Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.     

载波/多普勒平滑伪距在Android手机中的定位实现

针对Android手机GNSS伪距定位精度较低的问题，利用手机端观测信息，通过载波相位/多普勒平滑伪距改善手机端伪距观测值的质量，从而达到提高定位精度的效果。首先给出了Android手机GNSS原始观测量的获取方法，然后推导了载波相位平滑伪距和多普勒平滑伪距算法模型，并设计合理有效的试验对算法的精度进行评定。试验结果表明：在手机端静态定位中载波相位和多普勒平滑算法均可提高原始伪距的定位精度，且多普勒平滑算法表现更优；在手机端动态定位中多普勒平滑算法可获得比原始伪距更优的定位精度，但是载波相位平滑算法较原始伪距更差；由于硬件的制约手机端周跳和信号失锁严重，占比超过50%，载波相位在手机端的可用性较低；多普勒平滑算法的最优平滑时间常数小于等于10 s，具有实时动态定位的巨大潜力。     

Effects of stocking density on the growth and immunity of Atlantic salmon salmo salar reared in recirculating aquaculture system (RAS)

Atlantic salmon reared in recirculating aquaculture system (RAS) may lead to inappropriately high stocking density, because fish live in a limited space. Finding the suitable stocking density of Atlantic salmon reared in RAS is very important for RAS industry. In this paper, the influence of stocking density on growth and some stress related physiological factors were investigated to evaluate the effects of stocking density. The fish were reared for 220 days at five densities (A: 24 kg/m³; B: 21 kg/m³; C: 15 kg/m³; D: 9 kg/ m³ and E: 6 kg/m³ ). The results show that 30 kg/m³ might be the maximum density which RAS can afford in China. The stocking densities under 30 kg/m³ have no effect on mortality of Atlantic salmon reared in RAS. However, the specific growth rate (SGR), final weight and weight gain in the high density group were significantly lower than the lower density groups and middle density groups. Moreover, feed conversion rate (FCR) had a negative correlation with density. Plasma hormone T₃ and GH showed significant decrease with the increase of the stocking density of the experiment. Furthermore, thyroid hormone (T₃), GH (growth hormone) activities were decreased with stocking density increase. However, plasma cortisol, GOT (glutamic oxalacetic transaminase) and GPT (glutamic pyruvic transaminase) activities were increase with stocking density increase. And the stocking density has no effects on plasma lysozyme of Atlantic salmon reared in RAS. These investigations would also help devise efficient ways to rear adult Atlantic salmon in China and may, in a way, help spread salmon mariculture in China.

     

基于深度域地震成像的中沙海槽盆地东北部结构构造研究

文章基于叠前深度偏移地震成像分析, 结合前人重磁反演等地球物理资料成果, 对中沙海槽盆地东北部结构构造进行探索研究。研究表明: 中沙海槽盆地东北部发育新生代地层, 厚度在1500~2500m之间, 地层层速度为1500~ 3500m·s^-1, 不存在中生界沉积层, 盆地基底为海山或前寒武系基岩及岩浆岩隆起。中沙海槽盆地新生代陆源海相烃源岩丰富, 盆地新生界地层厚度大, 盆地凹陷、断裂发育, 具有一定的油气勘探潜力。     

自然和自然贡献情景模型研究综述

本文回顾了自然和自然贡献情景模型发展的背景、历史和内容,概括总结了自然和自然贡献情景模型的发展进程以及联合国生物多样性与生态系统服务政府间科学—政策平台(IPBES)情景模型的概念框架,讨论了自然和自然贡献情景模型存在的问题和发展方向。为了在全球层面解决现有综合集成模型存在的问题,根据地球表层建模基本定理和生态环境曲面建模基本定理,提出了具有中国原创特点的自然与自然贡献情景模型概念框架。     

基于细分行业的安徽省创新型企业集聚研究

创新型企业作为塑造创新地理格局的重要力量，其区位选择的外部特征及内在机理亟须予以系统化研究。基于对安徽省创新型企业的细分行业与空间建库，采用核密度估计和地理探测器等方法，对细分行业下的创新型企业空间集聚特征及影响因素进行研究。结果表明：（1）安徽省创新型企业类型以先进制造和自动化为主，并以生物与新医药、新材料、电子信息为辅；（2）创新型企业空间集聚的不均衡特征显著，呈现出“一核一带多节点”的集聚分布特征，且合肥、芜湖是两个重要集聚区；不同细分行业视角下创新型企业的空间分布形态各异，可归纳为点-轴式、极核式、双核式及多核分散式等4种空间组织形态。（3）创新型因子对于创新型企业的空间集聚最为重要，但传统型和政策型因子的重要性仍不容忽视。其中，产业基础、人力资本、创新投入是最核心的因素，但各行业创新型企业的影响因素作用力则表现出一定的异质性。     

基于 SRP 概念模型的祁连山地区生态脆弱性评价

基于生态敏感性-生态恢复力-生态压力度（SRP）概念模型，从地形、气候、植被和社会经济 因子选取 8 个评价指标，利用遥感和 GIS 技术，采用主成分分析方法求取权重，对祁连山地区启动 水源涵养区生态环境保护和综合治理规划研究前后近 10 a 的生态脆弱性程度进行系统、定量地评 估，旨在揭示生态脆弱性的分布特征、时空演变及动因，为区域生态保护、资源利用和可持续发展 提供参考。结果表明：（1）从研究区生态脆弱性分布来看，祁连山地区主要以轻度和重度脆弱为 主，脆弱性程度从西北向东南地区逐渐减弱，西北地区植被覆盖度小，海拔高，生态环境较为恶劣 是导致脆弱性程度较高的原因；（2）祁连山地区 3 期生态脆弱程度呈逐渐下降趋势，综合指数分别 为 3.307、3.118 和 3.103；2005 年 生 态 脆 弱 性 较 高 ，极 度 脆 弱 面 积 为 28 610 km²，2010 年 下 降 为 11 723 km²，2015 年降低为 6 174 km²，极度脆弱面积逐渐减少；（3）从祁连山地区生态脆弱性演变动 因来看，8 个指标对生态脆弱性影响均较为显著，但在不同的时间影响程度各不相同，2005—2015 年 3 期数据中对生态脆弱性影响最大的均为植被指数，降水次之，地形因子影响最小。总体来看， 近年来祁连山地区生态脆弱性程度有所降低，但仍然需要加强保护力度，促进生态环境可持续 发展。     

广西田阳-那坡县堆积铝土矿分布规律初探

文章试图通过古地理环境、地质构造条件、沉积铝土矿及矿区地貌等因素去初步探讨田阳县-那坡县一带堆积铝土矿优质矿石及含矿率分布的规律性.认为沉积铝土矿及地貌因素是控制该地区堆积铝土矿优质矿石及含矿率分布的主要因素.     

A STUDY ON LAND SYSTEM INNOVATIONS IN THE CONSTRUCTION OF SMALL TOWNS IN CHINA

The economic development in China and the rising of the living standard need to speed up the urbanisa-tion.The development of small towns is an important way to Chinese urbanization.Land use plays a very important role in the development of small towns.However there are many problems in the development of small towns,esp.in land use.The paper first discusses the land problems in the development of small towns.Such as much cultivated land lies idle,under-utilization and waste of land,increasing illegal use of land,unstable contractural relationship for land use.The relationship between the development of small towns and land use is also studied.Then the guidelines for the land system innovations of small towns are put forward.Namely the sustainability of social and economic development,the bal-ance between land reservation and land utilization,the provision of service to village ,agriculture and farmer,the manage-ment of land resource and land assets,the parsimonious andlegal use of land.The basic framework of land system innova-tions of small towns is put forward finally.It include the land replacement policy for small towns and the permanent tenan-cy of farmland,the overall plan for land uses and other plans in harmony,the establishment of a flexible system of land supplies,using land with payments ,the transfer of agricultural land and refining the land law related to the construction of small towns.     

RTK技术在物探测量应用中若干问题探讨

GPS实时动态定位（RTK）技术在测绘领域得到了广泛应用。在分析RTK工作原理及其优缺点的基础上，对在施工中出现的问题进行了分类和归纳，提出了解决方案，并对RTK技术发展方向进行了探讨，目的是提高RTK测量成果精度，使RTK技术更好的推广应用，满足三维和高分辨率地震勘探技术要求，提高作业速度，减轻测量人员劳动强度。