In vivo neutralization assays by monoclonal antibodies against white spot syndrome virus in crayfish(Cambarus proclarkii)

The neutralizing activities of eight monoclonal antibodies （MAbs） against white spot syndrome virus （WSSV） （2D2, 2B2,1D2, 1D5, 1C2, 4A1, 6A4 and 6B4） were analyzed by in vivo experiments. Gills from WSSV-infected shrimp were homogenized and ten-fold serially diluted by PBS, and then incubated with MAbs （hybridoma culture supernatant）, respectively. The mixture of WSSV and MAbs were injected into crayfish （Procambarus clarkii）. After challenge, the death rates of crayfish were counted to determine the neutralizing activities of MAbs. At the same time, the mixture of myeloma culture supernatant and WSSV or PBS was served as positive or negative control, respectively. The results showed that, at each virus dilution, the mean time to death of the crayfish injected with MAb-treated virus was significantly longer than that in the positive control, though they all showed 100% mortality within 25 d, and meanwhile, few crayfish died in the negative control. Among the eight MAbs, 2D2, 2B2, 1D2 and 1D5, especially the former two, delayed the mortality significantly, and 1 C2, 4A1 and 6A4 delayed the mortality as well but not so efficiently, while MAb 6IM was efficient only when the virus concentration increased. The results indicated that the anti-WSSV MAbs can neutralize WSSV in different virus dilutions.     

轨道平顺性与测量精度

从轨道平顺性验收标准性切入，提出轨道平顺性对控制网的精度要求；指出轨道平顺性与控制点间的相对精度相关，与控制测量网垂直于线路的的横向误差及高程相关。     

Distribution characteristics of heavy metals in surface sediments and core sediments of the Shenzhen Bay in Guangdong Province, China

The concentration of zinc, chromium, cadmium, nickel, lead and copper was measured on both surface sediments and core sediments in the Shenzhen Bay. The average contents of all metals have exceeded the background values in the coastal zone of Guangdong Province. Cadmium is a heavy pollution matter which indicates a strong pollution level according to the ecological risk coefficient method. The ecological risk index of the whole area is 299 which belongs to middle and strong level. The area with strong pollution level is the near shore area closing to the inside of Shenzhen Bay, Shakou area is in the middle level while the weak pollution level occurs in Shenzhenhe Estuary according to ecological risk index. The four sediment cores can be divided into two parts based on lead-210 dating, sediments size analysis and economic development period in surrounding areas since 1950 with strong human intervention. The sediment size, deposition rate and human activities have positive relationship with the contents of heavy metals which directly impact the pollution level of heavy metals in sediments in Shenzhen Bay.     

济南张夏组灰岩岩溶地下水供水潜力探讨

济南南部广布巨厚张夏组灰岩,其中赋存优质丰富的岩溶地下水。出露众多泉水,其流量为800～8000m3/d。该文通过简述济南地区张夏组灰岩岩溶地下水的赋存条件与水文地质特征,概略计算与评价其资源量,论证开发利用这一新水源的可行性,以期为济南供水与保泉开辟新途径。     

离散位场数据格网化方法探讨

针对均衡异常数据,比较分析了连续曲率张力样条方法、最小曲率方法和Shepard方法,结果表明张力样条方法解的全局效果较好。     

低端掌上机在控制测量中的前景分析

论述了开发低端电子手簿的可行性，介绍了HP200LX掌上机的基本特点，实用汉字库的建立原理和使用，软件界面设计的基本原则和具体做法，以及手簿的功能和特点，提出了中文电子手簿设计及开发的思路。     

利用南极走航观测评估卫星遥感海表面温度

利用1989-2005年间南极走航观测的海表面温度,对目前3个主要的卫星反演的SST产品AVHRR(Advanced Very High Resolution Radiometer),TMI(TRMM Microwave Imager)和AMSR-E(Advanced Microwave Scanning Radiometer for the Earth Observing System)进行了较为系统的评估,并着重检验了它们在南大洋的准确性.结果表明,AVHRR SST比观测数据偏冷,白天的偏差为-0.12℃,夜晚的偏差为-0.04℃,而且南大洋的冷偏差更为显著.TMI SST比观测数据明显偏暖,白天的偏差为0.48℃,夜晚的偏差为0.57℃,其温差ΔT受37GHz风速影响,在强风速(>6m/s)下这种影响仍然存在.AMSR-ESST比观测数据偏暖,白天的偏差为0.34℃,夜晚的偏差为0.27℃,而且南大洋的暖偏差相对较大.AMSR-E SST温差受水汽影响,并在南大洋随着水汽的增加而增加.通过进一步比较微波(AMSR-E和TMI)和红外(AVHRR)遥感的SST在2004年北半球冬季(即南半球夏季)的差别,发现微波遥感在热带(15°S-15°N)和南大洋区域(45°S以南)比红外遥感偏暖,而且在南大洋区域的偏差相对较大,相反在北半球中纬度区域(15°~40°N)偏冷.AMSR-E与AVHRR SST的温差,从白天到夜晚有减小的趋势,而TMI与AVHRR SST的温差无明显的变化.     

Size-fractionated uranium isotopes in surface waters in the Jiulong Estuary in China

Surface water was collected from the Jiulong Estuary for determination of activity concentrations of uranium isotopes in different size fractions, namely, greater than 53, 10 -53, 2 - 10, 0.4 -2 μm, 10 000 u -0.4 μm and less than 10 000 u fractions by microfihration and cross-flow uhrafiltration technologies. Results indicated that most of the dissolved uranium （ 〈 0.4 μm） exis- ted in the low molecular mass fraction （ 〈 10 000 u）, and the colloidal uranium-238 （10 000 u -0.4 μm） only contributed less than 1% of the dissolved uranium-238. The fractions of colloidal uranium in the dissolved phases decreased with the increasing sa- linity. A positive linear relationship between uranium-238 activities and salinities was observed for the dissolved, colloidal and low molecular mass fractions, indicating a conservative behavior of uranium in the Jiulong Estuary. In the particulate phases （ 〉 0.4 μm）, the partitioning of uranium isotopes among different size fractions was controlled by the partitioning of particle concentrations. In the regions with salinities below 20, the partitioning of uranium-238 among different size fractions was as follows： 10 - 53 μm 〉 2 - 10 μm 〉 0.4 - 2 μm greater than above 53 μm. However, the order at the offshore station with salinities above 30 changed as follows ： 0.4 - 2 μm 〉 10 - 53 μm 〉 2 - 10 μm greater than above 53 μm. The fraction of the 0.4 - 2 μm particles increased at the offshore station, suggesting the increased contribution of the authigenic uranium. The activity ratio of uranium-234 to uranium-238 in the dissolved phases, including the low molecular mass fraction and the colloidal fraction, was larger than unity, showing the occurrence of excess uranium-234. In contrast, the activity ratio of uranium-234 to uranium-238 in all size fractions of the particulate phase was close to the equilibrium value （1.0）. The observed different values of the activity ratio of uranium-234 to uranium-238 in the dissolved phase and the partic     

藏、汉对比视角下的城市空间环境认知研究——以中国西藏日喀则市为例

以分布式认知理论为基础,采用质性研究法、数量分析法以及GIS空间分析技术,探讨日喀则市藏族、汉族居民对城市空间环境认知的异同性。研究表明,藏族对城市空间环境的认知得分高于汉族对城市空间环境的认知得分。藏族、汉族对城市空间环境的认知水平可分为高、中、低3类。认知空间呈现圈层结构与混合结构模式,可分为藏汉一致性、藏族独立以及汉族独立3种认知空间类型。影响居民对城市空间环境的认知因素有共性因素和差异性因素。景观走廊质量、宜居性、基础设施两项是藏族居民关注的主要影响因素,汉族居民更关注公共服务设施的服务能力以及对当地文化的融入感。     

论船舶物权变动登记之公信力

船舶物权变动采用登记公示方式,登记应当具有公信力,登记公信力制度的确立旨在保护第三人之利益。登记是制度创设,应该承认船舶登记的公信力以满足该制度之宗旨。船舶登记的对抗效力不影响登记公信力的存在,但该登记的公信力受到限制。