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51.
Sequence variation of the first internal transcribed spacer of ribosomal DNA ( ITS - 1 ) was examined and its application to the study of genetic variation was explored in four populations of farter' s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA (analysis of molecular variance) showed that the majority (96.26%) of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 ( = 0.042), indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly. 相似文献
52.
西沙礁序列的磁性地层学研究 总被引:3,自引:0,他引:3
利用西沙群岛西琛一井330m以上井段的149块岩心标本,用美国2-G型超导磁力仪进行了古地磁测定,获得了一个从更新世至中新世晚期包括布容,松山,高斯和吉尔伯特四个极性时及若干亚极性的磁性地层柱,为礁序列的划分对比,提供了新的依据。 相似文献
53.
Alessandro Buccolieri Giovanni Buccolieri Nicola Cardellicchio Angelo Dell'Atti Antonella Di Leo Antonella Maci 《Marine Chemistry》2006,99(1-4):227
Al, Cr, Cu, Fe, Hg, Mn, Ni, Pb, Ti and Zn concentrations have been determined in surface sediment samples collected in the Taranto Gulf (Ionian Sea, Southern Italy) in order to evaluate their levels and spatial distribution in this important area of the Mediterranean Sea. For various metals, the geoaccumulation index has been calculated as a criterion to assess if their concentrations represent contamination levels or can be considered as background levels. The results show that metals concentrations in sediments can be considered near the background levels found in the Mediterranean Sea.Analytical results have been elaborated by using a Geographical Information System (GIS) software to show metals accumulation areas. Using multivariate statistical analysis, we evaluate the possibility to distinguish the sampling stations, in relation to their geographical position. Results have showed metals distribution in the Taranto Gulf is principally influenced by industrial and urban wastes, located mostly in the northern coastal area of the Ionian Sea. Rivers in the Basilicata region and prevailing anticlockwise marine currents are further factors influencing metal accumulation in sediments. 相似文献
54.
边坡整体稳定的可靠性分析方法 总被引:8,自引:0,他引:8
边坡整体滑动稳定性的可靠性分析是建立在土体具有的抗力大于荷载效应的概率基础上进行设计和校验的。在条分法的基础上推导出了进行边坡稳定可靠性分析的统一极限状态方程 ,将土的容重γ、内摩擦角φ、粘聚力 c作为随机变量 ,当其为非正态分布时 ,进行当量正态化 ,考虑变量相关的情况 ,用简化相关法对随机变量进行统计分析 ,并以 Bishop圆弧滑动法为例 ,用 JC法求解边坡的可靠性指标及失效概率 ,给出计算安全系数和可靠性指标的算法程序。并就影响可靠性指标的因素如抗剪强度指标、变量分布形式、土性参数的变异性等进行了讨论。 相似文献
55.
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57.
辽东湾北部海域营养状况与趋势评价 总被引:3,自引:0,他引:3
根据 1999-2006 年 6-8 月的调查数据,分析辽东湾北部海域的营养水平及变化趋势,以了解辽东湾北部海域营养水平的分布特点及污染状况.由 E 值和 CN/CP 值的总体评价结果来看,辽河口、双台子河口和大凌河口海域的富营养化程度较高,属于磷中等限制潜在性富营养区,而锦州湾海域污染相对较轻,属于中度营养区;在时空分布上,辽东湾北部海域 1999 年与 2006 年污染较为严重,中间年份污染相对较轻.针对辽东湾的具体环境用 CN/CP 值的评价模式更能揭示出营养盐限制对富营养化的影响. 相似文献
58.
The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellae and N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337 bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher than that of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% tol 1.73%. In addition, UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other. Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether they belong to a single species or not are still undefined, and the more genes of them should be further investigated, in combination with the systematical and detailed morphological study. 相似文献
59.
地表土壤水分含量的时空分布信息是十分重要的,常常作为水文模型、气候模型、生态模型的输入参数,同时,也是干旱预报、农作物估产等工作的重要指标。被动微波遥感是监测土壤含水量最有效的手段之一。相比红外与可见光,它具有波长长,穿透能力强的优势。相比主动微波雷达,被动微波辐射计具有监测面积大、周期短,受粗糙度影响小,对土壤水分更为敏感,算法更为成熟的优势。目前,已研究出许多反演土壤水分的方法.本课题的主要内容是借助AMSR-E土壤水分影像数据、MODIS归一化植被指数(NDVI)影像数据和MODIS分类影像数据,利用ENVI软件进行遥感图像数据处理,运用统计分析方法建立NDVI与土壤水分的经验模型,研究中国西部地区稀疏植被覆盖区土壤水分的反演。 相似文献
60.
Multiple source rock assemblages were deposited in the sedimentary provinces in South China in geologic history,and some of them were destructed by and some survived against multiple tectonic movements.Therefore,multiple sources,mixed sources,and uneven distribution of sources occurred in the marine sedimentary basins in South China during the late stage of hydrocarbon pooling.Epidiagenesis of the marine carbonate reservoirs and its modification to reservoir poroperm characteristics determined the formation and the scale of natural gas pools.The exploration practices show that the large to medium gas fields mainly occur in areas with high-quality reservoirs.Detailed study of the paleo-oil accumulations and typical oil and gas reservoirs reveals that the basins experienced multiphase superimposition and modification,leading to the distribution of the Paleozoic paleo-oil accumulations and bitumen in the peripheral areas.The phenomenon that oil and gas production concentrates in the Sichuan basin indicates that the overall sealing conditions of a basin determine the oil/gas potentials and the scale of oil and gas production.This is a critical factor controlling the accumulation and distribution of gas in the marine sequences in South China.The early oil and gas pools in the Yangtze platform left billions of bitumen in the peripheral areas due to the destruction of seals.Since the Himalayan,"late-generation and late-accumulation" gas pools represented by the gas pools in the Sichuan (四川) basin were formed in the marine sedimentary sequences in South China as a result of the change of the sealing conditions.Current gas discoveries appear to be "paleo-generation and paleo-accumulation" gas pools but actually are "late-generation and late-accumulation" gas pools.These patterns of hydrocarbon pooling clearly depict themselves in western Sichuan basin and Weiyuan (威远)gas field.It is revealed that the gas pools in the Sichuan basin were mainly formed as a result of hydrocarbon phase change (thermal cracking of oil to gas),miscible migration,and dynamic equilibration since the Himalayan.A large number of gas pools were formed in the Himalayan and the gas pools in the marine sequences are characterized by late pooling; this kind of gas fields/pools are controlled by:(1) effectiveness of modification and superimposition of the marine basins,(2) effectiveness of the source rocks,(3) effectiveness of the overall preservation conditions,and (4) effectiveness of plays. 相似文献