Enhanced a novel β-agarase production in recombinant Escherichia coli BL21(DE3) through induction mode optimization and glycerol feeding strategy

Agarases are hydrolytic enzymes that act on the hydrolysis of agar and have a broad range of applications in food,cosmetics and pharmaceutical industries. In this study, a glycerol feeding strategy based on induction mode optimization for high cell density and β-agarase production was established, which could effectively control acetate yield. First, exponential feeding strategy of glycerol with different overall specific growth rates(μ) was applied in the pre-induction phase. The results showed that the low μ(μ=0.2) was suggested to be the optimal for cell growth and β-agarase production. Second, the effects of induction temperature and the inducer concentration on cell growth and β-agarase production were investigated in the post-induction phase. When induced by isopropyl-β-d-thiogalactoside(IPTG), the strategy of 0.8 mmol/L IPTG induction at 20℃ was found to be optimal for β-agarase production. When cultivation was induced by continuous lactose feeding strategy of 1.0 g/(L·h), the β-agarase activity reached 112.5 U/mL, which represented the highest β-agarase production to date.Furthermore, the β-agarase was capable of degrading G. lemaneiformis powder directly to produce neoagarooligosaccharide, and the hydrolysates were neoagarotetraose(NA4) and neoagarohexaose(NA6). The overall research may be useful for the industrial production and application of β-agarase.     

浙南近海虾类群落结构及其多样性分析

作者根据2015年11月(秋季)、2016年2月(冬季)、2016年5月(春季)和2016年8月(夏季)对浙南近海进行的渔业资源调查数据,用生物量作为虾类资源分布的数量指标,对该海域虾类的组成、数量分布以及季节变化进行分析。结果显示:该区域共鉴定出虾类30种,隶属于10科21属,以对虾科(Penaeidae)虾类最多,其次为管鞭虾科(Solenoceridae),长臂虾科(Palaemonida)位居第3位。按季节来看,秋季共鉴定虾类27种,各站位平均生物量为6.97 kg/km2,优势种为凹陷管鞭虾(Solenocera koelbeli)、中国毛虾(Acetes chinensis)和中华管鞭虾(Solenocera crassicornis)等3种;冬季鉴定出虾类25种,平均生物量为1.65 kg/km2,优势种为凹陷管鞭虾、扁足异对虾(Atypopenaeus stenodactylus)、日本鼓虾(Alpheus japonicus)、鲜明鼓虾(Alpheus digitalis)、中国毛虾、中华管鞭虾和周氏新对虾(Metapenaeus joyneri)等7种;春季20种,平均生物量为2.22 kg/km2,优势种为戴氏赤虾(Metapenaeopsis dalei)、东海红虾(Metapenaeopsis dalei)和中华管鞭虾等3种;夏季16种,平均生物量为10.36 kg/km2,优势种有鹰爪虾(Trachysalambria curvirostris)和中华管鞭虾等2种。Margalef丰富度指数(D)、Shannon-Wiener多样性指数(H′)和Pielou均匀度指数(J′)秋季均低于冬季。秋冬季受到浙闽沿岸流影响,温度、盐度相对较低,夏季受到台湾暖流外侧影响,温度、盐度相对较高,且各季节间由于瓯江、椒江、飞云江、鳌江等陆源性冲淡水的影响,盐度、温度变化相对较大,使得该区域的物种不得不适应一个跨度较大的温盐范围,广温广盐性种类为主。     

A terrain openness index for the extraction of karst Fenglin and Fengcong landform units from DEMs

The Fenglin and Fengcong landform units are considered to be an important representation for defining the degree of development of Karst landforms. However, these terrain features have been proven difficult to delineate and extract automatically because of their complex morphology. In this paper, a new method for identifying the Fenglin and Fengcong landform units is proposed. This method consists of two steps: (1) terrain openness calculation and (2) toe line extraction. The proposed method is applied and validated in the Karst case area of Guilin by using ASTER GDEM with one arc-second resolution. The openness of both the positive and negative terrain and a threshold were used to extract toe lines for segmenting depressions and pinnacles in Fenglin and Fengcong landforms. A comparison between the extracted Fenglin and Fengcong landform units and their real units from high resolution images was carried out to evaluate the capability of the proposed method. Results show the proposed method can effectively extract the Fenglin and Fengcong landform units, and has an overall accuracy of 93.28%. The proposed method is simple and easy to implement and is expected to play an important role in the automatic extraction of similar landform units in the Karst area.     

基于噪声谱比法的内蒙古西部地区台站场地响应研究

利用内蒙古西部12个台站的地脉动噪声数据，采用噪声谱比法研究台站的场地响应情况。分析表明，台站场地响应按曲线形态可分为3类，且可能受地形地貌、局部构造和台基状况等条件影响。对比分析噪声谱比法与Moya方法的场地响应结果发现，场地响应曲线形态基本一致，只有极少数台站存在明显差异。结果表明，内蒙古西部区域大部分台站的台基状况总体较好，场地响应曲线较为平坦，无明显频率放大点。     

Prediction of Blast-Induced Rock Movement During Bench Blasting: Use of Gray Wolf Optimizer and Support Vector Regression

Natural Resources Research - A large ore loss and dilution can be expected when using a pre-blast ore boundary for shovel guidance because of the movement and re-distribution of ore in the muck...     

Analysis of Spatial–Temporal Evolution of Mining-Induced Fracture Field: A Case Study Using Image Processing in the Shaqu Coal Mine,China

Natural Resources Research - Mining-induced fracture plays a key role in gas drainage for gas burst-prone underground coal mines, especially for closely multilayered coal seams. The layout and...     

Fluid Inclusion Feature of Gold-Bearing Quartz Veins from the Jinniushan Gold Deposit, Shandong, China

The auriferous veins at Jinniushan occurs within the Jinniushan faulted zone in the Kunyushan Granite. Optical observation reveals that gold ore body formed during the main stage of hydrothermal activity. Detailed geothermometric studies of fluid inclusions from the veins show that the forming temperature ranges between 130℃ and 370℃ and the salinity is from 4.01 to 15.21 wvt percent NaCl. The ore-forming fluid is featured by low to moderate salinity, and low to moderate temperature. According to investigations of the values of vapor/liquid and temperatures of the ore-forming fluids, we propose that the boiling fluid inclusions exist in the main mineralization stages. Fluid boiling is suggested as a mechanism for the precipitation of gold from the hydrothermal fluid in the Jinniushan gold deposit.     

重庆地面最高气温与最大风速年极值的渐近分布

利用重庆1951-1990年间地面最高气温和1956-1990年间地面最大风速年极值的记录,采用韦伯分布和耿贝尔分布分别对其渐近分布进行拟合.通过统计推断,找出重庆地面最高气温和地面最大风速年极值遵循的最佳渐近分布--韦伯分布.     

Optimal Filtering Algorithm for Stochastic 2-D FMM Ⅱ with Multiplicative Noise

1　Introduction　Inrecentyearstherehasbeengrowingresearchin terestinthetwo dimensional (2 D )systemtheory .The 2 Dsystemsmayfindapplicationsinareassuchasmarineseismicdataprocessingandimageprocessing .Althoughmoreandmorevaluableresultshavebeengained ,mos…     

Does Einstein's G.R. theory predict gravitational radiation of PSR 1913+16?

A critical re-examination of the self-consistency of Einstein's gravitational radiation theory is given in the present paper with special reference to its claimed prediction of the observed data on PSR 1913+16.The author is Alfred Yu