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Carole Gaüzère Marina Moletta‐Denat Faisl Bousta Stéphane Moularat Geneviève Orial Sébastien Ritoux Jean‐Jacques Godon Enric Robine 《洁净——土壤、空气、水》2013,41(3):226-234
Biological aerosols from air constitute a significant source of exposure to microorganisms in public places. Airborne microorganisms are involved in the development of certain respiratory symptoms, allergies, or infections among users and occupants. Various sampling instruments have commonly been used in aerobiology to collect bacteria and fungi suspended in the air. The objective of this study was to develop a reliable procedure for sampling in indoor public environments presenting different levels of occupancy, airborne bacteria and fungi to be subjected to molecular analysis (bacteria and fungi quantitative PCR, capillary electrophoresis single strand conformation polymorphism fingerprinting). Four different sampling devices were tested in situ in an office building (open‐plan type) and the sampling strategy chosen was tested in two museum contexts. In accordance with the drawbacks involved to our study (quantitative and qualitative aspects, cost, and overcrowding), cyclone device appeared to be most suitable. The results underline the effectiveness of this high‐volume aerosol sampling device for both qualitative and quantitative molecular analysis. Four in situ sampling collections were carried out in 1 day in the Louvre Museum to study quantitative and qualitative variations of airborne bacterial and fungal diversity. The quantitative results revealed a similar order of magnitude for the numbers of both bacteria and fungi. In the Louvre Museum, the samples yielded between 3.7 × 104 and 4.1 × 104 genome equivalent (GE) bacteria/m3 air and between 5.0 × 104 and 5.9 × 104 GE fungi/m3 air and in the Decorative Arts Museum between, 2.1 × 104 and 2.5 × 104 GE bacteria/m3 air and between 1.4 × 104 and 1.7 × 104 GE fungi/m3 air. The results also indicate that the dominant bacterial community displayed a stable structure over a short period of time whereas dominant eukaryotic airborne community appeared more variable. 相似文献
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“野外地质掌中宝”系统的及其应用 总被引:2,自引:0,他引:2
“野外地质掌中宝”系统是以Windows CE为软件平台,以掌上电脑为硬件载体, 适用于野外地质勘探和国土资源调查的便携式工作平台。它包括前端工具软件包、后台伺服处理子外地质勘探和国土资源调查技术的一次重要实践,它的研制成功将为野外地质工作才提供面向21的高效、实用、方便的现代化工作平台。 相似文献
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计算机远程控制是地震台站的未来发展方向,利用Windows XP可以实现单位业务的远程处理,切实提高工作效率。 相似文献
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基于PDA的嵌入式移动GIS系统应用研究与开发 总被引:12,自引:1,他引:11
PDA作为嵌入式移动GIS的载体,在城市旅游、个人定位和车辆导航等方面存在着巨大的潜力。笔者结合自行研制的煤、水、电抢修PDA嵌入式移动GIS电子地图导航系统,从系统开发平台与开发工具的选择、系统架构与功能、实现的关键技术等方面进行了详细的介绍,重点研究了无线网络模式数据传输的交互技术。 相似文献
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首先介绍了系统开发环境,系统采用EVC基于Windows CE进行开发;接着介绍了ADO对象与ADOCE对象的异同点、Windows CE平台下的数据存储格式、数据库开发环境的配置;最后,文章讨论了车辆导航系统中如何利用ADOCE访问Access数据库实现地物查询. 相似文献
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结合目前数字测图一体化发展的需求,对超站仪和PDA相结合的作业方式进行了分析,给出了这种模式的实现方法,对进一步提高外业测图工作效率,减轻作业人员负担,使外业数字测图更加方便快捷具有重要意义。 相似文献
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分析了目前常用的空间索引算法的缺点,提出了一种利用Windows API 提供的区域、路径、裁剪操作来建立网格空间索引的算法,对比实例证明,其具有简单易行,构建网格索引效率高、精度高等特点. 相似文献
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