三株赤潮硅藻5.8SrDNA及转录间隔区(ITS)的克隆及序列分析

对引发赤潮的3株硅藻——1株尖刺拟菱形藻(Pseudo-nitzshia pungens)和2株中肋骨条藻(Skeletonema costatum)的5.8SrDNA和ITS(internal transcribed spacers)序列进行了PCR扩增、克隆和序列测定,并分析了硅藻门10株赤潮藻(7株从GenBank获得)的系统进化关系.研究结果表明,尖刺拟菱形藻的ITS和5.8SrDNA的长度为693bp,SK-1(分离自东海赤潮暴发区)测序得到715bp,除ITS和5.8SrDNA外,还包含部分18SrDNA和28SrDNA;SK-2(分离自青岛养殖场)的ITS和5.8SrDNA的长度为331bp,尖刺拟菱形藻与从GenBank中获得的2株尖刺拟菱形藻相似程度最高,为100%,与该属的多列拟菱形藻相似程度稍低,为82.9%.SK-2的ITS序列与SK-1的相似程度很低,只有51%,但与拟中肋骨条藻的ITS序列相似程度高,为95.5%.SK-1的ITS序列与拟中肋骨条藻的相似程度也低,为56.7%.系统进化树反映的结果与相似性反映的结果一致.研究的该株尖刺拟菱形藻从根据ITS序列研究的结果与形态鉴定的结果看是一致的;SK-2可能属于拟中肋骨条藻;SK-1比较特殊,有待于用其他的方法进一步研究确定其分类地位.     

华南沿海地区全新世海平面变化特征

１海平面变化特征１．１全新世中期海侵根据地层中所含硅藻属种雷州半岛海康南田乡一１５ｍ钻孔岩芯，含有丰富的硅藻，主要属种是①：具槽直链藻（Ｍｅｌｏｓｉｒａ　ｓｕｌｃａｔａ（Ｅｈｒ）Ｋｕｔｚ）、范氏回箱藻（Ｐｙｘｉｃｌｉｃｕｌａ　ｗｅｙｐｒｅｃｈｔｉｉＧｒｕｎ）、条纹小环藻（Ｃｙｃｌｏｔｅｌｌａｓｔｒｉａｔａ（Ｋｕｔｚ）Ｇｒｕｎ）、流水双菱藻（ＳｕｒｉｒｅｌｌａｆｌｕｍｉｎｅｎｓｉｓＧｒｕｎ）。上述硅藻主要是海相生活属种，今日南田乡已距离海岸线５ｋｍ。徐闻海安西侧一１８．５ｍ钻孔岩芯中的硅藻除上述属…     

Genetic relationship between Neobenedenia girellae and N. melleni inferred from 28S rRNA sequences

The fragments of 350 bp in 28S rRNA from the closely related monogenea of trematoda, Neobenedenia girellae and N. melleni are obtained by polymerase chain reaction (PCR) amplified using a couple of special primers and then sequenced. The results show that the comparison of 28S rRNA sequences, with only a base varying in 337 bp accounting for 0.3% genetic difference, from the relative species N. girellae and N. melleni parasitized on the different fishes in different farms displays that they possess a very high genetic similarity of 99.7%, higher than that of 99.41% for the single species N. melleni sampled in different areas, and the intraspecific divergence of N.melleni is 0.59%. Meanwhile, the interspecific differences between the two Neobenedenia and three Benedenia (i.e., B. lutjani, B. rohdei and B. seriolae) range from 2.08% tol 1.73%. In addition, UPGMA and MP molecular phylogenetic trees are constructed and proved to be consistent with each other. Though the morphological characteristics and the results of genetic diversity for the two Neobenedenia show a high similarity, whether they belong to a single species or not are still undefined, and the more genes of them should be further investigated, in combination with the systematical and detailed morphological study.     

Main Controlling Factors on Hydrocarbon Accumulation and Distribution in Marine Sedimentary Sequences in South China

Multiple source rock assemblages were deposited in the sedimentary provinces in South China in geologic history,and some of them were destructed by and some survived against multiple tectonic movements.Therefore,multiple sources,mixed sources,and uneven distribution of sources occurred in the marine sedimentary basins in South China during the late stage of hydrocarbon pooling.Epidiagenesis of the marine carbonate reservoirs and its modification to reservoir poroperm characteristics determined the formation and the scale of natural gas pools.The exploration practices show that the large to medium gas fields mainly occur in areas with high-quality reservoirs.Detailed study of the paleo-oil accumulations and typical oil and gas reservoirs reveals that the basins experienced multiphase superimposition and modification,leading to the distribution of the Paleozoic paleo-oil accumulations and bitumen in the peripheral areas.The phenomenon that oil and gas production concentrates in the Sichuan basin indicates that the overall sealing conditions of a basin determine the oil/gas potentials and the scale of oil and gas production.This is a critical factor controlling the accumulation and distribution of gas in the marine sequences in South China.The early oil and gas pools in the Yangtze platform left billions of bitumen in the peripheral areas due to the destruction of seals.Since the Himalayan,"late-generation and late-accumulation" gas pools represented by the gas pools in the Sichuan (四川) basin were formed in the marine sedimentary sequences in South China as a result of the change of the sealing conditions.Current gas discoveries appear to be "paleo-generation and paleo-accumulation" gas pools but actually are "late-generation and late-accumulation" gas pools.These patterns of hydrocarbon pooling clearly depict themselves in western Sichuan basin and Weiyuan (威远)gas field.It is revealed that the gas pools in the Sichuan basin were mainly formed as a result of hydrocarbon phase change (thermal cracking of oil to gas),miscible migration,and dynamic equilibration since the Himalayan.A large number of gas pools were formed in the Himalayan and the gas pools in the marine sequences are characterized by late pooling; this kind of gas fields/pools are controlled by:(1) effectiveness of modification and superimposition of the marine basins,(2) effectiveness of the source rocks,(3) effectiveness of the overall preservation conditions,and (4) effectiveness of plays.     

BP神经网络模型在澜沧江流域径流量模拟中的应用

河川径流等水文时间序列属于复杂的非线性系统,使用回归分析等传统的分析方法,难以获取和描述其内在关联和变化规律。利用现有的相关站点的径流量历史数据和输沙量、降水量数据,在进行规格化处理和主成分分析的基础上,利用三层BP人工神经网络模型,对澜沧江流域上游昌都站径流量与各关联因子之间复杂的非线性映射关系进行模拟,采用拟牛顿算法对模型进行训练,模拟结果达到期望精度要求,并利用1982年~1985年实测数据进行模型验证。结果证明利用BP神经网络模型对澜沧江流域站点的月径流量序列进行模拟、预测和数据补缺处理具有可行性。     

东亚夏季风石笋记录中缺失格陵兰间冰段25?

东亚季风是全球气候系统的重要组成部分[1~6]。了解北大西洋地区温度变化和东亚夏季风之间的关系对了解东亚季风的控制机制具有重要意义。葫芦洞和董哥洞石笋δ18O记录显示距今75~10ka北大西洋地区千年尺度温度变化对东亚夏季风变化具有重要影响:较强的东亚夏季风与北大西洋地区较高气温对应,反之亦然[7,8]。但是,在此之前东亚夏季风变化与北大西洋地区温度变化之间是否具有类似的联系?到目前为止对这一问题尚没有明确的探讨。根据最近分别在意大利北部[9]和中国中部获得的石笋δ18O记录,笔者发现,北大西洋地区冰芯记录的间冰段(Greenland…     

国内部分IGS站点汶川地震前后的时变特征

在介绍各种GPS静态方法和动态定位方法的基础上,对我国武汉及昆明IGS站点在汶川地震时的时变特征进行了分析,通过数据处理可以发现,IGS跟踪站点在地震前后的动态结果无论是采用双差结果还是精密单点定位结果,都显示其有明显的变化,而采用动态定位更能显示这种变化。同时,IGS跟踪站点长期变化还无法反映地震的前兆影响。     

矿井涌水量混沌时间序列分析与预测

矿井地下水系统是个非线性系统,由于地下水系统的复杂性,很难完全确定影响矿井涌水量的因素,因此传统的矿井涌水预测表现出较大的局限性。而混沌时间序列分析能够利用单变量处理方法,分析矿井地下水系统中,由于多因素耦合作用所产生的矿井涌水量时间序列。通过对某矿区1985年9月至2005年2月矿井涌水量时间序列资料的分析,显示矿井涌水量时间序列具有混沌特征;通过对比预测值和实测值,表明预测精度高。因此,混沌时间序列分析方法用于矿井涌水量预测是可行的。     

水文时间序列周期识别的新思路与两种新方法

针对水文序列周期识别的困难,提出首先对原序列处理,再识别周期的新思路,同时提出两种新方法:一种是模拟延长序列法,即通过建模延长原序列,再应用最大熵谱分析法(MESA)对延长序列识别周期;另一种方法是构建主频序列法,应用小波重构法重构原序列主频部分,然后应用MESA对重构序列进行周期识别。结合实例,运用多种方法对同一序列进行周期识别。分析结果表明:由于受序列长度偏短、偏态性、复杂随机成分等因素的影响,传统单一处理方法(周期图法、FFT、MESA、小波分析)周期识别效果并不理想,而使用两种新方法可以有效地减小或消除上述因素的影响,周期识别效果有明显改善。