Hydrothermal Fluid Sources of the Fengjia Barite–fluorite Deposit in Southeast Sichuan,China: Evidence from Fluid Inclusions and Hydrogen and Oxygen Isotopes

The Fengjia barite–fluorite deposit in southeast Sichuan is a stratabound ore deposit which occurs mainly in Lower Ordovician carbonate rocks. Here we present results from fluid inclusion and oxygen and hydrogen isotope studies to determine the nature and origin of the hydrothermal fluids that generated the deposit. The temperature of the ore‐forming fluid shows a range of 86 to 302 °C. Our detailed microthermometric data show that the temperature during mineralization of the fluorite and barite in the early ore‐forming stage was higher than that during the formation of the calcite in the late ore‐forming stage. The salinity varied substantially from 0.18% to 21.19% NaCl eqv., whereas the density was around 1.00 g/cm³. The fluid composition was mainly H₂O (>91.33%), followed by CO₂, CH₄ and traces of C₂H₆, CO, Ar, and H₂S. The dominant cation was Na⁺ and the dominant anion Cl^‐, followed by Ca²⁺, SO₄^2‐, K⁺, and Mg²⁺, indicating a mid–low‐temperature, mid‐low‐salinity, low‐density NaCl–H₂O system. Our results demonstrate that the temperature decreased during the ore‐forming process and the fluid system changed from a closed reducing environment to an open oxidizing environment. The hydrogen and oxygen isotope data demonstrate that the hydrothermal fluids in the study area had multiple sources, primarily formation water, as well as meteoric water and metamorphic water. Combined with the geological setting and mineralization features we infer that the stratabound barite–fluorite deposits originated from mid–low‐temperature hydrothermal fluids and formed vein filling in the fault zone.     

Curvedness feature constrained map matching for low-frequency probe vehicle data

Map matching method is a fundamental preprocessing technique for massive probe vehicle data. Various transportation applications need map matching methods to provide highly accurate and stable results. However, most current map matching approaches employ elementary geometric or topological measures, which may not be sufficient to encode the characteristic of realistic driving paths, leading to inefficiency and inaccuracy, especially in complex road networks. To address these issues, this article presents a novel map matching method, based on the measure of curvedness of Global Positioning System (GPS) trajectories. The curvature integral, which measures the curvedness feature of GPS trajectories, is considered to be one of the major matching characteristics that constrain pairwise matching between the two adjacent GPS track points. In this article, we propose the definition of the curvature integral in the context of map matching, and develop a novel accurate map matching algorithm based on the curvedness feature. Using real-world probe vehicles data, we show that the curvedness feature (CURF) constrained map matching method outperforms two classical methods for accuracy and stability under complicated road environments.     

Advanced glycation endproducts in 35 types of seafood products consumed in eastern China

Advanced glycation endproducts(AGEs) have been recognized as hazards in processed foods that can induce chronic diseases such as cardiovascular disease, diabetes, and diabetic nephropathy. In this study, we investigated the AGEs contents of 35 types of industrial seafood products that are consumed frequently in eastern China. Total fluorescent AGEs level and Nε-carboxymethyl-lysine(CML) content were evaluated by fluorescence spectrophotometry and gas chromatography-mass spectrometry(GC-MS), respectively. The level of total fluorescent AGEs in seafood samples ranged from 39.37 to 1178.3 AU, and was higher in canned and packaged instant aquatic products that were processed at high temperatures. The CML content in seafood samples ranged from 44.8 to 439.1 mg per kg dried sample, and was higher in roasted seafood samples. The total fluorescent AGEs and CML content increased when seafood underwent high-temperature processing, but did not show an obvious correlation. The present study suggested that commonly consumed seafood contains different levels of AGEs, and the seafood processed at high temperatures always displays a high level of either AGEs or CML.     

Phase characteristic analysis of continuous depth air-gun source wavelet

Air guns are important sources for marine seismic exploration. Far-field wavelet of air gun arrays, as a necessary parameter for pre-stack processing and source models, plays an important role during marine seismic data processing and interpretation. When an air gun fires, it generates a series of air bubbles. Similar to onshore seismic exploration, the water forms a plastic fluid near the bubble; the farther the air gun is located from the measurement, the more steady and more accurately represented the wavelet will be. In practice, hydrophones should be placed more than 100 m from the air gun; however, traditional seismic cables cannot meet this requirement. On the other hand, vertical cables provide a viable solution to this problem. This study uses a vertical cable to receive wavelets from 38 air guns and data are collected offshore Southeast Qiong, where the water depth is over 1000 m. In this study, the wavelets measured using this technique coincide very well with the simulated wavelets and can therefore represent the real shape of the wavelets. This experiment fills a technology gap in China.     

Establishment and characterization of a testicular Sertoli cell line from olive flounder <Emphasis Type="Italic">Paralichthys olivaceus</Emphasis>

The culture of Sertoli cells has become an indispensable resource in studying spermatogenesis. A new Sertoli cell line (POSC) that consisted predominantly of fibroblast-like cells was derived from the testis of the olive flounder Paralichthys olivaceus and sub-cultured for 48 passages. Analysis of the mtDNA COI gene partial sequence confirmed that the cell line was from P. olivaceus. Cells were optimally maintained at 25°C in DMEM/F12 medium supplemented with fetal bovine serum, basic fibroblast growth factor, and epidermal growth factor. The growth curve of POSC showed a typical “S” shape. Chromosome analysis revealed that the cell line possessed the normal P. olivaceus diploid karyotype of 2n=48t. POSC expressed dmrt1 but not vasa, which was detected using RT-PCR and sequencing. Immunocytochemistry revealed that the cells exhibited the testicular Sertoli cell marker FasL. Therefore, POSC appeared to consist of testicular Sertoli cells. Bright fluorescent signals were observed after the cells were transfected with pEGFP-N3 plasmid, with the transfection efficiency reaching 10%. This research not only offers an ideal model for further gene expression and regulation studies on P. olivaceus, but also serves as valuable material in studying fish spermatogenesis, Sertoli cell-germ cell interactions, and the mechanism of growth and development of testis.     

An improved ionosphere interpolation algorithm for network RTK in low-latitude regions

A traditional interpolation algorithm with the linear interpolation method (LIM) using a fixed number of reference stations is widely used in network RTK to obtain the ionospheric delays for the users. In low-latitude regions, where the ionosphere is relatively active, however, large interpolation errors exist, especially for satellites at low elevation angles. Considering the characteristics of “coinciding ionospheric pierce points (CIPPs)” with a similar nature of ionospheric delays, an improved interpolation algorithm is proposed. In this algorithm, all stations with CIPPs are used to establish the interpolation model; thus, more precise interpolation model is achieved. To validate the performance of the proposed algorithm, data from some reference stations in Guangdong Province of China were used, and the results are compared with those with the traditional interpolation algorithm. Numerical analysis shows that the interpolation accuracy of the proposed algorithm increases by 10–30% compared with the traditional one. Since the number of reference stations is flexible, the proposed algorithm can also balance the model accuracy with the computation burdens. In addition, the proposed algorithm is less affected by the selection of master reference station. In terms of network RTK on-the-fly positioning, the time-to-first-fix is reduced when replacing the traditional interpolation algorithm with the proposed one.