中国陆地净初级生产力的季节变化研究

了解不同季节陆地净初级生产力(NPP)的变化及与气候的相互关系以及在不同地类的差异对深刻理解我国陆地生态系统对全球气候变化的响应和陆地碳循环研究具有重要意义。本文使用1981～2000年间GLO-PEM模型模拟的我国陆地NPP数据和同期气温、降水以及土地利用数据, 研究不同季节我国陆地植被NPP的变化。结果表明,在1981～2000年期间,四个季节的NPP都呈显著增加趋势,春季是NPP增加速率最快的季节,夏季是NPP增加量最大的季节。耕地在春、夏和秋季NPP增长和增长率最高,林地冬季NPP增长最多而水域冬季NPP增长率最高。夏季NPP增长最高的区域分布于我国东部的多数地区、内蒙古东部、四川盆地、贵州东部、藏南和新疆西部;夏季NPP降低最多的区域分布于在呼伦贝尔高原、鄂尔多斯高原、黄土高原、青藏高原东部和新疆西北部。     

海洋生态系统净生产力研究进展

海洋生态系统净生产力 （net ecosystem production，NEP） 表示总初级生产力 （gross primary production，GPP） 和呼吸作用 （respiration，R） 过程之间的差异，它对碳收支平衡、海洋生态系统营养状态乃至气候变化等研究具有十分重要的指示意义。影响海洋 NEP 的因素有细菌、浮游生物、温度、太阳辐射、海冰融化、水团迁移、富营养有机质排放以及海水酸化等。目前计算 NEP 的方法可分为实验培养测定及数据模型计算两种。溶解氧培养法及同位素标记法等是经典的培养测定方法，但存在误差较大且重现性较差等问题。数据模型计算即借助养分质量平衡、响应面模型、O2/Ar 示踪等方法，通过将现场实测数据和生物地球化学模型结合，进行高时间分辨率的连续性观测，这也是目前测算 NEP 的主流应用手段。然而，相较于发达国家，我国在 NEP 的研究设备、技术、测定方法等方面仍存在一定差距。今后的研究重点将是建立 NEP 指标与表征海洋环境、气候变化之间的耦合关系以及 NEP 测定方法的改进，这将有助于深入理解和探索全球变化背景下海洋生态系统响应机制及变化趋势。     

栉孔扇贝精巢组织细胞的体外培养及特征分析

本研究建立了一个栉孔扇贝精巢组织细胞的原代培养体系,鉴定了原代细胞的功能基因表达特征。结果显示,原代培养体系中的精巢细胞以圆形细胞为主,直径为4~11μm,其在体外已存活9个月以上。使用栉孔扇贝vasa基因(生殖细胞标记基因)的地高辛RNA探针和原位杂交技术检测,约98%的原代细胞呈阳性。利用免疫组化技术鉴定了栉孔扇贝SCP3、KLF4、PIWI、C-MYC、SOX9和SOX17在这些细胞中的表达特征,发现这些靶蛋白在精巢原代细胞中的表达特征与栉孔扇贝精巢中的细胞定位基本一致。上述结果表明,本实验所获得的原代细胞以生殖细胞为主,它们保留了栉孔扇贝雄性生殖细胞原有的基因表达特征,可以用于贝类精子发生和性别相关的功能基因研究。     

适宜浓度的维生素E促进半滑舌鳎生长激素基因的表达

本实验旨在探究维生素E对半滑舌鳎垂体组织中生长激素基因表达的影响。作者在每千克等氮等能的基础饲料中分别添加0、400和1 600mg的DL-α-生育酚乙酸酯(维生素E)投喂半滑舌鳎(Cynoglossus semilaevis)成鱼(464±2.6) g,进行为期8周的养殖实验;另外,在L-15培养基中添加0、18和54μmol/L的维生素E,对半滑舌鳎成鱼(464±2.6) g的垂体细胞进行为期3 d的体外原代培养实验。分别取垂体组织和原代细胞,通过荧光实时定量PCR分析其gh mRNA的相对表达量。实验结果表明:垂体组织中gh mRNA的相对表达量随着饲料中维生素E含量的增加而呈现先升高后下降的变化趋势,在400 mg/kg组时显著高于其他各组(P0.05);随着细胞培养液中维生素E浓度的升高, gh mRNA的相对表达量显著增加(P0.05)。由此可见,适宜浓度的维生素E能够促进半滑舌鳎垂体组织中生长激素基因的表达。     

Nitrogen fixation driven by mesoscale eddies and the Kuroshio Current in the northern South China Sea and the East China Sea

N₂ fixation rates (NFR, in terms of N) in the northern South China Sea (nSCS) and the East China Sea (ECS) were measured using the acetylene reduction assay in summer and winter, 2009. NFR of the surface water ranged from 1.14 nmol/(L·d) to 10.40 nmol/(L·d) (average at (4.89±3.46) nmol/(L·d), n=11) in summer and 0.74 nmol/(L·d) to 29.45 nmol/(L·d) (average at (7.81±8.50) nmol/(L·d), n=15) in winter. Significant spatio-temporal heterogeneity emerged in our study: the anticyclonic eddies (AE) (P<0.01) and the Kuroshio Current (KC) (P<0.05) performed significantly higher NFR than that in the cyclonic eddies or no-eddy area (CEONE), indicating NFR was profoundly influenced by the physical process of the Kuroshio and mesoscale eddies. The depth-integrated N₂ fixation rates (INF, in terms of N) ranged from 52.4 μmol/(m2·d) to 905.2 μmol/(m2·d) (average at (428.9±305.5) μmol/(m2·d), n=15) in the winter. The contribution of surface NFR to primary production (PP) ranged from 1.7% to 18.5% in the summer, and the mean contribution of INF to new primary production (NPP) in the nSCS and ECS were estimated to be 11.0% and 36.7% in the winter. The contribution of INF to NPP (3.0%–93.9%) also decreased from oligotrophic sea toward the eutrophic waters affected by runoffs or the CEONE. Furthermore, we observed higher contributions compared to previous studies, revealing the vital roles of nitrogen fixation in sustaining the carbon pump of the nSCS and ECS.     

Assessment of carbon- and fluorescence-based primary productivity in Ariake Bay,southwestern Japan

A comparative account of primary productivity (PP), in the characteristically turbid and highly dynamic waters of Ariake Bay, measured by ¹³C uptake and fast repetition rate fluorometer (FRRF) was conducted to ensure compatibility between the two methods. Estimates from both methods depicted strong linearity for both short-term (r² > 0.90) and daily (r² = 0.42–0.93) measurements, except in the near-surface (∼0 m) layer. ¹³C-based short-term (1 h; in situ) PP estimates showed similar magnitudes and trend with the instantaneous PP measured by FRRF concurrently. Whereas, unlike short-term measurements, the daily PP estimates from both methods showed large difference, with FRRF-based time integrated daily PP resulting in 1.09–1.82 times higher than the carbon-based daily (24 h; simulated in situ) PP. This difference between daily PP estimates was mainly due to: (1) the temporal variation of water column chlorophyll a (Chl a) because of frequent moving of water mass, and (2) the dissimilarity in ambient light field conditions between the two methods. Results revealed that considering the above two environmental factors invariable over a daylength, fairly close approximation of daily PP, compared to ¹³C-based daily PP, could be obtained from FRRF. Hence, FRRF-based daily PP can be considered as more realistic in this highly dynamic water body like Ariake Bay where water column parameters are subjected to strong temporal variation. The relationship between Chl a-specific photosynthetic rate (P^B) and the corresponding photosynthetically active radiation (PAR) in the water column (PAR–P^B relationship) was found to be linear for FRRF and curvilinear for ¹³C-based measurements in the near-surface layer, for the same intensities of incident PAR, and this is thought to be the primary basis for the higher difference in PP estimates at the near-surface layer. Considering the minor variations in FRRF-based time series of PAR–P^B relationships, a combined and/or instantaneous PAR–P^B relationship in combination with incubation Chl a and light field condition was used to obtain fairly close estimates of daily water column integrated PP from FRRF.     

MJO prediction skill,predictability, and teleconnection impacts in the Beijing Climate Center Atmospheric General Circulation Model

This study evaluates performance of Madden–Julian oscillation (MJO) prediction in the Beijing Climate Center Atmospheric General Circulation Model (BCC_AGCM2.2). By using the real-time multivariate MJO (RMM) indices, it is shown that the MJO prediction skill of BCC_AGCM2.2 extends to about 16–17 days before the bivariate anomaly correlation coefficient drops to 0.5 and the root-mean-square error increases to the level of the climatological prediction. The prediction skill showed a seasonal dependence, with the highest skill occurring in boreal autumn, and a phase dependence with higher skill for predictions initiated from phases 2–4. The results of the MJO predictability analysis showed that the upper bounds of the prediction skill can be extended to 26 days by using a single-member estimate, and to 42 days by using the ensemble-mean estimate, which also exhibited an initial amplitude and phase dependence. The observed relationship between the MJO and the North Atlantic Oscillation was accurately reproduced by BCC_AGCM2.2 for most initial phases of the MJO, accompanied with the Rossby wave trains in the Northern Hemisphere extratropics driven by MJO convection forcing. Overall, BCC_AGCM2.2 displayed a significant ability to predict the MJO and its teleconnections without interacting with the ocean, which provided a useful tool for fully extracting the predictability source of subseasonal prediction.     

早白垩世泥炭地净初级生产力及其控制因素--来自二连盆地吉尔嘎郎图凹陷6号煤的证据

现代泥炭地中蕴藏着巨量的碳,泥炭地生产力的高低会影响全球碳循环及全球气候变化。前人对全新世以来泥炭地生产力做了大量研究,但对前第四纪的“深时”阶段的泥炭地生产力则极少涉及,其主要原因是缺少精确的定年方法。以二连盆地吉尔嘎郎图凹陷早白垩世6号煤为例,利用地球物理测井信号进行频谱分析并获得煤层中米兰科维奇旋回周期参数（123 ka（偏心率）:38.1 ka（斜率）:22.1 ka（岁差））,将米兰科维奇旋回作为“深时”地层时间的“度量”工具,计算出6号煤层碳的聚集速率为35.1~38.9 g C/（m2·a）,进一步推算出其所代表的泥炭地的碳聚集速率为46.2~51.2 g C/（m2·a）,净初级生产力（NPP）为231~256 g C/（m2·a）。将该计算结果与全新世同一纬度带泥炭地生产力水平比较,并结合前人研究成果综合分析影响因素,得出早白垩世泥炭地生产力水平主要受温度和大气中二氧化碳含量控制,而这两种因素又与气候相关联,则泥炭地生产力的研究可能对进一步了解古气候提供帮助。     

架子台沟低品位铅锌矿原生晕地球化学特征及深部矿产预测

对架子台沟一带1:50 000化探异常检查中,地表发现有铅锌矿化显示,经对激电测深异常深部钻探验证发现有低品位锌矿(化)体。通过系统采集0号勘探线上的2个钻孔中岩石、矿石样品,进行原生晕特征研究分析表明:矿体近矿元素为Ag、Pb、Zn、Cd,尾矿元素为W、Sn、Bi;轴向分带序列由上至下为:Pb-Au-Ag-Mo-Bi-Sn-Cu-Cd-W-Zn。对成矿元素与相关金属元素进行聚类分析、因子分析,并结合激电测深结果,认为在矿体的侧部、深部仍可能有盲矿体。     

西北太平洋海洋净初级生产力与柔鱼资源量变动关系的研究

海洋初级生产力在海洋生态中扮演重要角色,其变化影响了海洋渔业的潜在产量。本文根据2004-2013年中国鱿钓组提供的西北太平洋柔鱼(Ommastrephes bartramii)捕捞数据和海洋遥感净初级生产力数据,研究了柔鱼冬春生西部群体资源量变动与净初级生产力的关系。结果发现,柔鱼渔场范围内净初级生产力在经度方向上呈明显的季节性变化,冬春季低,夏秋季高。捕捞月份7-11月对应的适宜净初级生产力范围分别为500~700 mg/(m2·d)(以碳计),500~800 mg/(m2·d),500~1000 mg/(m2·d),500~800 mg/(m2·d)和300~500 mg/(m2·d),最适净初级生产力分别为700 mg/(m2·d),600 mg/(m2·d),700 mg/(m2·d),600 mg/(m2·d)和400 mg/(m2·d)。7-11各月最适净初级生产力平均纬度与捕捞努力量纬度重心呈显著正相关关系(P<0.05),说明了捕捞努力量位置在渔场中不是随机分布,可能受最适净初级生产力的纬度分布的影响。柔鱼年间资源丰度与各年3月份净初级生产力以及7-11月份平均净初级生产力大小显著正相关(P<0.05)。推测每年柔鱼资源量大小可能是由3月份产卵场海域和7-11月捕捞月份渔场净初级生产力水平交互作用的结果。研究表明,异常环境条件(厄尔尼诺和拉尼娜事件)对柔鱼产卵场和渔场的净初级生产力具有显著影响,但调控机制不同。