船上气温、湿度和风速观测的代表性

当前我国有许多海洋气象调查船、商船和遥测气象浮标站的甲板上安装有气温、湿度和风速测量仪器，其中不少仪器本身测量精度是符合要求的，可是在船上使用能否获取符合要求的气象资料，其代表性如何，即是否能准确代表和反映观测船所在地点一定     

环境演变研究时间序列的建立方法评述

对环境演变时间序列建立的几种主要方法的纪年原理、应用状况及应用的优点、范围、局限性进行了阐述和比较，包括长寿命宇宙成因核素在内的同位素纪年方法主要判别第四纪以来大时间尺主的沉积物的地质年龄，且能很好地应用于全球变化的研究，但百余年来沉积物的年龄及侵蚀历史变化的研究只能通过^210Pbex、^137Cs等半衰期相对较短的核素来进行；指出^210Pbex可望在现代流域侵蚀环境演变以及其效应评价的研究中获得新的突破。     

东海原甲藻cDNA文库构建及尝试性EST分析

东海原甲藻(Prorocentrumdonghaiense)是中国沿海频繁发生的大规模赤潮的原因种之一,大规模分离鉴定东海原甲藻功能基因是理解东海原甲藻赤潮形成过程和机理的重要基础。取对数生长期藻体,经微量总RNA抽提、cDNA合成、cDNA扩增和克隆等步骤,构建了东海原甲藻cDNA文库并进行了尝试性表达序列标签分析。从含有约5000个转化子的文库中随机取150个测序,获得126条EST序列。经网上BlastN及BlastX分析,共发现11个是已知功能的基因的标签。这些基因与东海原甲藻生长发育、物质转换和能量代谢相关。     

青岛近百年气温变化的统计分析

本文依据青岛１８９８－１９９４年９７年的气温资料，经统计分析，得出了青岛气温的阶段和周期及增温速率，探讨了影响变化几个因子的定性关系，并预测未来气温的长期变化趋势。     

Hydrocarbon Potential of Pre-cenozoic Strata in the North Yellow Sea Basin

The North Yellow Sea Basin ( NYSB ), which was developed on the basement of North China (Huabei) continental block, is a typical continental Mesozoic Cenozoic sedimentary basin in the sea area. Its Mesozoic basin is a residual basin, below which there is probably a larger Paleozoic sedimentary basin. The North Yellow Sea Basin comprises four sags and three uplifts. Of them, the eastern sag is a Mesozoic Cenozoic sedimentary sag in NYSB and has the biggest sediment thickness; the current Korean drilling wells are concentrated in the eastern sag. This sag is comparatively rich in oil and gas resources and thus has a relatively good petroleum prospect in the sea. The central sag has also accommodated thick Mesozoic-Cenozoic sediments. The latest research results show that there are three series of hydrocarbon source rocks in the North Yellow Sea Basin, namely, black shales of the Paleogene, Jurassic and Cretaceous. The principal hydrocarbon source rocks in NYSB are the Mesozoic black shale. According to the drilling data of Korea, the black shales of the Paleogene, Jurassic and Cretaceous have all come up to the standards of good and mature source rocks. The NYSB owns an intact system of oil generation, reservoir and capping rocks that can help hydrocarbon to form in the basin and thus it has the great potential of oil and gas. The vertical distribution of the hydrocarbon resources is mainly considered to be in the Cretaceous and then in the Jurassic.     

南极冰川变化莫测,各国专家说法不一

南极冰川变化莫测，各国专家说法不一英美科学家：气候变暖引起南极冰架脱落据美国《波士顿环球报》报道，英国科学家在《自然》杂志上发表报告说，全球气候变暖将导致南极冰架融化脱落，并且脱落的浮冰很可能会引起海平面上升．英国研究人员说，南极半岛气温的持续上升使...     

中国花鲈细胞色素b基因片段的序列研究(英文)

参考鳗鲡等鱼类线粒体 DNA序列进行了中国花鲈线粒体 DNA细胞色素 b基因片断的引物设计、PCR扩增及其序列测定。得到中国花鲈的碱基序列为 4 10 bp,其 A、T、G、C含量分别为 10 1bp(2 4 .6 3% )、112 bp(2 7.32 % )、72 bp(17.56 % )、12 5bp(30 .4 9% ) ,与鳗鲡等其他鱼类相同基因片断序列碱基含量相似。     

Application of the first internal transcribed spacer(ITS-1) of ribosomal DNA as a molecular marker to population analysis in farrer''s scallop Chlamys farreri

Sequence variation of the first internal transcribed spacer of ribosomal DNA （ ITS - 1 ） was examined and its application to the study of genetic variation was explored in four populations of farter＇ s scallop Chlamys farreri. ITS - 1 fragments, with a length of about 300 bp,of 78 individuals collected from Dalian, Qingdao, Yantai in China and Korea respectively were amplified via PCR, cloned and sequenced. Intra-genomic variation was examined by sequencing several clones of single individuals. Alignment and polymorphism analysis detected 44 haplotypes and 50 polymorphic sites which consist of 30 substitutions and 20 indels, indicating a high level of polymorphisms. Sequence analysis also showed a very low level of intra-individual variation. All these features validated the feasibility of application of ITS - 1 fragment to population analysis. Polymorphism analysis showed that the Korea sample has the richest genetic variation, followed by Yantai and Qingdao samples. AMOVA （analysis of molecular variance） showed that the majority （96.26%） of genetic variation was distributed within populations and 3.74% resulted from among populations, but with P 〈 0.05 （ = 0.042）, indicating that the populations in this study have significant divergence. This output was basically concordant with the result arising from RAPD data and different from that from mitochondrial 16S rDNA sequence data. Discussion on this inconsistency was made accordingly.     

西沙礁序列的磁性地层学研究

利用西沙群岛西琛一井３３０ｍ以上井段的１４９块岩心标本，用美国２－Ｇ型超导磁力仪进行了古地磁测定，获得了一个从更新世至中新世晚期包括布容，松山，高斯和吉尔伯特四个极性时及若干亚极性的磁性地层柱，为礁序列的划分对比，提供了新的依据。