CryoSat-2卫星海冰区域波形识别及海冰干舷高确定

利用40%阈值法对CryoSat-2卫星波形数据进行重跟踪,将波形特征参数和海冰浓度相结合,对海冰和Lead(浮冰之间的开阔水域)进行有效识别。利用沿轨前后搜索算法计算海冰干舷高,并引用AWI结果,绘制2011~2013年北冰洋多年冰区域和一年冰区域平均海冰干舷高变化趋势图。比较本文结果与AWI结果的各年同期数据,验证本文结果的可靠性。     

Estimating the Turbulence Characteristics in the Bottom Boundary Layer of Monterey Canyon

From April 24 to October 25, 2011, an Acoustic Doppler Velocimeter(ADV) continually running for 185 d was mounted on the smooth ridge at the edge of Monterey Canyon to observe turbulence in the bottom boundary layer. The ADV was set at 1.4 m above the bed bottom, continuously run for 1 min with a 2-minute interval with sampling frequency 64 Hz. The long-time continual observation is significant to reveal variations of turbulent characteristics and show some differences from the classic traditional turbulent theory. Eliminating the noise by the ‘Phase-Space Thresholding Method', rotating the coordinate and low-pass filtering the velocity were applied for data processing. This paper was mainly to estimate the turbulent kinetic energy dissipation rate by the inertial dissipation method, friction velocity, drag coefficient and significant periods of the turbulent characteristics with the ADV data. The results show that there is a strong, rotating bottom flow up to 0.398 m s-1 with predominantly semidiurnal period and less significantly diurnal and semilunar period. The turbulent kinetic energy dissipation rate ranges from 1.09×10-8 W kg-1 to 6.62×10-5 Wkg-1, which can vary with 2 or 3 orders of magnitude in one day. The daily averaged variations of friction velocity and drag coefficient are 6.50×10-3–2.32×10-2 m s-1 and 6.30×10-3–4.36×10-2, respectively. All the characteristics have a remarkable semidiurnal period. In the bottom boundary layer with a rotating tide, the parameterized coefficients to describe ε-u* and ε-Et relationships are much smaller than the traditional value.     

Research of Marine Sensor Web Based on SOA and EDA

A great deal of ocean sensor observation data exists, for a wide range of marine disciplines, derived from in situ and remote observing platforms, in real-time, near-real-time and delayed mode. Ocean monitoring is routinely completed using sensors and instruments. Standardization is the key requirement for exchanging information about ocean sensors and sensor data and for comparing and combining information from different sensor networks. One or more sensors are often physically integrated into a single ocean ‘instrument' device, which often brings in many challenges related to diverse sensor data formats, parameters units, different spatiotemporal resolution, application domains, data quality and sensors protocols. To face these challenges requires the standardization efforts aiming at facilitating the so-called Sensor Web, which making it easy to provide public access to sensor data and metadata information. In this paper, a Marine Sensor Web, based on SOA and EDA and integrating the MBARI's PUCK protocol, IEEE 1451 and OGC SWE 2.0, is illustrated with a five-layer architecture. The Web Service layer and Event Process layer are illustrated in detail with an actual example. The demo study has demonstrated that a standard-based system can be built to access sensors and marine instruments distributed globally using common Web browsers for monitoring the environment and oceanic conditions besides marine sensor data on the Web, this framework of Marine Sensor Web can also play an important role in many other domains' information integration.     

鲤JAK2基因分子克隆及组织表达分析

采用同源克隆策略和RACE-PCR技术,克隆得到可能的鲤(Cyprinus carpio)两面神激酶2(JAK2)基因的c DNA全长序列,包括3 378 bp的开放阅读框,732 bp的5'-非编码区,529 bp的3'-非编码区,总长度达4 639bp。开放阅读框可编码1 125个氨基酸,推测分子质量和理论等电点分别为129.33 ku和6.99。同源性分析显示,克隆的鲤鱼基因与斑马鱼(Danio rerio)JAK2同源性最高,其氨基酸序列同一性和相似度分别达89%和95%。结构域预测表明,所编码氨基酸序列包含FERM、SH2及两个酪氨酸激酶结构域,这4个结构域也保守地存在于其他脊椎动物的JAK分子中。高级结构预测显示,鲤JAK2主要包括α-螺旋(α-helix)、β-折叠(β-sheet)和连接环(loop)等3类结构元件。脊椎动物JAK分子系统进化树显示,JAK1、JAK2、JAK3和TYK2等4类JAK分子分别聚类,鲤JAK2处于JAK2支系中,与所有其他的鱼类JAK2聚为一大支,并与两栖类和哺乳类组成的另一大支构成姊妹群,表明它们具有共同的祖先基因,为直系同源关系。实时荧光定量PCR检测结果表明,鲤JAK2在皮肤中表达量最高,其次是肠、血液和脑,而在肌肉、鳃、头肾、心、肝、脾中表达量较低;鲤JAK3在脾中表达量最高,在其他组织中表达量均很低,甚至未检出。     

卵形鲳鲹PPARα基因cDNA序列的克隆、组织表达及生物信息学分析

采用RACE-PCR克隆卵形鲳鲹(Trachinotus ovatus)过氧化物酶体增殖物激活受体(peroxisome proliferator activated receptors-α,PPARα)基因的c DNA序列全长,并应用生物信息学方法分析其编码蛋白质的理化性质和结构特征。结果表明:卵形鲳鲹PPARα基因(Gen Bank登录号KP893147)c DNA全长1 930 bp,开放阅读框(ORF)为1 425 bp,共编码474个氨基酸,其编码的蛋白质为不稳定蛋白,无信号肽和跨膜结构,二级结构由α螺旋、β转角、伸展片段和无规则卷曲组成,且α螺旋占较大比例;预测显示,该蛋白有PPARs基因家族典型的DNA结合区(DBD)和配体结合区(LBD);序列对比表明,卵形鲳鲹PPARα基因与尼罗罗非鱼(Oreochromis niloticus)、花鲈(Lateolabrax japonicas)、大黄鱼(Larimichthys crocea)、金头鲷(Sparus aurata)、军曹鱼(Rachycentron canadum)等有较高的同源性(81%~89%);蛋白系统进化树分析显示,在人(Homo sapiens)、鼠(Mus musculus)、鸭(Gallus gallus)、花鲈、大黄鱼、军曹鱼等动物中,卵形鲳鲹的PPARα蛋白与军曹鱼的进化关系最为密切(94%),与人(68%)、鼠(68%)、鸭(67%)等的同源性较低。荧光定量分析显示,卵形鲳鲹PPARαm RNA在脑、肾脏、肠、脾脏等组织表达水平较高,其次是皮肤、肌肉,在心脏、肝脏中表达量较低。     

基于速度场模型的区域CGCS2000坐标实时转换

从WGS84到CGCS2000的坐标转换,涉及坐标框架转换和历元转换两个问题。如何提高大区域坐标转换精度,是一直以来的研究热点,针对大区域坐标转换精度较低的问题,考虑历元转换,提出融合速度场改正信息的坐标转换方法。该方法对建立速度场改正模型进行了研究,分析比较了各模型的精度和可靠性,在此基础上选择一种模型用于实现CGCS2000坐标实时转换。实例表明,该转换方法不受区域范围的影响,精度高且误差在各方向上都分布均匀。     

基于粒子群算法优化SVM的GPS高程拟合方法

采用支撑向量机(Support Vector Machine,SVM)模型进行GPS高程拟合时,拟合精度受模型参数(核参数和惩罚因子)选择影响较大,传统交叉验证法存在计算复杂,易陷入局部最优的问题.本文将粒子群(Particle Swarm Optimization,PSO)算法引入SVM模型,利用PSO全局搜索能力强和收敛速度快的特点对SVM模型进行优化,提升GPS高程拟合精度的同时增强模型的泛化适应性.最后基于实际算例对所提方法的拟合性能进行验证,结果表明相对于传统交叉验证SVM模型,所提PSO-SVM模型能够获得更高的拟合精度,并且对复杂地势具有更强的适应能力.     

利用PCI GXL-A进行航空影像DEM匹配及编辑

随着航空摄影测量技术的快速发展,海量正射影像数据生产已经成为测绘生产的常态.而正射影像的生产离不开数字高程模型(Digital Elevation Model,DEM)数据,因此快速高效的生产DEM对正射影像的生产至关重要.本文以航空影像作为实验数据,利用影像处理软件的航空影像处理模块2018年版本(GXL 2018 SP3 Aerial)空三加密后进行高精度数字地表模型匹配,并使用软件中数字高程模型的编辑(Geomatic Focus的DEM Editing)功能对数字地面模型(Digital Terrain Models,DTM)进行快速编辑生成DEM数据,为今后大批量DEM生产及编辑工作提供借鉴.     

HERON背包SLAM激光扫描系统在建筑规划测量中的应用

目前城市建筑工程规划核验竣工测量工作仍以传统测绘为主要技术手段,随着三维激光扫描技术的发展,即时定位与地图构建(SLAM)背包激光扫描技术发展日趋迅速.通过对比分析传统竣工测绘和华测HERON背包SLAM激光扫描系统两种测绘方法,探讨HERON背包SLAM激光扫描系统在建筑竣工测量中的实用性.     

基于GIS的城市工程建设多测合一管理平台设计与实现

近年来,为了加快疏解企业办事堵点、痛点,深化"放管服"改革,优化营商环境建设,最大限度惠企便民,国内许多城市开始深化工程建设项目审批制度改革,全面开展工程建设项目多测合并、联合测绘和成果共享工作.结合东莞市工程建设项目审批的实际情况,探讨多部门协同的多测合一(联合测绘)管理流程,构建多测合一全过程管理数据库,开发东莞市工程建设多测合一管理平台,实现工程建设项目审批制度实施全流程管理,助推工程建设项目审批改革.