flume, the viscous flows and laminar bottom shear stresses induced by random waves axe determined. The deterministic spectral amplitude method implemented by use of the fast Fourier transform algorithm was adopted to generate the incident random waves. The accuracy of the numerical scheme is confirmed by comparing the predicted wave spectrum with the target spectrum and by comparing the nanlerical transfer function between the shear stress and the surface elevation with the theoretical transfer function. The maximum bottom shear stress caused by random waves, computed by this wave model, is compared with that obtained by Myrhaug＇ s model （1995）. The transfer function method is also employed to determine the maximum shear stress, and is proved accurate.     

Bubble Size Distribution for Waves Propagating over A Submerged Breakwater

Experiments are carried out to study the characteristics of active bubbles entrained by breaking waves as these propagate over an abruptly topographical change or a submerged breakwater. Underwater sounds generated by the entrained air bubbles are detected by a hydrophone connected to a charge amplifier and a data acquisition system. The size distribution of the bubbles is then determined inversely from the received sound frequencies. The sound signals are converted from time domain to time-frequency domain by applying Gabor transform. The number of bubbles with different sizes are counted from the signal peaks in the time-frequency domain. The characteristics of the bubbles are in terms of bubble size spectra, which account for the variation in bubble probability density related to the bubble radius r. The experimental data demonstrate that the bubble probability density function shows a - 2.39 power-law sealing with radius for r 〉 0. 8 mm, and a- 1.11 power law for r 〈0.8 mm.     

基于ASTER 和ETM+数据的蚀变信息提取 比较研究———以西藏泽当矿田为例

利用ASTER和ETM+数据分别对西藏泽当矿田试验区进行了遥感数据矿物蚀变信息提取。根据研究区岩石矿物光谱特征,采用主成分分析法,提取了含三价Fe离子和粘土矿化蚀变信息,通过对两者提取的结果进行比较之后认为,ASTER数据比ETM+数据在矿化蚀变信息提取中能进一步识别不同类型的含羟基蚀变矿物,并且也能很好的提取含三价Fe离子蚀变信息,未来具有较大的优势。     

Spectral characteristics of the nearshore waves off Paradip,India during monsoon and extreme events

Spectral and statistical wave parameters obtained from the measured time series wave data off Paradip, east coast of India during May 1996–January 1997 were analysed along with MIKE 21 spectral wave model (SW) results. Statistical wave parameters and directional wave energy spectra distinctly separate out the wave conditions that prevailed off Paradip in the monsoon, fair weather and extreme weather events during the above period. Frequency-energy spectra during extreme events are single peaked, and the maximum energy distribution is in a narrow frequency band with an average directional spreading of 20°. Spectra for other seasons are multi-peaked, and energy is distributed over a wide range of frequencies and directions. The NCEP re-analysis winds were used in the model, and the results clearly bring out the wave features during depressions. The simulated wave parameters reasonably show good match with the measurements. For example, the correlation coefficient between the measured and modelled significant wave height is 0.87 and the bias −0.25.