Cloning and Characterization of Glyceraldehyde-3-phosphate Dehydrogenase Encoding Gene in Gracilaria/Gracilariopsis lemaneiformis

Glyceraldehyde-3-phosphate dehydrogenase （GAPDH） plays important roles in various cellular processes. A cytosolic GAPDH encoding gene （gpd） of Gracilaria/Gracilariopsis lemaneiformis was cloned and characterized. Deduced amino acid sequence of the enzyme of G. lemaneiformis had high homology with those of seven red algae. The 5＇-untranslated regions of the GAPDHs encoding genes of these red algae varied greatly. GAPDHs of these red algae shared the highly conserved glyceraldehyde 3-phosphate dehydrogenase active site ASCTTNCL. However, such active site of Cyanidium caldarium was different from those of the other six algae at the last two residues （CL to LF）, thus the spatial structure of its GAPDH active center may be different from those of the other six. Phylogenetic analysis indicated that GAPDH of G. lemaneiformis might have undergone an evolution similar to those of Porphyra yezoensis, Chondrus crispus, and Gracilaria verrucosa. C. caldarium had a closer evolutionary relationship with Cyanidioschyzon merolae than with Cyanidium sp. Virtual Northern blot analysis revealed that gpd of G. lemaneiformis expressed constitutively, which suggested that it might be house-keeping and could be adapted as an inner control in gene expression analysis of G. lemaneiformis.     

Sensitivity of carbon budget to historical climate variability and atmospheric CO2 concentration in temperate grassland ecosystems in China

Chinese temperate grasslands play an important role in the terrestrial carbon cycle. Based on the parameterization and validation of Terrestrial Ecosystem Model (TEM, Version 5.0), we analyzed the carbon budgets of Chinese temperate grasslands and their responses to historical atmospheric CO₂ concentration and climate variability during 1951–2007. The results indicated that Chinese temperate grassland acted as a slight carbon sink with annual mean value of 7.3 T?g C, ranging from -80.5 to 79.6 T?g C yr^-1. Our sensitivity experiments further revealed that precipitation variability was the primary factor for decreasing carbon storage. CO₂ fertilization may increase the carbon storage (1.4 %) but cannot offset the proportion caused by climate variability (-15.3 %). Impacts of CO₂ concentration, temperature and precipitation variability on Chinese temperate grassland cannot be simply explained by the sum of the individual effects. Interactions among them increased total carbon storage of 56.6 T?g C which 14.2 T?g C was stored in vegetation and 42.4 T?g C was stored in soil. Besides, different grassland types had different responses to climate change and CO₂ concentration. NPP and R_H of the desert and forest steppes were more sensitive to precipitation variability than temperature variability while the typical steppe responded to temperature variability more sensitively than the desert and forest steppes.     

Latest update of the climatology and changes in the seasonal distribution of precipitation over China

Based on daily precipitation data from 524 meteorological stations in China during the period 1960–2009, the climatology and the temporal changes (trends, interannual, and decadal variations) in the proportion of seasonal precipitation to the total annual precipitation were analyzed on both national and regional scales. Results indicated that (1) for the whole country, the climatology in the seasonal distribution of precipitation showed that the proportion accounted for 55 % in summer (June–August), for around 20 % in both spring (March–May) and autumn (September–November), and around 5 % in winter (December–February). But the spatial features were region-dependent. The primary precipitation regime, “summer–autumn–spring–winter”, was located in central and eastern regions which were north of the Huaihe River, in eastern Tibet, and in western Southwest China. The secondary regime, “summer–spring–autumn–winter”, appeared in the regions south of the Huaihe River, except Jiangnan where spring precipitation dominated, and the southeastern Hainan Island where autumn precipitation prevailed. (2) For the temporal changes on the national scale, first, where the trends were concerned, the proportion of winter precipitation showed a significantly increasing trend, while that of the other three seasons did not show any significant trends. Second, for the interannual variation, the variability in summer was the largest among the four seasons and that in winter was the smallest. Then, on the decadal scale, China experienced a sharp decrease only in the proportion of summer precipitation in 2000. (3) For the temporal changes on the regional scale, all the concerned 11 geographic regions of China underwent increasing trends in the proportion of winter precipitation. For spring, it decreased over the regions south of the Yellow River but increased elsewhere. The trend in the proportion of summer precipitation was generally opposite to that of spring. For autumn, it decreased over the other ten regions except Inner Mongolia with no trend. It is noted that the interannual variability of precipitation seasonality is large over North China, Huanghuai, and Jianghuai; its decadal variability is large over the other regions, especially over those regions south of the Yangtze River.     

Identification of Phase and Sex-related ISSR Markers of Red Alga Gracilaria lemaneiformis

Six ISSR primers are employed to display the polymorphism of different phases and sexes of red alga Gracilaria lemaneiformis, and two of them, P1 and P3, amplified distinct band patterns. The ISSR pattern amplified by primer P1 of the female gametophyte is identical to that of tetrasporophyte, but distinct from that of male gametophyte. Of the bands produced by primer P3, one is specific to female gametophyte. Three morphologically similar fronds can be easily identified using ISSR technique. Two specific markers, SM1 and SF3, related to male gametophyte and female gametophyte, are cloned and sequenced. The homologous sequences of SM1 are found to encode a hypothetical protein. There is no homologous sequence of SF3 that can be found in GenBank.     

Magma underplating and Hannuoba present crust-mantle transitional zone composition: Xenolith petrological and geochemical evidence

Studies on the deep-seated xenoliths from global volcanoes reveal that the present petrological crust-mantle boundary between the lower crust and the upper mantle actually is a transitional layer from mainly mafic granulites to ultramafic spinel lher-zolites[1,2], i.e. a transitional zone distinctive from the seismological Moho[3]. Oceanic lithosphere crust- mantle transitional zone can be established from the study on the exposed ophiolites. However, as for the continental lithosphere, since …     

变刚度钢管混凝土短柱隔震结构弹塑性动力分析

采用HBTA2.5程序,对一变刚度钢管混凝土短柱隔震结构进行了弹塑性时程分析。分析结果表明,其隔震装置的水平变形和耗能能力可以大大减轻地震对结构的影响。提出钢管混凝土短柱隔震结构在工程应用将有很好的发展前景。     

气象台站探测环境评估绘图软件设计与实现

文章重点介绍了＂气象台站探测环境评估绘图软件＂项目建设依据和目标、建设思路和原则、建设内容和实现的功能。＂气象台站探测环境评估绘图软件＂是一个集成气象部门地面、高空台站各类探测环境绘图方法,同时具有探测环境评价、评估功能的应用软件。     

非线性编辑系统在天气预报节目中的应用与维护

内蒙古气象影视服务科开发引进非线性编辑系统（以下简称非编系统）制作电视天气预报节目已有几年时间了,在实际工作中各地都积累了一些操作和使用的经验并有所改进和完善。文章根据非编系统的特点,结合平时业务工作中的使用情况,探讨其在天气预报制作中的具体运用和维护。     

A study of the microphysical processes in a numerically simulated heavy snowfall event in North China: the sensitivity of different snow intercept parameters

We performed a modeling study of the cloud processes in a heavy snowfall event occurring in North China on 20–22 December 2004. The nonhydrostatic Mesoscale Model (MM5) was used to carry out experiments with the Reisner-2 explicit microphysical parameterizations in four nested domains to test the sensitivity of simulated heavy snowfall to different snow intercept parameters. Results show that while the different intercept parameters do not significantly affect the accumulated snowfall amounts at the surface in either total amount or location, some microphysical characteristics of the modeled heavy snowfall event are impacted. The budget of cloud microphysics is analyzed to determine the dominant cloud processes. In the control experiment (CTL) with the snow intercept (N _os) specified as a function of temperature, the primary simulated hydrometeor is snow, and its mixing ratio is an order of magnitude larger than that of the other cloud species. Relative to CTL, the experiment with a fixed intercept (CON3E6) produced lower snow mixing ratios, more cloud water and graupel mixing ratios. Among the two experiments, while snowfall is slightly smaller in CON3E6, other processes like the rate of graupel fall, condensation and evaporation of cloud water, deposition and sublimation of graupel are all larger in CON3E6 than in CTL. Among CTL, CON3E6, and two more experiments (CON2E7: with a smaller fixed intercept; and NOSQS: N _os a function of snow mass mixing ratio), the budget shows that CON3E6 produces the smallest deposition and sublimation of snow, the largest deposition of cloud ice, and the largest conversion from cloud ice to snow.     

2008年11月黑龙江省3次较大降雪过程云图对比分析

本文针对2008年11月2日、13日和15日3次较大降雪天气过程,从卫星云图应用等方面分析了3种不同的云系在降雪过程中的演变情况,并结合700 hPa高空网进行分析.初步得出3种不同云系出现大降雪的一些特征.