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991.
992.
利用RAPD和AFLP标记 ,以一回交家系 [(Miyagi×Hiroshima)×Miyagi]为作图群体 ,构建了太平洋牡蛎的遗传连锁图谱。用经过筛选的 3 3个RAPD引物和 1 1个AFLP引物组合 ,对父母本和 80个子代个体进行了遗传分析。共得到母本分离标记 1 93个 ,其中 1 44个符合1∶1孟德尔分离规律。父本分离标记 1 5 6个 ,其中 1 1 1个符合 1∶1孟德尔分离规律。雌性框架图包括 99个遗传标记 ,定位在 1 2个连锁群中 ,覆盖 985 2cM ,标记间平均间隔 1 1 3cM。另外有 3个三联体 ,7个连锁对 ,图谱共覆盖 1 1 6 5 7cM。雄性框架图包括 72个遗传标记 ,分布在 8个连锁群 ,覆盖 81 1cM ,标记间平均间隔 1 2 7cM。另外有 4个三联体 ,3个连锁对 ,图谱共覆盖93 1 8cM。 相似文献
993.
Diagnosis of iridovirus in large yellow croaker by PCR 总被引:1,自引:0,他引:1
A rapid and sensitive PCR-based method for the detection of the large yellow croaker iridovirus(LYCIV) is described,which involves the amplification of a 295 bp fragment of the LYCIV ATPase gene from DNA isolated from naturally infected fish spleen.Sequencing of LYCIV ATPase gene fragment showed it shared 100% nucleotide sequence homology with the corresponding region of the ATPase gene of red sea bream iridovirus(RSIV) and sea bass iridovirus(SBIV),suggesting that LYCIV was homologous with RSIV and SBIV at least in part of the gemone.The specificity and sensitivity of the PCR procedure were tested on the iridovirus-infected fishes,the expected fragment was detected from spleen DNA samples of infected fishes,whereas no fragments were amplified from healthy fish spleen DNA,white spot syndrome baculoviruses(WSBV) DNA and pseudorabies virus(PRV) DNA.Detection limit of this method was 10-7 ng positive plasmid DNA containing target sequence,equal to about 100 virions.In the infected experiment,first positive detection(1/4) appeared at Day 3 post-infection,all fish(4/4) tested positive at Day 7,however obvious symptoms were observed at Day 8,so LYCIV infection could be detected prior to the appearance of obvious symptoms.These results indicate that this PCR method could be used for early,rapid and specific detection of LYCIV infection. 相似文献
994.
水成沉积与风成沉积及古土壤的磁组构特征 总被引:14,自引:2,他引:14
晚第四纪水成沉积、风成沉积、古土壤等的磁组构研究表明,不同成因的沉积物具有明显不同的磁组构特征。一般地,水成沉积的体积磁化率K受物源和水动力因素控制,P和F均>1.02,q<0.5;风成沉积的K受物源控制,内陆黄土的K明显高于沿海黄土及海岸风沙K,P和F均<1.02,q的平均值>0.55;古土壤的K受母质沉积类型和成土环境的氧化还原条件控制,内陆古土壤的K偏高,沿海古土壤的K偏低,内陆古土壤的K远高于沿海古土壤的K,P和F均<1.02,q的平均值在0.5左右。运用典型沉积的磁组构特征对比分析未知沉积物,可以得到未知沉积物的物源、沉积过程和环境信息。 相似文献
995.
Observations of fluid mud were made in the lower North Passage of the Yangtze Estuary in February 2000, on 10 -11 August 2000, on 30 - 31 August 2000 (after two strong typhoons), on 21 - 24 August 2000 (neap tide) and on 3 -6 September 2000 (mean tide) respectively. In situ data show that the fluid mud in this area consists of fine cohesive sediment (median size 7.23 μm). The formation and movement of fluid mud varied during the neap-spring and flood-ebb tidal cycle. Observations suggest that fluid mud phenomena in this area may be categorised in a three-fold manner as slack water, storm and saltwedge features. The thickness of the fluid mud layer of slack water during the neap tide ranged from 0.2 to 0.96 m, whereas during the mean tide, the thickness ranged from 0.17 to 0.73 m, and the thickness of the fluid mud layer was larger during slack water than at the flood peak. Shoals cover an area of 800 km^2 with a water depth smaller than 5 m. Erosion of these extensive intertidal mudflats due to storm action provides an abundant sediment source. This is particularly significant in this estuary when the tidal level is lower than 5 m. The lower North Passage is a typical zone of saltwater wedging, so the saltwedge fluid mud has the most extensive spatial range in the estuary. 相似文献
996.
997.
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999.
The Barra Nova inlet, in south Portugal, is known to migrate progressively southeastwards under wave action. The morphodynamics
of this system during a representative year suggests that this long-term evolution is dependent on a seasonal behavior of
the tidal inlet which can be described through a three-stage model of post-storm, transition and extended fair-weather conditions.
Processes involved in this evolution indicate that the historical migration of the Barra Nova is not dependent on the longshore
drift forcing constricting the channel on the updrift coast, but rather on the adjustment of the system to a major erosion
of the downdrift coast during short storm events. 相似文献
1000.