鱼类生长激素的异源表达、应用及安全性评价

鱼类生长激素对鱼的生长发育起着重要的调节作用，能促进鱼体快速生长，提高饵料转化率，在水产养殖业中具有重大应用价值。转生长激素基因鱼具有快速生长效应，可以缩短养殖周期，降低养殖成本和风险。鱼类生长激素基因异源表达体系的建立和发展，使获得大量廉价的鱼类生长激素产品成为可能，为鱼类养殖业新型饵料添加剂的研制开辟了新的途径。本文综述了转生长激素基因鱼和鱼类生长激素基因的异源表达两方面的研究及其安全性评价。     

A Reconstruction of Observed Profiles in the Sea East of Japan Using Vertical Coupled Temperature-Salinity EOF Modes

It is important to estimate hard-to-observe parameters in the ocean interior from easy-to-observe parameters. This study therefore demostrates a reconstruction of observed temperature and salinity profiles of the sea east of Japan (30°≈40°N, 140°≈150°E). The reconstruction was done by estimating suboptimal state from several values of the observed profiles and/or sea surface dynamic height (SDH) calculated from the profiles. The estimation used a variational method with vertical coupled temperature-salinity empirical orthogonal function (EOF) modes. Profiles of temperature and salinity in the subtropical region are effectively reconstructed from in situ temperature profile data, or sea surface temperature (SST) and SDH. For example, the analyzed temperature field from SST and SDH has an accuracy to within 1°C in the subtropical region. Salinity in the sea north of Kuroshio, however, is difficult to estimate because of its complex variability which is less correlated with temperature than in the subtropical region. Sea surface salinity is useful to estimate the subsurface structure. We also show the possibility that the estimation is improved by considering nonlinearity in the equation calculating SDH from temperature and salinity analysis values in order to examine the misfit between analysis and observation. Analysis using TOPEX/POSEIDON altimetry data instead of SDH was also performed. This revised version was published online in July 2006 with corrections to the Cover Date.     

Seakeeping assessment of fishing vessels in conceptual design stage

The main idea of this paper is to identify functional relations between seakeeping characteristics and hull form parameters of Mediterranean fishing vessels. Multiple regression analysis is used for quantitative assessment through a computer software that is based on the SQL Server Database. The seakeeping attributes under investigation are the transfer functions of heave and pitch motions and of absolute vertical acceleration at stern, while the ship parameters influencing motion dynamics have been classified into two groups: displacement (Δ) and main dimensions (L, B, T), coefficients that define the details of the hull form (C_WP, C_VP, LCB, LCF, etc.).Four multiple regression models having different parameter combinations are here investigated and discussed, giving way to the so-called ‘Simple Model’, ‘Intermediate Model’, ‘Enhanced 1 Model’ and ‘Enhanced 2 Model’. The obtained results are more than satisfactory for seakeeping predictions during the conceptual design stage.     

Preconditioned Optimizing Utility for Large-dimensional analyses (POpULar)

I present the derivation of the Preconditioned Optimizing Utility for Large-dimensional analyses (POpULar), which is developed for adopting a non-diagonal background error covariance matrix in nonlinear variational analyses (i.e., analyses employing a non-quadratic cost function). POpULar is based on the idea of a linear preconditioned conjugate gradient method widely adopted in ocean data assimilation systems. POpULar uses the background error covariance matrix as a preconditioner without any decomposition of the matrix. This preconditioning accelerates the convergence. Moreover, the inverse of the matrix is not required. POpULar therefore allows us easily to handle the correlations among deviations of control variables (i.e., the variables which will be analyzed) from their background in nonlinear problems. In order to demonstrate the usefulness of POpULar, we illustrate two effects which are often neglected in studies of ocean data assimilation before. One is the effect of correlations among the deviations of control variables in an adjoint analysis. The other is the nonlinear effect of sea surface dynamic height calculation required when sea surface height observation is employed in a three-dimensional ocean analysis. As the results, these effects are not so small to neglect.     

Risk-based decision-making for drilling waste discharges using a fuzzy synthetic evaluation technique

Offshore petroleum drilling wastes contain toxic substances that are potentially harmful to the marine ecosystem. Despite environmentally benign characteristics, wastes associated with synthetic-based fluids still contain a certain amount of pollutants due to contamination with formation oil and the presence of trace heavy metals in barite, which may pose risk when discharged into the marine environment. A framework is presented here for a decision support system for the selection of the best drilling waste discharge option. Uncertainties in the quantification of risk, cost and technical feasibility are expressed by fuzzy numbers. An analytical hierarchy process with a technique called fuzzy synthetic evaluation is employed to determine the best management alternative (discharge scenario).     

关于海水网箱养殖系统风险评估的基础研究

随着海水网箱养殖业的迅速发展,制约海水网箱养殖发展的种种因素也逐步显露出来(风暴潮等恶劣气候、环境污染、人为的破坏等等),海水网箱养殖在预期获得丰厚利润的同时也面临遭受巨大损失的风险。本文对海水网箱养殖系统风险评估的可操作性进行了基础研究,提出了一套适合海水网箱养殖系统的风险评估方法,并就数据的收集和专家评审表做了一些研究,以此客观的反映系统的安全性和可靠性,使决策者实现对养殖系统生命周期的最佳控制。     

山东半岛东部,北部地区冬季冷流低云的探讨

本文主要对山东半岛东部、北部及渤海海峡地区的地方性天气－冷流低云，作了初步的分析。着重分析产生这种低云的环流特点及殊地理环境，分析归纳出这种低云的预测方法及判据。     

Theoretical aspects of cap-rock and fault seals for single- and two-phase hydrocarbon columns

Cap-rock seals can be divided genetically into those that fail by capillary leakage (membrane seals) and those whose capillary entry pressures are so high that seal failure preferentially occurs by fracturing and/or wedging open of faults (hydraulic seals). A given membrane seal can trap a larger oil column than gas column at shallow depths, but below a critical depth (interval), gas is more easily sealed than oil. This critical depth increases with lower API gravity, lower oil GOR and overpressured conditions (for the gas phase). These observations arise from a series of modelling studies of membrane sealing and can be conveniently represented using pressure/ depth (P/D) profiles through sealed hydrocarbon columns. P/D diagrams have been applied to the more complex situation of the membrane sealing of a gas cap underlain by an oil rim; at seal capacity, such a two-phase column will be always greater than if only oil or gas occurs below the seal.These conclusions contrast with those for hydraulic seals where the seal capacity to oil always exceeds that for gas. Moreover, a trapped two-phase column, at hydraulic seal capacity will be less than the maximum-allowed oil-only column, but more than the maximum gas-only column. Unlike membrane seals, hydraulic seal capacity should be directly related to cap-rock thickness, in addition to the magnitude of the minimum effective stress in the sealing layer and the degree of overpressure development in the sequence as a whole.Fault-related seals are effectively analogous to membrane cap-rocks which have been tilted to the angle of the fault plane. Consequently, all of the above conclusions derived for membrane cap-rocks apply to both sealing faults sensu stricto (fault plane itself seals) and juxtaposition faults (hydrocarbon trapped laterally against a juxtaposed sealing unit). The maximum-allowed two-phase column trapped by a sealing fault is greater than for equivalent oil-only and gas-only columns, but less than that predicted for a horizontal membrane cap-rock under similar conditions. Where a two-phase column is present on both sides of a sealing fault (which is at two-phase seal capacity), a deeper oil/water contact (OWC) in one fault block is associated with a deeper gas/oil contact (GOC) compared with the adjacent fault block. If the fault seal is discontinuous in the gas leg, however, the deeper OWC is accompanied by a shallower GOC, whereas a break in the fault seal in the oil leg results in a common OWC in both fault blocks, even though separate GOC's exist. Schematic P/D profiles are provided for each of the above situations from which a series of fundamental equations governing single- and two-phase cap-rock and fault seal capacities can be derived. These relationships may have significant implications for exploration prospect appraisal exercises where more meaningful estimates of differential seal capacities can be made.The membrane sealing theory developed herein assumes that all reservoirs and seals are water-wet and no hydrodynamic flow exists. The conclusions on membrane seal capacity place constraints on the migration efficiency of gas along low-permeabiligy paths at depth where fracturing, wedging open of faults and/or diffusion process may be more important. Contrary to previous assertions, it is speculated that leakage of hydrocarbons through membrane seals occurs in distinct pulses such that the seal is at or near the theoretically calculated seal capacity, once this has been initially attained.Finally, the developed seal theory and P/D profile concepts are applied to a series of development geological problems including the effects of differential depletion, and degree of aquifer support, on sealing fault leakage, and the evaluation of barriers to vertical cross-flow using RFT profiles through depleted reservoirs. It is shown that imbibition processes and dynamic effects related to active cross-flow across such barriers often preclude quantitative analysis and solution of these problems for which simulation studies are usually required.     

运用德尔菲调查—灰色统计法确立水库鱼产力综合评价中的指标权重体系

本文运用德尔菲方法对水库鱼产力综合评价中指标权重的合理分配问题作了专家调查,并采用灰色统计法对调查结果进行归纳处理,从而确立了一个水库鱼产力影响因素诸层次各方面的评价指标权重体系,可供今后的评价工作参考使用。