合浦珠母贝26S蛋白酶体S4,S7亚基基因片段的分离与分析

26S蛋白酶体是真核生物中一种具有ATP依赖性的蛋白酶复合体,主要通过泛肽途径选择性降解细胞内与代谢调控、细胞周期有关的功能蛋白及异常蛋白,参与多种细胞活动的调控过程。26S蛋白酶体由具有催化活性的20S亚复合体和一个具有调节作用的19S亚复合体组成,其中19S亚复合体中的ATP酶亚基是调节26S蛋白酶体活性的重要组件。本通过简并引物PCR手段,从软体动物合浦珠母贝(Pinctada fucata)中扩增到参与构成19S亚复合体的S4和S7(MSS1)两个亚基的基因片段。这两个基因片段所编码的ATP酶组件包含有Gx4GKT,DEID,SAT和H／QRxGRxxR等26S蛋白酶体ATP酶亚基的共同功能基序。这是首次在软体动物中报道26S蛋白酶体的ATP酶亚基基因序列,为研究软体动物中26S蛋白酶体的结构与功能奠定了分子基础。     

太湖表面定振波的数值计算和最大熵谱分析

利用水动力学方程对太湖表面的定振波进行计算，算得定振波周期约为４５２ｍｉｎ，另外，利用１９９２年８月２９－３１日在太湖西山观测到的水位资料，采用最大熵谱法，分析太湖表面的定振波，得周期值约为４５０ｍｉｎ。计算和分析的周期值基本吻合，取熵谱分析结果得太湖表面的单节点定振波周期值为４５０ｍｉｎ。     

机载雷达高度计对海况及海面风速的测量研究

运用我国自行研制的ＺＨＧ－１型Ｘ波段机载雷达高度计于１９９５年４月１３日－４月１８日在青岛附近海域进行的海上飞行实验数据，及导出的均方根波高、有效波高、均方根波陡算法模型，得到了上述海况参数值。     

Nonlinear tidal waves in a kind of estuary with gradually varying cross-section

-Nonlinear tidal waves in a kind of estuary are studied in the paper using one-dimensional nonlinear hydrody-namic equations with friction. The estuary has exponentially varying width B=B0 e-bx and uniform depth h. The one-dimensional hydrodynamic equations are solved by perturbation method. It was found that our solution included two special cases, Pelisenpeki's solution and Airy's solution. The former can be got by letting b=0 in our solutions, and the latter by setting 6 = 0 and f= 0 (f is linear frictional coefficient). In terms of the second-order solution, the physical mechanism of nonlinear tidal waves in estuaries with gradually varying cross-section is explored. It is shown that, under the assumption of linear friction coefficient, shallow water constituent waves consist of two parts, one is produced by shallow water nonlinear effect outside the estuary, the other is generated by shallow water nonlinear effect inside estuary. In addition, the physical mechanism of the residual tidal current and     

柴达木盆地昆特依盐湖的地球化学演化与古气候变化

１９９０年用铀系年代学及古地磁对昆特依盐湖沉积物进行年代测定，探讨该湖区地球化学演化与古气候变化关系。结果表明，该区最老的盐层形成于早更新世晚期（大于７３０ｋａ），大量的盐类沉积则始于３００ｋａ左右；盐湖沉积物中元素含量变化主要受古盐度、矿物、类质同象和吸附控制；沉积物水溶Ｆ／Ｃｌ比值与古盐度成反比关系；该湖２０００ｋａ以来的古环境演变：盐层形成于干冷、偏酸性的氧化环境，泥岩层形成于湿暖、偏碱性的     

Characteristic analysis on oxygen isotopic tracer in the East China Sea and waters to east of the Ryūkyū-guntō

Samples of O isotopic tracer were mlleMed at Sections P₃,P₂₅,P_cM-t/2-E and P_CM-1/2.w in both the Fast China Sea and the area to the east of the Ryūkyū-gunto during October-November,1991.Analytical results of the δ¹⁸O are as follows: (1) In the Kuroshio area,the δ¹⁸O isolines are almost parallel to the 200 m isobath.The value of δ¹⁸O is negative and reaches minimum mt the main axis of the Kuroshio,and increases on both sides.(2) In the Taiwan Warm Current (TWC) area there is a high δ¹⁸O tongue extending to the northeast.(3) In the area near the coast,the distribution of δ¹⁸O isoline shows that the Changjiang River runoff diffuses seaward and the land-ocean isotopic effect from the nearshore to the offshore.(4) The values of δ¹⁸O are from -1.0×10^-3 to -0.5×10^-3 in the shelf.(5) There is a low mre of δ¹⁸O value(<-1.6×10^-3) at the 600 m layer in the Kuroshio area,which is quite in accord with the existence of a low salinity mre (S G 34.30) between the 600 and 800 m layers in the same area.Finally,the mrrelations of the δ¹⁸O with the salinity and temperature,the upwelling and so on are discussed.     

The study of the Kuroshio in the East China Sea and the currents east of the Ryukyu Islands

In this study, the inverse method is used to compute the Kuroshio in the East China Sea and southeast of Kyushu and the currents east of the Ryukyu Islands, on the basis of hydrographic data obtained during September-October, 1987 by R/V Chofu Maru. The results show that: (1)A part of the Taiwan Warm Current has a tendency to converge to the shelf break; (2) the Kuroshio flows across the section C3 (PN) with a reduced current width, and the velocity of the Kuroshio at the section C3 increases and its maximum current speed is about 158 cm/s, and its volume transport here is about 26×106m3/s; (3) the Kuroshio has two current cores at the sections C3 (PN) and B2 (at the Tokara Strait); (4) the currents east of the Ryukyu Islands are found to flow northward over the Ryukyu Trench during September-October, 1987. The velocities of the currents are not strong throughout the depths. At the section C2 east of the Ryukyu Islands, the maximum current speed is at the 699 m levei and its magnitude is 25 cm/s, and i     

广东三水盆地晚自垩世—始新世古地磁研究及构造意义

对广东三水盆地上白垩统三水组、大　山组、古新统莘庄组及始新统　心组、宝月组的系统的古地磁研究结果表明：（１）旋转运动是该区构造运动的主要形式，顺时针旋转盆地拉张发育，这时针旋转盆地挤压衰亡，发育的程度与旋转角度有关；（２）始新世该区旋转方向由顺时针旋转变为道时针旋转，对应于太平洋－库拉板块对欧亚板块作用方向的改变，说明三水盆地的形成发育与太平洋－库拉板块对欧亚板块的作用密切相关；（３）三水盆地所在的华南地块从晚白垩世至始新世在纬向上不存在明显的运动，一直停留在略低于现今纬度的位置上。     

环太湖河道污染物负荷量的初步研究

根据１９８７－１９８８年环太湖河道水质，水量实测资料以及环湖主要河道的流量和水质的历史资料，结合平原水网地区的水情特征，提出了环太湖河道污染物负荷量的计算方法，并分析探讨了不同设计水情下环太湖河道污染物负荷量及其时空变化。结果表明，污染物负荷量与水情关系密切，枯水年的河道入湖污染物负荷量大于丰水年。环湖２２条主要河道的入湖污染物负荷量占全部河道入湖总量的７２％－９１％，且不同污染物入湖负荷量的分布     

Numerical Study on Instantaneous Discharge of Unsorted Particle Cloud in Cross Flow

The mixing characteristics of particles such as dredged sediment of variable size discharged into cross flow are studied by a 3D numerical model, which is developed to model the particle-fluid two-phase flow. The Eulerian method with the modified k-ε parameterization of turbulence for the fluid phase is used to solve fluid phase, while a Lagrangian method for the solid phase (particles), both the processes are coupled through the momentum sources. In the model the wake turbulence induced by particles has been included as additional source term in the k-ε model; and the variable drift velocities of the particles are treated efficiently by the Lagrangian method in which the particles are tracked explicitly and the diffusion process is approximated by a random walk model. The hydrodynamic behavior of dumping a cloud of particles is governed by the total buoyancy of the cloud, the drag force on each particle and the velocity of cross-flow. The computed results show a roughly linear relationship between the displacement of the frontal position and the longitudinal width of the particle cloud. The particle size in the cloud and the velocity of cross flow dominate the flow behavior. The computed results are compared with the results of laboratory experiments and satisfactory agreement is obtained.