Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.     

烟台市地质灾害发育现状及影响因素分析

2016年山东省开展各市地质灾害排查工作,烟台市作为地质灾害较发育区,是该次排查重点工作区之一。该文以现状调查为基础,查明了烟台市共发育471处地质灾害隐患点,地质灾害规模以小型为主,主要类型为崩塌、滑坡、泥石流、采空塌陷和岩溶塌陷,其中崩塌隐患点占地质灾害总数的68%,数量最多,其次为泥石流和采空塌陷,各占总数的15%和14%,滑坡数量最少,仅占总数的1%,并介绍了各类地质灾害分布特征。在此基础上,对地质灾害的主要影响因素——地形地貌、岩土体类型、地质构造、降水和人类工程活动等进行了分析。最后提出了在进行工程建设活动的同时重视其引发的地质灾害问题,以减少和预防其带来的次生地质灾害。     

秦皇岛近岸海域绿藻微观繁殖体的分布以及在绿潮形成中的作用

2015年以来，秦皇岛近岸海域暴发了绿潮，对北戴河旅游区的环境和生态系统造成了严重影响。绿藻微观繁殖体在绿潮的形成过程中起到重要作用，主要包括孢子、配子、幼苗和营养片段。绿藻微观繁殖体作为绿潮的“种源”，其分布规律可以反映绿潮的“藻源”位置。本研究于2016年4-9月和2017年1月对秦皇岛近岸海域绿藻微观繁殖体的调查，探究了其分布规律以及生物量变化。结果显示，绿藻微观繁殖体主要分布在近岸海域，由近岸向远岸海域逐渐降低。绿藻微观繁殖体的数量在7、8月份最高，在冬季最低。受绿潮影响严重的海域微观繁殖体数量高于其它海域。秦皇岛近岸海域的绿藻微观繁殖体为该海域绿潮的种源，其分布规律表明秦皇岛近岸海域绿潮起源于本地。     

A machine learning approach for predicting computational intensity and domain decomposition in parallel geoprocessing

ABSTRACT

High performance computing is required for fast geoprocessing of geospatial big data. Using spatial domains to represent computational intensity (CIT) and domain decomposition for parallelism are prominent strategies when designing parallel geoprocessing applications. Traditional domain decomposition is limited in evaluating the computational intensity, which often results in load imbalance and poor parallel performance. From the data science perspective, machine learning from Artificial Intelligence (AI) shows promise for better CIT evaluation. This paper proposes a machine learning approach for predicting computational intensity, followed by an optimized domain decomposition, which divides the spatial domain into balanced subdivisions based on the predicted CIT to achieve better parallel performance. The approach provides a reference framework on how various machine learning methods including feature selection and model training can be used in predicting computational intensity and optimizing parallel geoprocessing against different cases. Some comparative experiments between the approach and traditional methods were performed using the two cases, DEM generation from point clouds and spatial intersection on vector data. The results not only demonstrate the advantage of the approach, but also provide hints on how traditional GIS computation can be improved by the AI machine learning.     

基于细分行业的安徽省创新型企业集聚研究

创新型企业作为塑造创新地理格局的重要力量，其区位选择的外部特征及内在机理亟须予以系统化研究。基于对安徽省创新型企业的细分行业与空间建库，采用核密度估计和地理探测器等方法，对细分行业下的创新型企业空间集聚特征及影响因素进行研究。结果表明：（1）安徽省创新型企业类型以先进制造和自动化为主，并以生物与新医药、新材料、电子信息为辅；（2）创新型企业空间集聚的不均衡特征显著，呈现出“一核一带多节点”的集聚分布特征，且合肥、芜湖是两个重要集聚区；不同细分行业视角下创新型企业的空间分布形态各异，可归纳为点-轴式、极核式、双核式及多核分散式等4种空间组织形态。（3）创新型因子对于创新型企业的空间集聚最为重要，但传统型和政策型因子的重要性仍不容忽视。其中，产业基础、人力资本、创新投入是最核心的因素，但各行业创新型企业的影响因素作用力则表现出一定的异质性。     

Fluid Inclusion Feature of Gold-Bearing Quartz Veins from the Jinniushan Gold Deposit, Shandong, China

The auriferous veins at Jinniushan occurs within the Jinniushan faulted zone in the Kunyushan Granite. Optical observation reveals that gold ore body formed during the main stage of hydrothermal activity. Detailed geothermometric studies of fluid inclusions from the veins show that the forming temperature ranges between 130℃ and 370℃ and the salinity is from 4.01 to 15.21 wvt percent NaCl. The ore-forming fluid is featured by low to moderate salinity, and low to moderate temperature. According to investigations of the values of vapor/liquid and temperatures of the ore-forming fluids, we propose that the boiling fluid inclusions exist in the main mineralization stages. Fluid boiling is suggested as a mechanism for the precipitation of gold from the hydrothermal fluid in the Jinniushan gold deposit.     

Characteristics of Spatial and Temporal Variations of Monthly Mean Surface Air Temperature over Qinghai-Tibet Plateau

1 Introduction The Qinghai-Tibet Plateau, known as the highest plateau with the most complex topography in the world, covers an area of more than 200km2, with a mean elevation of more than 4000m a.s.l. (Ye and Gao, 1979). Surrounded by the Earth’s highest mountains, such as the Himalayas, Pamir, Kunlun Mountains, the plateau plays a significant role in climate change in China even in the world, thus attracted great attention of researchers. Up to now, many achievements have been gained by…     

Observational evidence for solar coronal decimetric radio pulsations with very short periods

Using the decimetric (700–1500 MHz) radio spectrometer and the synchronous observational system with high temporal resolution at four frequencies (1420, 2130, 2840 and 4260 MHz) of Yunnan Observatory, two rare events were observed on 2001 June 24 and 1990 July 30. The former was a small radio burst exhibiting pulsations with short periods (about 29, 40 and 100 ms) in the impulsive phase. The latter was a large radio burst, which at 2840 MHz produced radio pulsations with period of about 30 ms. This paper focuses on pulsations with very short periods in the range of 29–40 ms. The mechanism of generation of such pulsations may be modulation of radio radiation by the periodic trains of whistler packets originating in unstable regions of the corona. Alternatively, these pulsations can be attributed to wave-wave non-linear interactions of electrostatic upper hybrid waves driven by beams of precipitating electrons in flaring loops.     

AR（P）参数估计协方差方法与其改进型方法的对比研究

定性分析协方差方法及其改进型在ＡＲ（Ｐ）参数估计中的应用，提供了一种估计参数的计算机上机流程图，比较了协方差方法及其改进型谱估计结果。     

对地方电视台转播CCTV时延的测定

讲述了同步广播卫星电视时间信号的测量方法和测量结果，获得了CCTV1、CCTV2通过同一颗卫星（亚太－1A）转播的时延差值为16333μs，测量精度在10ns以内；CCTV2、CCTV4分别经两颗卫星（亚太－1A、亚洲－Ⅱ）转播，在陕西天文台卫星地面接收站时延差值为1644．20μs，精度为50μs，并分析了影响时延差值和精度的原因。同时测量了地方电视台转播亚太－1A的CCTV信号与直接接收亚太－1A的CCTV信号的时延差值，其测量精度为0．1μs。这些结果为利用同步广播卫星的电视信号进行高精度的时间服务提供了参考依据。