Isolation and characterization of 49 polymorphic microsatellite loci for Decapterus maruadsi using SLAF-seq,and cross-amplification to related species

Decapterus maruadsi is a commercially important species in China, but has been heavily exploited in some areas. There is a growing need to develop microsatellites promoting its genetic research for the adequate management of this fishery resources. The recently developed specific-locus amplified fragment sequencing (SLAF-seq) is an efficient and high-resolution method for genome-wide microsatellite markers discovery. In this study, 28 905 microsatellites (mono- to hexa-nucleotide repeats) were identified using SLAF-seq technology, of which di-nucleotide was the most frequent (13 590, 47.02%), followed by mono-nucleotide (8 138, 28.15%), tri-nucleotide (5 727, 19.81%), tetra-nucleotide (1 104, 3.82%), pentanucleotide (234, 0.81%), and hexa-nucleotide (112, 0.39%). One hundred and thirty-two microsatellite loci (di- and tri-nucleotide) were randomly selected for amplification and polymorphism, of which 49 were highly polymorphic and well-resolved. The average number of alleles per locus was 13.63, ranging from 4 to 25, and allele sizes varied between 110 bp and 309 bp. The observed heterozygosity ( H_o ) and expected heterozygosity ( H_e ) ranged from 0.233 to 1.000 and from 0.374 to 0.959, with mean values of 0.738 and 0.836, respectively. The polymorphism information content (PIC) ranged from 0.341 to 0.941 (mean=0.806). However, 12 loci deviated from Hardy-Weinberg equilibrium. Furthermore, transferability tests were also successful in validating the utility of the developed markers in five phylogenetically related species of family Carangidae. A total of 48 microsatellite markers were successfully cross-amplified in Decapterus macarellus, Decapterus macrosoma, Decapterus kurroides, Trachurus japonicus, and Selaroides leptolepis. The present microsatellites provided the first known set of microsatellite DNA markers for D. maruadsi, D. macarellus, D. kurroides, and D. macrosoma, and would be useful for further population genetic and molecular phylogeny studies as well as help with the fisheries management formulation and implementation of the understudied species.

     

The conservation patterns of grassland ecosystem in response to the forage-livestock balance in North China

Being a key ecological security barrier and production base for grassland animal husbandry in China,the balance between grassland forage supply and livestock-carrying pressure in North China directly affects grassland degradation and restoration,thereby impacting grassland ecosystem services.This paper analyzes the spatiotemporal variation in grassland vegetation coverage,forage supply,and the balance between grassland forage supply and livestock-carrying pressure from 2000 to 2015 in North China.We then discuss the spatial pattern of grassland ecological conservation under the impacts of grassland degradation and restoration,and livestock-carrying pressure.Over the last 16 years,the total grassland area in North China decreased by about 16,000 km2,with vegetation coverage degraded by 6.7％ of the grasslands but significantly restored by another 5.4％ of grasslands.The provisioning of forage by natural grassland mainly increased over time,with an annual growth rate of approximately 0.3 kg/ha,but livestock-carrying pressure also increased continuously.The livestock-carrying pressure index without any supplementary feeding reached as high as 3.8.Apart from the potential livestock-carrying capacity in northeastern Inner Mongolia and the central Tibetan Plateau,most regions in North China are currently overloaded.Considering the actual supplementary feeding during the cold season,the livestock-carrying pressure index is about 3.1,with the livestock-carrying pressure mitigated in central and eastern Inner Mongolia.Assuming full supplementary feeding in the cold season,livestock-carrying pressure index will fall to 1.9,with the livestock-carrying pressure alleviated significantly in Inner Mongolia and on the Tibetan Plateau.Finally,we propose different conservation and development strategies to balance grassland ecological conservation and animal husbandry production in different regions of protected areas,pastoral areas,farming-pastoral ecotone,and farming areas,according to the grassland ecological protection patterns.     

经济聚集与产业结构对城市土地利用效率的影响

<中作者单位三>=河南大学 环境与规划学院,河南 开封 475004从经济集聚、产业结构两方面构建城市土地利用效率影响因素分析框架,并通过计量经济模型定量考察经济集聚、产业结构对城市土地利用效率的影响.结果表明:① 现阶段中国城市土地利用效率处于随集聚程度的提高而增加的阶段,拥挤效应还不明显.同时,集聚程度的不同方面对土地利用效率的贡献大小存在差异,资本密度对城市土地利用效率的影响大于就业密度和人口规模,这表明现阶段中国城市经济增长主要由资本投入推动的.② 城市产业结构优化能够提高土地利用效率.资本有机构成的提高,意味着资本对劳动的替代作用增强,其进一步加大了资本对城市土地产出的推动作用,有利于土地利用效率的提升,但不利于城市就业水平的提升.     

Comparison of instrumental and interpolated meteorological data-based summer temperature reconstructions on Mt. Taibai in the Qinling Mountains,northwestern China

Theoretical and Applied Climatology - Previous dendroclimatical studies have been based on the relationship between tree growth and instrumental climate data recorded at lower land meteorological...     

厄尔尼诺对长江中下游地区夏季持续性降水结构的影响及其可能机理

根据中国国家气象信息中心提供的1961-2016年2400多站的逐日降水观测数据,分析了厄尔尼诺对长江中下游地区夏季持续性降水结构的影响。发现长江中下游地区的降水主要以5 d及以内的降水事件为主,其强度主要分布在4-24 mm/d;5 d以上降水所占比例相对较小,而其强度主要分布在12-24 mm/d。其中,长江中下游地区夏季大于5 d的降水事件（长持续性降水事件）所占比例和强度在长江以南地区要大于长江以北地区。同时,在厄尔尼诺的影响下,长江以南地区的降水结构从2-5 d持续性降水事件（短持续性降水事件）和1 d的降水事件（非持续性降水事件）向长持续性降水事件转变,且其强度增加。而长江以北地区,以湖北为主,降水结构存在从非持续性向短持续性降水事件转变的现象,短持续性降水事件的强度也略有增强。因此,厄尔尼诺使得长江中下游地区的降水事件更多地以持续性降水为主,不同持续性降水事件的强度加强。进一步分析发现厄尔尼诺次年西太平洋副热带高压西伸加强,与其相关的东南季风所输送的水汽也有所加强。同时,中高纬度阻塞高压环流形势稳定维持。受这些因子的共同作用,最终导致长江中下游地区夏季降水持续性延长和降水强度加强。而这将给长江中下游地区的农作物种植和经济发展等带来较严重的影响,使防洪、防涝工作面临严峻的挑战。      

优化K means算法在中国近海气候区划中的应用

利用我国近海多年的风、浪及温湿资料,采用DB指标和最大距离法相结合的优化K-means聚类算法,对冬季我国近海进行气候区划,避免了传统K-means算法中确定聚类数目和初始聚类中心的主观性。结果表明,冬季我国近海可被划分为3个区域:1区主要包括28°N以北的海域以及我国东南沿海的一条狭长区域,2区主要包括台湾周边海域、台湾海峡、巴士海峡以及自越南东南部起由西南部向东北部的南海大部分海域,3区则主要包括台湾岛西南部海域、北部湾、广东沿海以及南海东南部。根据给出的区划指标,分析各分区的气候特征及其对海上舰船航行和出海人员的影响,判定1区为基本适宜区,2区为不适宜区,3区为适宜区。     

2011—2015年安徽省高速公路低能见度变化特征

利用2011—2015年安徽省高速公路自动气象观测站观测的能见度资料,分析了安徽省低能见度年、日变化及其与降水的关系,结果表明：受降雨影响,大别山区和皖南山区部分站点低能见度出现频率最高时段在汛期（5—9月）,其他地区主要出现在10月和11月。低能见度出现时间存在明显日变化,夜间出现频率高于白天,19：00以后开始逐渐增多,日出前后达到最大,之后迅速减少。7—8月的13：00—17：00出现与降水相关的低能见度频率较高。不同季节里,各站低能见度出现最多的时间和频率存在一定差异,春季和秋季最低能见度主要出现在日出前后,冬季主要出现在夜间到日出之前,夏季江淮之间北部和淮北地区主要出现在日出前后,江淮之间南部到江南在一天各时间段内出现频率较为均匀。     

基于环境因素约束的农户土地利用效率及影响因素分析 ——以河南省粮食生产核心区为例

在农户土地利用引起的环境问题日益严重的背景下,如果农户土地利用效率的测度只考虑“好”产出,而忽略“非意欲”的环境产出,可能造成测度结果不准确。因此,将“非意欲”产出引入农户土地利用效率评价模型,利用河南省粮食生产核心区农户抽样调查数据测度环境因素约束下的农户土地利用效率,并探讨其效率状况及影响因素。主要的结论如下：① 调查区域环境因素约束下的农户土地利用效率平均值仅为0.612,效率值总体不高,有较大的提升空间。不同规模农户的土地利用效率差异较大,二者呈现“U型”变化趋势。② 环境因素约束下的农户土地利用效率的影响因素来自多个方面。其中,农户生计方式兼业化在一定程度上降低了环境约束下的农户土地利用效率;种植结构调整虽然能够增加农户农业收入,但不利于环境因素约束下的农户土地利用效率的提升;农地经营规模扩大对环境约束下的农户土地利用效率产生正面影响,但现实中普遍存在的小规模化经营和土地细碎化消减了正面影响。土地产权的稳定性能够提升环境约束下的农户土地利用效率,但无论是集体土地产权还是承包经营权以及农户间非正式的土地流转产权关系稳定性较差,不利于环境约束下的农户土地利用效率的提高。     

An integrated technology for gas control and green mining in deep mines based on ultra-thin seam mining

With the increasing demand for coal resources, coal mining has gradually entered into the deep strata of coal seams. Although the increase in mining depth improves energy security, it is associated with severe hazards, especially coal and gas outburst. Protective seam mining is an efficient method for gas control and has been widely used in major coal-producing countries. However, studies on deep ultra-thin protective seam (thickness 0.1–1 m, average thickness 0.5 m) mining and its related problems have been rarely reported. Focusing on the challenges resulting from deep mining (mining depth >1100 m) and the research gap, a coal and gas co-exploitation technique, which combines the gas control technology and green mining (including coal preparation and backfilling), has been proposed in this work. Significant benefits have been achieved in the twelfth coal mine of the Pingdingshan coalfield (study area) following the implementation of this technique. The application of the gas control technology markedly improved the gas drainage efficiency, promoted increased gas utilization, and reduced the greenhouse gas emission, providing notable economic and environmental benefits. In addition, implementation of green mining improved the coal quality, relieved the burden of the transport system, and, in particular, effectively prevented surface subsidence, thus protecting the ecological environment of the mining area, which offered significant economic, environmental, and social benefits. The practice in the twelfth coal mine could be used as a valuable example for coal mines with similar geological conditions.     

准噶尔盆地古隆起的分布与基本特征

准噶尔盆地外侧三大边界动力体系的作用及基底结构的差异性导致准噶尔盆地在不同地质时期发育不同性质的隆起构造。石炭纪晚期东北侧的造山作用形成了盆地北部隆起,晚二叠世北部隆起分异为陆梁隆起与乌伦古坳陷;早二叠世西北侧的造山作用形成了车排子隆起,随后的冲断作用形成了克拉玛依--夏子街断裂带。燕山期的压扭作用形成了沉积盖层中的车排子--莫索湾隆起。新生代北天山向北的冲断作用导致准噶尔盆地南部地区急剧挠曲沉降,盆地向南掀斜,隆起向北迁移。据基底的性质、形成时间、活动方式、地质结构与保存状态等特点将准噶尔盆地的隆起划分为继承型、间断型、掀斜型与冲断带型等4种基本类型。