Electrochemical detection of harmful algae and other microbial contaminants in coastal waters using hand-held biosensors

Standard methods to identify microbial contaminants in the environment are slow, laborious, and can require specialized expertise. This study investigated electrochemical detection of microbial contaminants using commercially available, hand-held instruments. Electrochemical assays were developed for a red tide dinoflagellate (Karenia brevis), fecal-indicating bacteria (Enterococcus spp.), markers indicative of human sources of fecal pollution (human cluster Bacteroides and the esp gene of Enterococcus faecium), bacterial pathogens (Escherichia coli 0157:H7, Salmonella spp., Campylobacter jejuni, Staphylococcus aureus), and a viral pathogen (adenovirus). For K. brevis, two assay formats (Rapid PCR-Detect and Hybrid PCR-Detect) were tested and both provided detection limits of 10 genome equivalents for DNA isolated from K. brevis culture and amplified by PCR. Sensitivity with coastal water samples was sufficient to detect K. brevis that was "present" (相似文献   

ESR dating of tooth enamel in Mousterian layer 20, El Castillo,Spain

Nine faunal teeth from layer 20 of El Castillo cave in Cantabrian Spain were dated using electron spin resonance (ESR). Two teeth were rejected due to inconsistent subsample ages, while the remaining teeth yielded a mean age that is consistent with the stratigraphic expectations: 42.7±3.5. Uncertainty in the external γ dose rate results in a potential systematic uncertainty of±6.4ka that should affect all samples equally. The results provide independent confirmation of previously reported ¹⁴C ages for layer 20. © 2010 Wiley Periodicals, Inc.     

Cloud computing and virtualization within the regional climate model and evaluation system

The Regional Climate Model Evaluation System (RCMES) facilitates the rapid, flexible inclusion of NASA observations into climate model evaluations. RCMES provides two fundamental components. A database (RCMED) is a scalable point-oriented cloud database used to elastically store remote sensing observations and to make them available using a space time query interface. The analysis toolkit (RCMET) is a Python-based toolkit that can be delivered as a cloud virtual machine, or as an installer package deployed using Python Buildout to users in order to allow for temporal and spatial regridding, metrics calculation (RMSE, bias, PDFs, etc.) and end-user visualization. RCMET is available to users in an “offline”, lone scientist mode based on a virtual machine dynamically constructed with model outputs and observations to evaluate; or on an institution’s computational cluster seated close to the observations and model outputs. We have leveraged RCMES within the content of the Coordinated Regional Downscaling Experiment (CORDEX) project, working with the University of Cape Town and other institutions to compare the model output to NASA remote sensing data; in addition we are also working with the North American Regional Climate Change Assessment Program (NARCCAP). In this paper we explain the contribution of cloud computing to RCMES’s specifically describing studies of various cloud databases we evaluated for RCMED, and virtualization toolkits for RCMET, and their potential strengths in delivering user-created dynamic regional climate model evaluation virtual machines for our users.     

The age of the Roter Kamm impact crater,Namibia: Constraints from 40Ar-39Ar,K-Ar,Rb-Sr,fission track,and 10Be-26Al studies

Abstract The well-preserved 2.5 km diameter Roter Kamm impact crater is located in the Namib desert in Namibia. The impact has occurred in Precambrian granitic and granodioritic orthogneisses of the 1200–900 Ma old Namaqualand Metamorphic Complex which were partly covered by Gariep metasediments; the granites are invaded by quartz veins and quartz-feldspar-pegmatites. Previous geological field evidence suggested a crater age of about 5–10 Ma. In order to constrain this age, we selected a set of basement rocks (granites, granodiorites) exposed at the crater rim and studied the Rb-Sr, K-Ar, ⁴⁰Ar-³⁹Ar, and ¹⁰Be-²⁶Al isotopic systems as well as apatite fission track ages of these samples. The Rb-Sr isotopic systematics confirm the derivation of these samples from the Namaqualand basement (age about 1.29 Ga), which underwent Damaran orogenesis at about 650 Ma. No basement rocks with Rb-Sr ages younger than about 410 Ma were identified. The K-Ar ages of pseudotachylite and melt breccia samples show that these samples are dominated by incompletely degassed fragments of basement rocks, with some retaining their original metamorphic ages of about 470 Ma. The apatite fission track ages range from 20–28 Ma, which may be interpreted as an extension of the 25 Ma Burdigalian peneplanation event, or as incomplete resetting of the apatite fission tracks during the impact event. The ¹⁰Be and ²⁶Al exposure age of a quartz sample isolated from a quartz-pegmatite was found to be 150 ka; it is likely that the exposure of the sample began after material covering it had been removed by erosion 150 ka ago. Two glassy fractions extracted from a rim granite were dated by ⁴⁰Ar-³⁹Ar analysis. One sample gives practically a plateau age of 3.7 ± 0.3 Ma, while the other gives a minimum age of 3.6 Ma. The best available age estimate for the Roter Kamm crater is therefore 3.7 ± 0.3 Ma.     

New 1982 — 1990 photometry of λ Andromedae and its 11-year cycle

We present photoelectric photometry of λ And never before published, obtained between February 1982 and December 1990 at 29 different observatories. Then we combine it with all other photometry available to us (previously published, contained in the I.A.U. Commission 27 Archives, and obtained with the Vanderbilt 16-inch automatic telescope but not yet published), to yield a 14.8-year data base. Analysis reveals a long-term cycle in mean brightness, with a full range of 0^m.15 and a period of 11.4 ± 0.4 years. Because most of our new photometry was concentrated in the 1983-84 observing season, we analyze that one well-defined light curve with a two-spot model. Spot A keeps a 0^m.04 amplitude throughout four rotation cycles whereas the amplitude of spot B diminishes from 0^m.09 down almost to 0^m.03. The spot rotation periods were 55^d.9 ± 0^d.6 and 520^d.8 ± 1^d.0, respectively.     

Searching for nanoflares

The evolution of hydrodynamic shocks generated by nanoflares in coronal loops is investigated in order to suggest requirements for future observations. The shocks produced by nanoflares of 10²⁴ ergs decay within a few seconds, which sets stringent requirements for Doppler or transverse velocity measurements. With feasible improvements, the Normal Incidence X-ray Telescope could observe the time-averaged emission from a nanoflare, however. We also consider collisions between shocks and show that these could also be observed.Operated by the Association of Universities for Research in Astronomy (AURA) under agreement with the Natinal Science Foundation.     

In search of the Australasian tektite source crater: The Tonle Sap hypothesis

Abstract— The source crater for Australasian tektites remains to be positively identified We suggest that Tonle Sap, a roughly oval lake in south-central Cambodia, may represent the remnant of that crater. The size of the lake (about 100 km × 35 km), location (Indochina), inferred geologic age (recent), and orientation of the lake, as well as the geographical distribution of tektites, are consistent with this suggestion. The elongated shape of the lake with its long axis pointing toward Australia may be the result of an oblique impact of a NW to SE-moving object a few km in diameter. The absence of a raised rim and a central peak may be related to a low impact angle, soft target rocks, or high post-impact erosion and sedimentation rates. The scarcity of Muong Nong-type (layered) tektites near Tonle Sap may be due to extensive post-impact alluvial deposition, which buried the tektites. The chemical composition of Upper Indosinias formation sandstones from Phnom Batheay was determined. There are significant differences between the composition of indochinite tektites and these rocks, which are thus unlikely to represent tektite source rocks.     

Flows in quiescent prominences

Earlier studies of quiescent prominences claim that there is a systematic downward directed motion of the small-scale structure. Disk observations, on the other hand, have detected mass motions both upwards and downwards. The earlier high-resolution observations of limb prominences have been re-examined using local cross-correlation techniques for measurements of motion perpendicular to the line of sight. The new measurements reveal flow speeds and directions that are in good agreement with current Doppler measurements on the disk.     

Fe and O isotope composition of meteorite fusion crusts: Possible natural analogues to chondrule formation?

Meteorite fusion crust formation is a brief event in a high‐temperature (2000–12,000 K) and high‐pressure (2–5 MPa) regime. We studied fusion crusts and bulk samples of 10 ordinary chondrite falls and 10 ordinary chondrite finds. The fusion crusts show a typical layering and most contain vesicles. All fusion crusts are enriched in heavy Fe isotopes, with δ⁵⁶Fe values up to +0.35‰ relative to the solar system mean. On average, the δ⁵⁶Fe of fusion crusts from finds is +0.23‰, which is 0.08‰ higher than the average from falls (+0.15‰). Higher δ⁵⁶Fe in fusion crusts of finds correlate with bulk chondrite enrichments in mobile elements such as Ba and Sr. The δ⁵⁶Fe signature of meteorite fusion crusts was produced by two processes (1) evaporation during atmospheric entry and (2) terrestrial weathering. Fusion crusts have either the same or higher δ¹⁸O (0.9–1.5‰) than their host chondrites, and the same is true for Δ¹⁷O. The differences in bulk chondrite and fusion crust oxygen isotope composition are explained by exchange of oxygen between the molten surface of the meteorites with the atmosphere and weathering. Meteorite fusion crust formation is qualitatively similar to conditions of chondrule formation. Therefore, fusion crusts may, at least to some extent, serve as a natural analogue to chondrule formation processes. Meteorite fusion crust and chondrules exhibit a similar extent of Fe isotope fractionation, supporting the idea that the Fe isotope signature of chondrules was established in a high‐pressure environment that prevented large isotope fractionations. The exchange of O between a chondrule melt and an ¹⁶O‐poor nebula as the cause for the observed nonmass dependent O isotope compositions in chondrules is supported by the same process, although to a much lower extent, in meteorite fusion crusts.