固定化鲅鱼乙酰胆碱酯酶的制备及部分性质测定

酶的固定化是酶传感器制备过程中重要的1个环节.研究采用直接共价法固定鲅鱼乙酰胆碱酯酶（AChE）.制备方法为：0.1g CNBr活化的Sepharose 4B凝胶用1mmol/L的HCl充分溶胀后,与活力为10U的AChE溶液混合,于4℃下150r/min振荡8h.所制备的固定化酶活力回收率较高（96%）,对pH值和温度变化的适应能力均优于非固定化酶;在3个月保存期内,前者的活力损失13%,而后者的活力则下降89%.这说明固定过程能够大大提高AChE的抗逆性和保持酶活力的稳定,有利于酶传感器的制备.     

FINITE ELEMENT METHOD IN CALCULATION OF KUROSHIO CURRENT

We establish the equations of finite element approximation for three-dimensional ocean current and calculate the Kuroshio current in the eastern sea area off Taiwan Province. The interpolation error of finite element approximation is given as Eq. (3. 14).     

筒型基础沉贯阻力估算方法

系统地总结国外有代表性工程实践对筒型基础沉贯阻力的研究 ,并将各种方法综合起来 ,提出了加权综合估算法 ,供今后的研究和工程实践参考     

西太平洋暖池变异及其对西太平洋次表层海温场的影响

应用热带太平洋上层XBT温度资料,分析研究了西太平洋暖池区(0°～16°N,125°～145°E)上层海洋的变化特征以及与西太平洋次表层海温场之间的关系.研究表明,西太平洋暖池区的垂向温度存在显著的年际变化,尤其在次表层(120～200m)的变化最为明显.西太平洋暖池区的次表层冷暖信号明显早于西太平洋次表层的海温异常.分析发现,西太平洋暖池区的海温异常是导致整个西太平洋次表层海温场变异的关键区,当西太平洋暖池区的次表层冷暖信号加强时,3～4个月后西太平洋海温场出现大范围的冷暖异常.     

富钴结壳分布的分形特征

利用我国在西太平洋国际海底两个海山获得的富钴结壳资料进行分形研究后发现，富钴结壳的分布具有变维分形分布的特点，结壳丰度呈二阶累计和分形分布，结壳厚度呈多阶不定累计和分形分布，通过计算得到了它们各自的分维数，进而对两个海山的结壳资源进行了初步评价。     

南海上层海洋热含量的年际和年代际变化

分析了1959—1988年南海表面至100m垂直平均温度（TAV）资料，结果表明：南海上层海洋热含量存在明显的准两年、4—5年和年代际振动。在E1Nino年，南海上层热含量显著增加。50年代末至70年代初，南海TAV为负距平，此后转为正距平。南海TAV的变化与ENSO事件、东亚冬季风和热带大气环流的变异密切相关。     

The influence of 30~60 d oscillation on the development of the South China Sea summer monsoon

Using the National Center for Enviromental Prediction/National Center for Atmospheric Research (NCEP/NCAR) reanalysis data and NOAA satellite-obser ved outgoing long-wave radiation (OLR) data, the development of the South China Sea (SCS) summer monsoon and intraseasonal (30-60 d) oscillation (ISO) have been examined. The results show that there exists obvious interannual variability of intraseasonal oscillaiton. Using the 16 a time series of filtered OLR averaged over the SCS, an index is defined to define ““onset events““ over the SCS on the ISO time scales. Of the 16 a examined here, 10 shows a strong ISO signal in the onset of monsoon convection over the SCS. In these cases, the ISO initially suppresses the seasonal development of southwesterly and cyclonic circulation over the SCS before the ISO onset. As the ISO propagates northeastward, the low frequency cyclonic circulation anomaly occurs in the SCS and the low frequency southwesterly wind and convection over here dramatically intensify. The northeast progression of the ISO anomaleis plays a role in the initial suppression and then acceleration of the seasonal cycle of the SCS summer monsoon.     

纳米碳酸钙材料的工业合成与应用

该文对目前工业上生产纳米碳酸钙的方法作了较为详细的综述。对一步碳化法、两步碳化法、多段喷雾碳化法、旋转填充床碳化反应器碳化技术制备链形、纺锤形、球形、立方形等不同形状的纳米碳酸钙材料的方法进行了比较和总结     

Effects of chemical ecological adjustment and control experiment on phytoplankton community in the Aoshan Bay

There is a low nutrient level in the Aoshan Bay. In June 1999, the chemical adjustmentand control experiment was made in the Aoshan Bay. Following tracts investigation was carried out before the experiment and on the 1st, 2nd, 4th, 5th, 6th and 45th day/after the experiment. While the variance of amount of phytoplankton, the replacement of superior species and the species composition of phytoplankton were researched. The results show that the amount of phytoplankton in the Aoshan Bay rises gradually after the experiment. Ceratium macroceros Cleve of pyrophyta was the dominant species before the experiment, its dominant index was 37.7％. Six days after the experiment, its dominant index dropped to 17.6％ . Meanwhile the dominant index of Asterionella japanics Cleve rose from 7.1％ to 39.2％ , it became the first dominant species. Forty-five days after the experiment, the amount of phytoplankton in the Aoshan Bay was 5.15 to 137.32 times more than that in 1997.     

Development of a real-time PCR method for Thalassiosira rotula rapid detection

Gene specific primers and DNA probe were designed based on the sequence of 18S rDNA cloned from the red tide alga Thalassiosira rotula. A real-time fluorescent quantitative PCR (RFQ-PCR) method was developed for quantitative detection of T.rotula. The RFQ-PCR assay data showed that the results obtained with the RFQ-PCR quite good agreement with those with the light microscope (LM) counting method, which suggested that the RFQ-PCR could be a useful method for red tide alga detection.