Cadmium-binding proteins in the blue crab, callinectes sapidus: laboratory-field comparison

The blue crab, Callinectes sapidus, is distributed along the east coast of the United States from Cape Cod, Mass., through the Gulf of Mexico, including both relatively unpolluted coastal areas and estuaries contaminated with trace metals. Cadmium is of particular concern because it is concentrated in the digestive glands of blue crabs and can be passed on to consumer organisms. Tissue concentrations and partitioning of trace metals from crabs exposed in the laboratory to 10 ppb dissolved cadmium for 40 days were compared with blue crabs collected from two locations on the Hudson River, NY, Foundry Cove and Haverstraw Bay, Foundry Cove and Haverstraw Bay, both of which have elevated trace metal levels relative to estuarine areas near Beaufort, NC. Crab digestive glands, gills and muscle were removed and analyzed for total cadmium, copper, zinc and nickel concentrations using acid digestion and atomic absorption spectrophotometry, and metal-binding (metallothionein-like) proteins were determined by gel-filtration chromatography. In crabs exposed to cadmium in the laboratory, the cytosolic partitioning was similar to previous investigations at our laboratory where higher levels of cadmium (100 ppb) and shorter exposure times (14 days) were used. The similarity in cadmium partitioning from these two separate experiments indicates dose independence. In crabs from polluted environments the digestive glands contained the highest concentrations of trace metals. Chromatograms of the cytosol from the digestive glands and gills from both field and laboratory exposed crabs showed similar distributions of cadmium, copper and zinc. The gills of both field and laboratory-exposed crabs had metal-binding proteins that contained mostly cadmium, and the digestive glands had metallothionein-like proteins that contained cadmium, copper and zinc. Estimated molecular weights for these proteins were similar to the metallothioneins found in other crustaceans and mammals.     

Retrieval of effective cloud field parameters from radiometric data

Theoretical and Applied Climatology - This study presents evaluation of the ability of Rossby Centre Regional Climate Model (RCA4) driven by nine global circulation models (GCMs), to skilfully...     

What Ångström—Prescott equation tells us about the cloud and clear-sky climatologies?

Theoretical and Applied Climatology - The Ångström–Prescott equation defines generically the relationship between solar energy available at ground level and sunshine duration. From...     

Heterogeneity in hedonic modelling of house prices: looking at buyers’ household profiles

This paper introduces household-level data into hedonic models in order to measure the heterogeneity of implicit prices regarding household type, age, educational attainment, income, and the previous tenure status of the buyers. Two methods are used for this purpose: a first series of models uses expansion terms, whereas a second series applies Geographically Weighted Regressions. Both methods yield conclusive results, showing that the marginal value given to certain property specifics and location attributes do vary regarding the characteristics of the buyer’s household. Particularly, major findings concern the significant effect of income on the location rent as well as the premium paid by highly-educated households in order to fulfil social homogeneity.     

A simple gibbsite analyzer for rapid field determination of the available alumina content in bauxites

A portable gibbsite analyzer suited for field operation and intended for rapid determination of available alumina is described. The available alumina content is calculated from the relative weight loss caused by the dehydration of gibbsite. More than 100 samples of gibbsite-containing bauxites of different origin, were analyzed with this apparatus. The results always appeared to be correct within 10% of the actual content. In the laboratory, an accuracy of ±2.60% gibbsite, and a reproducibility of ±1.87% gibbsite, both at the 95% confidence interval, could be achieved. The apparatus can be used under tropical conditions. Its weight is 13 kg. It can be fed from a motor-driven generator weighing 18 kg. The duration of a single analysis in the field is 30–40 minutes. In the laboratory about 25 analyses can be performed within 8 hours. The apparatus can be handled by an untrained operator. One operator can run at least two analyzers at a time. The complete set-up, including the generator, costs less than US $ 1500.

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Zusammenfassung Ein tragbarer Apparat für die Gibbsit Bestimmung im Freien wird beschrieben. Der Gibbsitgehalt wird berechnet aus dem Gewichtsverlust während der Entwässerung. Für Gibbsit-haltige Bauxite, die mit diesem Apparat analysiert sind, wurden Resultate gefunden, die immer weniger als 10% vom richtigen Gehalt abwichen. Im Labor konnte eine Genauigkeit von ±2.60% Gibbsit und eine Reproduzierbarkeit von ±1.87% Gibbsit, beide mit einer Wahrscheinlichkeit von 95%, erreicht werden. Der Apparat ist ausgerüstet für den Gebrauch in den Tropen. Er wiegt 13 kg und kann von einem durch einen Benzinmotor angetriebenen Generator gespeist werden. Der Generator hat ein Gewicht von 18 kg. Eine einzige Bestimmung im Freien dauert 30–40 Minuten. Im Labor können etwa 25 Bestimmungen innerhalb von 8 Stunden gemacht werden. Ein Mann kann mindestens zwei Apparate zugleich beobachten. Apparat und Generator kosteten weniger als US $ 1500.

     

Resolving frontal structures: on the payoff using a less diffusive but computationally more expensive advection scheme

This article presents some advantages using a shape-preserving total variation diminishing (TVD) advection scheme in an ecosystem model. The superbee flux-limiter has been used to the second-order Lax–Wendroff advection scheme to make it TVD. We performed simulations for three shelf sea regions with different characteristic time scales, namely, the North Sea, the Barents Sea, and the Baltic Sea. To explore the advantages, we also performed reference runs with the much simpler and computationally cheaper upwind advection scheme. Frontal structures are much better resolved with the TVD scheme. The bottom salinity in the Baltic Sea stays at realistic values throughout the 10-year simulation with the TVD scheme, while with the upwind scheme, it drifts towards lower values and the permanent haline stratification in the Baltic is almost completely eroded within one seasonal cycle. Only when applying TVD for both the vertical and horizontal advections the model succeeded to preserve haline stratification in the decadal simulation. Lower trophic level patterns are far better reproduced with the TVD scheme, and for the estimated cod larval survival, the advantages seem to be even stronger. Simulations using the TVD-derived prey fields identified distinct regions such as Dogger Bank to favor potential larvae survival (PLS), which did not appear as particularly favorable in the upstream simulations. The TVD scheme needs about 25?% more time on the central processing unit (CPU) in case of a pure hydrodynamic setup with only two scalar state variables (Barents Sea application). The additional CPU time cost increases for a coupled physical–biological model application with a large number of state variables. However, this is more than compensated by all the advantages found, and, hence, we conclude that it is worthwhile to use the TVD scheme in our ecosystem model.     

The Small Unmanned Meteorological Observer SUMO: Recent developments and applications of a micro-UAS for atmospheric boundary layer research

During the last 5 years, the Small Unmanned Meteorological Observer SUMO has been developed as a flexible tool for atmospheric boundary layer (ABL) research to be operated as sounding system for the lowest 4 km of the atmosphere. Recently two main technical improvements have been accomplished. The integration of an inertial measurement unit (IMU) into the Paparazzi autopilot system has expanded the environmental conditions for SUMO operation. The implementation of a 5-hole probe for determining the 3D flow vector with 100 Hz resolution and a faster temperature sensor has enhanced the measurement capabilities.     

Estimating aquifer channel recharge using optical data interpretation

Recharge through intermittent and ephemeral stream channels is believed to be a primary aquifer recharge process in arid and semiarid environments. The intermittent nature of precipitation and flow events in these channels, and their often remote locations, makes direct flow and loss measurements difficult and expensive. Airborne and satellite optical images were interpreted to evaluate aquifer recharge due to stream losses on the Frio River in south-central Texas. Losses in the Frio River are believed to be a major contributor of recharge to the Edwards Aquifer. The results of this work indicate that interpretation of readily available remote sensing optical images can offer important insights into the spatial distribution of aquifer recharge from losing streams. In cases where upstream gauging data are available, simple visual analysis of the length of the flowing reach downstream from the gauging station can be used to estimate channel losses. In the case of the Frio River, the rate of channel loss estimated from the length of the flowing reach at low flows was about half of the loss rate calculated from in-stream gain-loss measurements. Analysis based on water-surface width and channel slope indicated that losses were mainly in a reach downstream of the mapped recharge zone. The analysis based on water-surface width, however, did not indicate that this method could yield accurate estimates of actual flow in pool and riffle streams, such as the Frio River and similar rivers draining the Edwards Plateau.     

Response of braiding channel morphodynamics to peak discharge changes in the Upper Yellow River

The braiding intensity and dynamics in large braiding rivers are well known to depend on peak discharges, but the response in braiding and channel–floodplain transformations to changes in discharge regime are poorly known. This modelling study addresses the morphodynamic effects of increasing annual peak discharges in braiding rivers. The study site is a braiding reach of the Upper Yellow River. We estimated the effects on the larger‐scale channel pattern, and on the smaller‐scale bars, channel branches and floodplains. Furthermore, we determined the sensitivity of the channel pattern to model input parameters. The results showed that the dominant effect of a higher peak discharge is the development of chute channels on the floodplains, formed by connecting head‐cut channels and avulsive channels. Widening of the main channel by bank erosion was found to be less dominant. In addition, sedimentation on the bars and floodplains increased with increasing peak discharge. The model results also showed that the modelled channel pattern is especially sensitive to parametrization of the bed slope effect, whereas the effect of median grain size was found to be relatively small. Copyright © 2018 John Wiley & Sons, Ltd.     

Assessing the use of global land cover data for guiding large area population distribution modelling

Gridded population distribution data are finding increasing use in a wide range of fields, including resource allocation, disease burden estimation and climate change impact assessment. Land cover information can be used in combination with detailed settlement extents to redistribute aggregated census counts to improve the accuracy of national-scale gridded population data. In East Africa, such analyses have been done using regional land cover data, thus restricting application of the approach to this region. If gridded population data are to be improved across Africa, an alternative, consistent and comparable source of land cover data is required. Here these analyses were repeated for Kenya using four continent-wide land cover datasets combined with detailed settlement extents and accuracies were assessed against detailed census data. The aim was to identify the large area land cover dataset that, combined with detailed settlement extents, produce the most accurate population distribution data. The effectiveness of the population distribution modelling procedures in the absence of high resolution census data was evaluated, as was the extrapolation ability of population densities between different regions. Results showed that the use of the GlobCover dataset refined with detailed settlement extents provided significantly more accurate gridded population data compared to the use of refined AVHRR-derived, MODIS-derived and GLC2000 land cover datasets. This study supports the hypothesis that land cover information is important for improving population distribution model accuracies, particularly in countries where only coarse resolution census data are available. Obtaining high resolution census data must however remain the priority. With its higher spatial resolution and its more recent data acquisition, the GlobCover dataset was found as the most valuable resource to use in combination with detailed settlement extents for the production of gridded population datasets across large areas.