Identification and characterization of the Vibrio anguillarum prtV gene encoding a new metalloprotease

We cloned and sequenced a prtV-like gene from Vibrio anguillarum M3 strain. This prtV gene encodes a putative protein of 918 amino acids, and is highly homologous to the V. cholerae prtV gene. We found that a prtV insertion mutant strain displayed lower gelatinase activity on gelatin agar, lower protease activity against azocasein, and lower activity for four glycosidases. This prtV mutant strain also had increased activity for two esterases in its extracellular products, as analyzed by the API ZYM system. In addition, the prtV mutant strain exhibited decreased growth in turbot intestinal mucus and reduced hemolytic activity on turbot erythrocytes. Infection experiments showed that the LD₅₀ of the prtV mutant strain increased by at least 1 log compared to the wild-type in turbot fish. We propose that prtV plays an important role in the pathogenesis of V. anguillarum.     

沙漠公路风蚀破坏规律的数值模拟研究

通过Fluent软件对沙漠公路的风蚀破坏规律进行数值模拟研究。分析公路不同横断面时风沙流扰动、增速、减速、恢复的过程,确定路基(堑)高度、边坡坡率和路面宽度等公路断面设计参数对风沙流扰动的影响,提出沙漠公路路基(堑)风蚀破坏规律。结果表明：路面宽度的改变对路基沿程风速变化影响较小,各特征点处风速变化小于5%。路基(堑)高度和边坡坡率对风沙流扰动的影响较大。随路基(堑)高度增加,风沙流流场的扰动被增强,路基(堑)风蚀破坏越显著,当边坡坡率为1:1时,路基模型高度250 mm时的迎风路肩风速为模型高度60 mm时的 1.15倍,其背风坡脚风速降低45%;路堑模型高度250 mm时的背风堑顶风速为模型高度60 mm时的1.05倍,迎风堑脚处风速降低高达80%。建议沙漠公路宜采用中低路基(堑),不宜高填深挖。当路基(堑)高度一定,边坡坡率为1:1.75或1:2时,路基(堑)沿程风速变化平缓,沙漠路肩不易被吹蚀破坏;路堑中堑脚位置处不易出现堆积。其结论与室内风蚀风洞试验结果有很好的一致性     

鄯勒油田西山窑组层序地层格架及其油气地质意义

运用层序地层学的基本原理和技术方法,建立鄯勒油田西山窑组层序地层格架,划分沉积相,并在此基础上分析层序地层格架内砂体的展布特征和油气的聚集规律,预测研究区岩性油气藏的类型及分布。研究结果表明：西山窑组整体经历了一个先进积、后退积的过程;西山窑组为一个三级层序,其中包含2个四级层序,该组由低位域（LST）的三角洲前缘沉积、湖侵域（TST）的三角洲平原沉积和高位域（HST）的湖相沉积组成。研究区基准面上升期可以寻找砂体上倾尖灭型岩性圈闭,基准面下降期可以寻找砂岩透镜体岩性圈闭。西一、二段沉积的三角洲河道砂体受后期构造运动的影响,可以形成砂体上倾尖灭型岩性油气藏;而西三、四段的湖相浊积砂体,可以形成砂岩透镜体岩性油气藏。     

层状地基中空沟对交通荷载的竖向隔振分析

空沟是一种常用的连续隔振屏障,可以有效地降低人工振动。以位移为变量的二维格子法在波动问题计算中具有良好的适应性、高效性,兼备有限元与差分法的一些优点,应用该法针对层状地基中作用于路堤上的交通荷载引起振动的空沟竖向隔振问题进行了参数研究,分别考虑了上软下硬地基和上硬下软地基两种情况。研究结果表明：空沟宽度对隔振效果的影响较小;空沟深度与空沟的位置是影响隔振效果的关键因素,它们相互关联,其取值应综合考虑;在上述两类层状地基中,若空沟深度与上层土的厚度接近或相等均对竖向隔振不利;路堤高度越大对竖向隔振效果越不利。     

测井资料在储层预测研究中的应用

测井解释技术面临三方面制约因素:(1)长期以来仪器的发展速度快于解释评价的发展速度,使大量有用信息被长期掩盖;(2)现今测井评价方法强于微观解释而弱于宏观分析,明显限制了测井技术的发展和受重视程度;(3)油气勘探开发目标的日益复杂化,对测井技术提出了更高要求.开展测井储层预测技术的探索,在某种程度上可弥补上述不足.本文提出测井储层预测技术研究的二个依据,(1)将测井资料作为宏观地质作用与微观岩石信息统一关系论证的一个关键点,寻找测井信息能准确描述二者具有统一成因关系的证据;(2)以构造-沉积演化作为研究切入点,预测特殊储层在时、空的分布关系.基于上述认识,以印尼B油区裂缝型砂岩的预测以及大港油田Q50断块低阻油层的预测研究为例,分别介绍了测井储层预测技术的2个应用实例.     

Hydrologic alteration along the Middle and Upper East River (Dongjiang) basin, South China: a visually enhanced mining on the results of RVA method

This paper presents a visually enhanced evaluation of the spatio-temporal patterns of the dam-induced hydrologic alteration in the middle and upper East River, south China over 1952–2002, using the range of variability approach (RVA) and visualization package XmdvTool. The impacts of climate variability on hydrological processes have been removed for wet and dry periods, respectively, so that we focus on the impacts of human activities (i.e., dam construction). The results indicate that: (1) along the East River, dams have greatly altered the natural flow regime, range condition and spatial variability; (2) six most remarkable indicators of hydrologic alteration induced by dam-construction are rise rate (1.16), 3-day maximum (0.91), low pulse duration (0.88), January (0.80), July (0.80) and February (0.79) mean flow of the East River during 1952–2002; and (3) spatiotemporal hydrologic alterations are different among three stations along Easter River. Under the influence of dam construction in the upstream, the degree of hydrologic changes from Lingxia, Heyuan to Longchuan station increases. This study reveals that visualization techniques for high-dimensional hydrological datasets together with RVA are beneficial for detecting spatio-temporal hydrologic changes.     

Non-linear buffeting response analysis of long-span suspension bridges with central buckle

The rigid central buckle employed in the Runyang Suspension Bridge (RSB) was the first time it was used in a suspension bridge in China. By using a spectral representation method and FFT technique combined with measured data,a 3D fluctuating wind field considering the tower wind effect is simulated. A novel FE model for buffeting analysis is then presented,in which a specific user-defined Matrix27 element in ANSYS is employed to simulate the aeroelastic forces and its stiffness or damping matrices are parameterized by wind velocity and vibration frequency. A nonlinear time history analysis is carried out to study the influence of the rigid central buckle on the wind-induced buffeting response of a long-span suspension bridge. The results can be used as a reference for wind resistance design of long-span suspension bridges with a rigid central buckle in the future.     

Timing for high lake levels of Qinghai Lake in the Qinghai-Tibetan Plateau since the Last Interglaciation based on quartz OSL dating

Qinghai Lake is situated in the northeast of the Qinghai-Tibetan Plateau (QTP). Its size and proximity to the junction of three major climate systems make it sensitive to climate changes. Some investigations on shorelines of Qinghai Lake suggested highstands during MIS 3, but to what extent the lake level was higher than today is yet undetermined. Others proposed that the maximum highstands probably dated to MIS 5. It has also been shown that the lake level 120 m higher than today occurred at around 12 ka. Most of these previous ages were obtained using ¹⁴C dating or multiple-aliquot IRSL/OSL dating. For ¹⁴C dating, because of the dating limit (<40 ka) and the lack of suitable dating materials in this arid area, it is difficult to establish reliable chronological control. In the present study, seven samples collected from lacustrine deposits (five samples) and sand wedges (two samples) were dated using quartz optically stimulated luminescence (OSL) with the single aliquot regenerative-dose (SAR) protocol. OSL dating results showed that (1) the lake had experienced two high lake levels, one was in MIS 5 and another in early to middle MIS 3; (2) no evidence of high lake levels in MIS 4 has been found; (3) the alluvial gravels, whose surface is at an elevation of ～3246 m, were formed at least 28.8 ± 2.3 ka ago, and the widespread sand wedges within the alluvial gravels were formed during the period of 15.1–28.8 ka, which implied that the lake level had not reached an elevation of ～3240 m after 28.8 ± 2.3 ka.     

青藏高原东缘都江堰湖相沉积MIS3a阶段的快速气候变化记录

深海氧同位素第3阶段晚期(MIS3a)是青藏高原较为特殊的暖湿时期, 为了解这一时期高原东缘的气候变化细节, 利用AMS14C定年和粒度、总有机碳、正构烷烃等环境代用指标提取了四川都江堰湖相沉积剖面的相应记录.经过校正后的日历年龄显示此剖面沉积于43.6~34.6 kaBP期间, 记录了千年尺度的D-O旋回事件, 其分布时段分别为: DO11(43.6~41.8 kaBP), DO10(41.8~39.2 kaBP), DO9(39.2~37.7 kaBP), DO8(37.7~35.5 kaBP), DO7(35.5 kaBP~未见顶).该剖面总有机碳曲线与南京葫芦洞石笋和格陵兰冰心(GRIP)氧同位素曲线的对比表明, 该区域气候记录对全球高纬地区的冰量变化和亚洲季风演化具有响应; 而中纬度太阳辐射岁差对该区域气候波动的控制作用较强导致了细节上存在差异.      

鄂尔多斯盆地西部侏罗系煤储层特征及有利区预测

在系统采集样品的基础上,通过对煤样的煤质、煤岩显微组分、煤相、煤岩显微裂隙分析及孔隙度和压汞孔隙结构测试,研究了鄂尔多斯盆地西部的煤层气储层物性特征,并采用多层次模糊数学评价方法,预测了煤层气资源分布的有利区。结果表明,该区侏罗系煤储层具有较强的生气潜力和储气能力,孔隙系统具双峰分布特点,以微孔-小孔和大孔为主,其中二道岭-汝箕沟地区具备"小而肥"的资源优势,是该区煤层气勘探开发的最有利目标区。