一种酪氨酸酶基因在笠贝初生壳形成中的表达分析

软体动物初生壳形成是贝壳早期发育研究的重要内容。目前,初生壳形成的机制尚不清晰。本文从笠贝(Lottia goshimai)中克隆了一个酪氨酸酶基因(lgo-tyr1),聚类分析表明其与长牡蛎基因cgi-tyr1是直系同源基因。Lgo-TYR1的序列分析显示它具有两个典型的铜离子结合结构域和一个信号肽。通过整装原位杂交和电子扫描显微镜观察,检测到lgo-tyr1在担轮幼虫的圆形贝壳区以及紧邻的环状细胞带中表达,表明lgo-tyr1可能参与了担轮幼虫初生贝壳的生物合成以及贝壳区边界的确立。     

The bivalve Lembulus bicuspidatus may enhance denitrification in shelf sediment at the Angola–Benguela Frontal Zone

We collected living individuals of the bivalve Lembulus bicuspidatus, which shows an unusual preference for the oxygen-deficient habitat found at the Angola–Benguela Frontal Zone of the southeastern Atlantic. With a series of incubation experiments with ¹⁵N-labelled nitrate as a tracer in combination with membrane-inlet mass spectrometry, we studied the potential contribution of L. bicuspidatus to nitrate reduction in the upper sediment layer. Our preliminary results suggest that L. bicuspidatus enhances nitrate reduction if the oxygen concentration is sufficiently low. The Lembulus-mediated nitrate reduction rate is then similar to the rate of microbial nitrate reduction in the surrounding sediment.     

Bearing capacity of offshore umbrella suction anchor foundation in silty soil under varying loading modes

Considering the current disadvantages of present offshore wind turbine foundations, a novel anchor foundation with skirt and branches is proposed, called offshore umbrella suction anchor foundation (USAF). A series of experiments and numerical simulations were performed to explore the bearing capacity of the USAF under various kinds of loading modes. The bearing characteristics and the anchor–soil interactions are described in detail for horizontal static loading, horizontal cyclic loading, and an antidrawing (pullout) test in silty soil. In the static loading test, the load–deflection of the anchor under step loading was analyzed and the normalized curve of the load–deflection was obtained to determine the ultimate horizontal bearing capacity of the anchor under normal working conditions. Under horizontal cyclic loading, the relationship between the plastic cumulative deformation and cyclic number was determined. In addition, the responses of USAF were investigated for a low wave frequency and storm surges. In the drawing test, it was found that a “segmentation phenomenon” occurred during the test. Moreover, a method to identify the maximum antidrawing load of USAF was provided based on dynamic mechanics. The numerical results show that the use of anchor branches and skirt can enhance the bearing performance of USAF to a certain degree. However, the anchor branch has a slight positive influence on the bearing performance improvement. The USAF is not only similar to a stiff short pile, but a rotation occurs. The failure envelope under composite loading (V-M) was obtained and the changes associated with changes in the aspect ratio of the internal compartment were clarified.     

深水铺管船托管架疲劳分析及动态监测点选取研究

作为S型铺管作业的关键性装备,托管架长期承受着交变载荷的作用,随着海洋开发向超深水发展,结构疲劳破坏问题不容忽视。采用疲劳谱分析的方法并结合线性累积损伤理论,对托管架结构频域下的变形进行了分析,计算了正常海况下和极端海况下托管架疲劳损伤度并对疲劳寿命进行了评估。研究发现托管架在正常海况下作业符合安全要求,在极端海况下局部结构会受到破坏。并从托管架结构安全监测角度,筛选了结构疲劳分析关键点位,为监测点位的选取提供了依据。     

Changes in hillslope hydrology in a perched,shallow soil system due to clearcutting and residual biomass removal

Sustainable fuels legislation and volatility in energy prices have put additional pressures on the forestry sector to intensify the harvesting of biomass for “advanced biofuel” production. To better understand how residual biomass removal after harvest affects forest hydrology in relatively low slope terrain, a Before-After-Control-Impact (BACI) study was conducted in the USDA Forest Service's Marcell Experimental Forest, Minnesota, USA. Hydrological measurements were made from 2010–2013 on a forested hillslope that was divided into three treatment blocks, where one block was harvested and residual biomass removed (Biomass Removed), the second was harvested and residual biomass left (Biomass Left), and the last block was left as an Unharvested Control. The pre-harvest period (2 years) was 2010–11 and post-harvest (2 years) was 2012–13. Water table elevation at the upslope and downslope position, subsurface runoff, and soil moisture were measured between May–November. Mixed effect statistical models were used to compare both the before-after and “control” treatment ratios (ratios between harvested hillslopes and the Unharvested Control hillslope). Subsurface runoff significantly increased (p < .05) at both harvested hillslopes but to a greater degree on the Biomass Left hillslope. Greater subsurface runoff volumes at both harvested hillslopes were driven by substantial increases during fall, with additional significant increases during summer on the Biomass Left hillslope. The hydrological connectivity, inferred from event runoff ratios, increased due to harvesting at both hillslopes but only significantly on the Biomass Left hillslope. The winter harvest minimized soil disturbance, resulting in no change to the effective hydraulic conductivity distribution with depth. Thus, the observed hydrological changes were driven by increased effective precipitation and decreased evapotranspiration, increasing the duration that both harvested hillslopes were hydrologically active. The harvesting of residual biomass appears to lessen hydrological connectivity relative to leaving residual biomass on the hillslope, potentially decreasing downstream hydrological impacts of similar forestry operations.     

西部省会城市新型城镇化水平综合测度研究

新型城镇化是中国经济社会健康、稳定、可持续发展的根本途径，也是消除中国城乡社会经 济“二元结构”的根本出路。根据新型城镇化的内涵，从人口、经济发展、生活质量、基础设施、资源 环境、城乡统筹 6 个方面，构建了包含 24 项指标的新型城镇化水平综合测度指标体系，运用熵值 法，对西部 11 个省会城市 2005—2015 年的城镇化发展水平进行综合测度。研究表明：西部省会城 市的新型城镇化发展水平存在显著差异，总体上可分为“高等水平”、“中等水平”、“低等水平”3 个 级别，基础设施建设是造成差异的主要驱动力。各城市不同年间的城镇化单项水平影响因素呈现 多元化，在今后的发展中应有所侧重，同时兼顾城镇化质量，推动城乡协调发展。     

环塔里木盆地绿洲城市发展与水环境质量协调度

解决好新疆环塔里木盆地绿洲城市发展与水环境质量之间的关系，对"一带一路"倡议顺利实施及新疆社会稳定具有十分重要的作用。基于2004-2017年环塔里木盆地绿洲城市发展与水环境变化状况，构建城市发展与水环境质量的综合评价模型及二者的协调度模型，以探讨环塔里木盆地绿洲城市发展、水环境质量变化及二者的协调关系。结果表明：2004-2017年，（1）环塔里木盆地绿洲城市发展综合指数呈现出逐年稳定上升的趋势，经济发展和城镇空间规模扩大是环塔里木盆地绿洲城市发展的主要形式；（2）环塔里木盆地绿洲城市的水环境质量综合指数呈现出逐年降低的趋势，水环境质量形势严峻；（3）环塔里木盆地绿洲城市发展-水环境质量系统的协调度呈现倒U型的稳定发展趋势，但总体上两者的协调度较低，城市发展和水环境质量之间的矛盾日益突出。     

利用短期静态PPP结果反演海潮负荷位移

采用香港11个GPS测站的观测资料进行1 h、2 h、3 h和4h静态PPP解算，获得4组PPP坐标序列，利用调和分析求取11个测站处8个主要分潮的负荷位移参数（振幅和相位），将其与海潮模型计算的负荷位移参数进行对比，并比较分析PPP反演值与海潮模型值改正海潮负荷信号的效果。结果表明，垂直和水平方向上，不同PPP结果反演8个分潮的负荷位移分别具有约5 mm和7 mm的差异；PPP反演8个分潮垂向负荷位移优于全球海潮模型，但水平方向上的反演效果稍弱。     

Solution for a plane strain rough‐walled hydraulic fracture driven by turbulent fluid through impermeable rock

The impact of turbulent flow on plane strain fluid‐driven crack propagation is an important but still poorly understood consideration in hydraulic fracture modeling. The changes that hydraulic fracturing has experienced over the past decade, especially in the area of fracturing fluids, have played a major role in the transition of the typical fluid regime from laminar to turbulent flow. Motivated by the increasing preponderance of high‐rate, water‐driven hydraulic fractures with high Reynolds number, we present a semianalytical solution for the propagation of a plane strain hydraulic fracture driven by a turbulent fluid in an impermeable formation. The formulation uses a power law relationship between the Darcy‐Weisbach friction factor and the scale of the fracture roughness, where one specific manifestation of this generalized friction factor is the classical Gauckler‐Manning‐Strickler approximation for turbulent flow in a rough‐walled channel. Conservation of mass, elasticity, and crack propagation are also solved simultaneously. We obtain a semianalytical solution using an orthogonal polynomial series. An approximate closed‐form solution is enabled by a choice of orthogonal polynomials embedding the near‐tip asymptotic behavior and thus giving very rapid convergence; a precise solution is obtained with 2 terms of the series. By comparison with numerical simulations, we show that the transition region between the laminar and turbulent regimes can be relatively small so that full solutions can often be well approximated by either a fully laminar or fully turbulent solution.