Particulate peptidoglycan in seawater determined by the silkworm larvae plasma (SLP) assay

Peptidoglycan (PG) is a biopolymer found exclusively in the cell wall of bacteria. Recent chemical analysis of particulate organic matter suggests that a major amount of the muramic acid, an amino sugar present only in PG, could not be accounted for in terms of bacterial cells (Benner and Kaiser, 2003); however, data on particulate PG is quite sparse. In the present study, conducted in 1996, the PG was examined at 5 sampling sites in the northwestern Pacific Ocean, and in natural seawater cultures. Particulate PG, which was concentrated using a 96-well filtration plate equipped with Durapore filters (pore size, 0.22 μm), was measured by the silkworm larvae plasma (SLP) assay. The PG concentration generally decreased with depth and correlated significantly with bacterial abundance throughout the entire water column. However, the ratio of particulate PG to bacterial abundance varied with depth. The average ratio was 0.61 ± 0.53 (average ± SD, n = 40) between 50 and 2000 m, which agreed with the bacterial cellular PG content from 0.63 to 1.1 fg cell⁻¹ obtained in seawater cultures. On the other hand, the ratios of PG to bacteria from the surface to 50 m (3.7 ± 2.6, n = 29) and below 2,000 m (2.1 ± 1.7, n = 7) were significantly higher than that between 50 and 2,000 m. These results may suggest that, in the surface and deep layers, a significant fraction of particulate PG was present in bacterial detritus, whereas this fraction was reduced in the middle layer.     

现场海域水平扩散系数的探讨 Ⅱ．黄河三角洲埕北海域的计算

根据Ｔａｙｌｏｒ的湍流扩散理论和Ｈａｙ、Ｐａｓｑｕｉｌｌ提出的拉格朗日流和欧拉流的相关函数的相似性，用黄河三角洲埕北海域的实测资料，按四种不同方案计算出该海域的水平扩散系数和谱密度分布。指出：该海区潮流扩散系数主要受潮流的影响，其分布特征与潮流的分布基本一致，所求的水平扩散系数主要是由周期性的潮运动给予潮流扰动所致。方案４是通过富氏变换滤波消去了周期性潮流扰动，本方案计算的水平扩散系数是由周期为６ｈ以下的小尺度涡提供能量。     

An inexpensive instrument for measuring benthic current velocity and direction at sea

An instrument which measures the velocity and direction of benthic water currents at depths of up to 80 m is described. Characteristics of this meter include: (i) readings are not affected by water flow during sinking or retrieval; (ii) it is portable; (iii) it operates unattended; (iv) it is inexpensive; and (v) it requires only simple maintenance. A timing circuit which operates two time periods in succession is used to activate and de-activate a revolution counter and a fluidfilled compass which are mounted on a special frame and vane system. In the field, the instrument accurately measured average current velocities of up to 6 m s⁻¹. Data are presented which illustrate the usefulness of this instrument in describing the velocity and direction of benthic currents along the east coast of Australia and demonstrate a positive correlation between benthic current velocity and catch rates of the commercially-exploited spanner crab Ranina ranina.     

连云港近岸海域物理自净能力研究及水质预测——Ⅲ.西大堤建成后对物质输运的影响

在连云港近岸海域计算潮流场基础上建立拉格朗日余流模型,并对连云港市两大堤建成前后的拉格朗日余流变化进行了分析,且选择有代表性的排污口进行了数值跟踪。     

钝顶螺旋藻部分原生质体及单细胞的制备与培养

于1992年1月-1994年4月，进行超声波处理方法制备钝顶螺旋藻部分原生质体以作为基因工程受体，以及制备单细胞用于固体平板克隆化培养的研究。研究结果表明：超声波以20kHz频率、15W功率作用30s，可使藻丝体断裂成15．0±1．6个细胞长度；延长作用时间，至2－6个细胞长度时，细胞壁结构遭到破坏，形成部分原生质体；继续作用，可形成少量原生质体和大量单细胞。断裂藻丝体、部分原生质体、单细胞以及原生质体均可涂布于固体培养基上再生或生长。以一定密度涂布单细胞与原生质体，能够形成彼此分开的单个克隆，可用于筛选及遗传分析。本文提供了一种节省溶菌酶的制备螺旋藻透性体的方法，超声波作用利于外源基因的导入，而涂布培养利于进一步的筛选和形成克隆。     

Frequency-selective attenuation of sound propagation and reverberation in shallow water

Ｆｒｅｑｕｅｎｃｙ－ｓｅｌｅｃｔｉｖｅ　ａｔｔｅｎｕａｔｉｏｎ　ｏｆ　ｓｏｕｎｄ　ｐｒｏｐａｇａｉｏｎ　ａｎｄ　ｒｅｖｅｒｂｅｒａｔｉｏｎ　ｉｎ　ｓｈａｌｌｏｗ　ｗａｔｅｒTXＦｒｅｑｕｅｎｃｙ－ｓｅｌｅｃｔｉｖｅａｔｔｅｎｕａｔｉｏｎｏｆｓｏｕｎｄｐ...     

水槽造流系统水力计算与泵机选择

提供一种完整的用于试验水槽造流系统的水力计算和泵机选择方法 ,可用于其他类似工程设计。     

胶州湾三维变动边界的潮流数值模拟

基于Blumberg等（1986）的河口、陆架和海洋模式，引入变边界处理技术，建立了胶州湾三维变动边界的潮流模型，模型以σ-正交曲线坐标下三维非线性潮波方程为基本方程，引入2.5阶瑞封闭方程组，采用分裂算子法数值求解方程组，利用湍封闭方程求解垂直紊动粘滞系数，采用干湿网格方法模拟潮流漫滩过程，三维变动边界潮流模型计算结果与实测值吻合良好。     

完全非线性波浪破碎模型沿岸流数值模拟

探讨一种基于完全非线性Boussinesq方程的波浪破碎模型在沿岸流计算中的应用问题。针对控制方程中的完全非线性项对沿岸流成长过程的影响进行了深入讨论。数学模型计算结果表明,完全非线性项有使平均流局部化的作用;通过数模实验还发现,垂向高阶涡度项可以有效抑制破波区外回流;运用Visser的实验室沿岸流实测资料从沿岸流速度、波高和平均水位几方面对所提模型进行了验证,并给出了紊动参数的计算结果。