麻痹性贝毒PSP在紫贻贝体内的累积、转化与排出

于1998年9月在青岛鲁迅公园附近礁石区采集紫贻贝（Mytilus edulis)，采用实验室培养的方法，初步研究了塔玛亚历山大藻（Alexandrium tamarense,ATHK)产生的麻痹性贝毒（Paralytic Shellfish Poison,PSP)在其体内累积、转化与排出的规律。结果表明，在累积实验阶段，紫贻贝内脏的和肌肉中的PSP毒素含量均随实验时间的延长而逐渐增加，累积实验结束时，平均每只贝体内的PSP毒素含量为13.40nmol，毒性水平为12.24ugSTXEq/100g，紫贻贝内脏中的毒素含量远远高于肌肉，内脏中PSP毒素占贝体内PSP毒素总量的97.5%。在8天的排出实验阶段，贝体内的PSP毒素总量呈下降趋势，实验结束时，PSP毒素共排除了约50%，每天排除率约为9%。     

北黄海沉积物——水界面反硝化速率及影响因素研究

探讨近海氮的循环机制,采用乙炔抑制法和现场静态箱法对北黄海夏季局部海域的反硝化速率进行了研究,该海域反硝化速率在2.5~5.8μmol/m2.h之间,平均4.85μmol/m2.h。影响其反硝化速率的主要因素为溶解氧,其次是温度。北黄海的反硝化速率低于珠江口和长江口海域。     

声波在水-多孔介质海底界面上的反射与透射

声波在海底界面的反射和透射是海底散射、海底混响、海底目标探测的重要问题。利用Biot多孔介质声传播理论对声波在水-多孔海底界面上的反射和透射进行了分析,具体给出反射声波的反射系数,3种透射声波的透射系数以及声能透射系数随入射波入射角和频率(10~40 kHz)的变化关系,分析了各种透射波对透射声能的贡献。多孔海底介质参数分别采用Stoll和Chotiros给出的2组参数进行理论计算。     

温度和盐度骤变条件下凡纳滨对虾的行为变化研究

以视频记录分析的方法,研究凡纳滨对虾Litopenaeus vannamei在温度和盐度骤变条件下的运动行为、摄食行为及相关生理活动的变化。结果表明,当温度由29℃骤降至18和23℃时,对虾的活动频率、游走速度和颚舟片的摆动频率明显下降,索饵时间显著增加(p<0.05);当温度由29℃骤升至36℃时,对虾的活动频率、游走速度和颚舟片的摆动频率显著升高(p<0.05),索饵时间没有显著差异(p>0.05)。当盐度由30骤降至5和10时,对虾表现出强烈的逃避行为,活动频率、游走速度和颚舟片的摆动频率均显著升高(p<0.05),并且在观察时间内没有发生摄食行为。     

福建漳浦前湖湾有孔虫群落及其沉积环境

对福建漳浦前湖湾潮间带两个钻孔岩心和表层沉积物中的有孔虫进行分析研究,有孔虫出现在钻孔最上部厚约2.46-2.96m的砂层中.根据群落优势种生态和非种特征判断,该段各样品有孔虫沉积环境均为近岸浅水环境,尽管其间海面有过3次上下波动变化.根据地层关系判断,其当为现代的产物.     

Numerical and simplified methods for the calculation of the total horizontal wave force on a perforated caisson with a top cover

The VOF method and the k–ε model, combined with the equation of state of air at constant temperature, have been used to calculate the total horizontal wave force caused by monochromatic waves acting on a perforated caisson with a top cover. From comparison of various parameters, such as the total horizontal force, the pressure difference on the front wall, the pressure on the back wall and the pressure on the top cover, between the numerical results and test data, it can be seen that the numerical results agree well with the test data. It is concluded that the method described in this paper can be utilized to calculate wave forces acting on perforated caissons with a top cover in the case of nonovertopping, nonbreaking waves. A simplified method to calculate the total horizontal force has been developed, based on test data, using a least-squares method. A comparison between the numerical results and the values calculated from the simplified equations shows good agreement. Therefore the simplified equations can be used in engineering applications to evaluate the total horizontal force on a perforated caisson with a top cover.     

一种灰树花菌丝体多糖GF4F1的结构特征及其抗肿瘤活性

以灰树花发酵菌丝体为原料，经过提取分离纯化得到1种水溶性多糖GF4F1，经高碘酸氧化、Smith降解、甲基化、红外光谱（IR）以及核磁共振波谱（NMR）等分析表明，该多糖是1种β-1, 3／1，6-葡聚糖，且小鼠体内实验结果表明其具有良好的抗肿瘤活性。在1mg／kg时，对小鼠抑制率为67.3％。     

96孔酶标板法测定中国对虾血淋巴上清液抗菌活力和酚氧化酶活性的初步研究

用96孔酶标板法测量单尾中国对虾血淋巴上清液抗菌（Uα）应力和过氧化酶活性。实验表明，该法具有以下优点：1）可测定大批单尾对虾血淋巴上清液的抗菌活力，酚氧化酶活性；2）所需对虾血淋巴上清液微量化；3）用酶标仪读取酶的活性，减少了不同试验者人为读数时的误差；4）重复性高。     

ISOLATION OF DNA FROM SEA URCHIN SPERM NUCLEI BY HYDROXYAPATITE CHROMATOGRAPHY

This article describes in detail the procedures in obtaining spermatids from living animals and in isolating sperm nuclei DNA by hydroxyapatite chromatography. The isolated DNA is free of RNAs and proteins after treatment with proteinase K, followed by chromatography on hydroxyapatite column which is eluted with stepwise-increased concentrations of potassium phosphate buffer. More than 89% of the DNA purified is found in the eluates of both 0.2 M and 0.25 M potassium phosphate buffer. The two fractions are identical in ultraviolet absorption spectrum. DNA molecules thus prepared should be useful for studying DNA structure and DNA replication by electron microscopy, since the length of the purified DNA molecules is in the range of the size of a replicating unit.     

不同软基处理方法适用性比较

总结分析了国内外现有的软土地基处理的各种方法。通过对采用不同方法进行软基处理的工程实例处理效果的定量对比，发现不同软基处理方法的适用性差异很大，多数工程中仅用一种方法很难达到设计要求，而两种或多种方法联合则可望取得更好的效果。