重组质粒HF443-EGFP的构建及其在赤潮毒素检测中的应用初探

采用基因工程的方法，进行重组质粒HF443一EGFP的构建和在哺乳动物细胞中的表达，并进行了其用于赤潮毒素检测的可能性研究。结果表明，用PCR技术扩增EGFP基因，用XbaⅠ、Hind Ⅲ双酶切并纯化，与同样双酶切并纯化的HF443质粒连接，可构建重组质粒HF443-EGFP；利用Lipofect AMINE2000转染体外培养哺乳动物BIU-87细胞。在培养体系中加入不同浓度的GTX，而后加入Na^ 通道激活剂乌头碱以诱导绿色荧光蛋白表达，荧光显微镜下观察细胞中绿色荧光蛋白表达的变化情况。细胞发出的荧光随毒素浓度的增加而减弱，存在一定的剂量效应关系，提示毒素GTX可显著影响绿色荧光蛋白的表达，重组质粒：HF443-EGFP用于赤潮霉素检测有一定的可行性.     

基于Argo浮标数据的水文特征三维可视化分析——以南大洋印度洋扇区为例

利用南大洋2009—2020年的Argo浮标温盐剖面数据,通过绘制温度和盐度空间分布图的定性分析以及三维聚类可视化分析等方法,揭示了南大洋印度洋扇区的多水团空间分布特征.结果表明,在60°S～67°S,60°E～80°E之间的海域,海水温度在-1.9～2.5℃之间,盐度在32～34.75 psu之间.整个研究区域海水温...     

Vertical profiling of SO2 and SO above Venus’ clouds by SPICAV/SOIR solar occultations

New measurements of sulfur dioxide (SO₂) and monoxide (SO) in the atmosphere of Venus by SPICAV/SOIR instrument onboard Venus Express orbiter provide ample statistics to study the behavior of these gases above Venus’ clouds. The instrument (a set of three spectrometers) is capable to sound atmospheric structure above the clouds in several observation modes (nadir, solar and stellar occultations) either in the UV or in the near IR spectral ranges. We present the results from solar occultations in the absorption ranges of SO₂ (190–230 nm, and at 4 μm) and SO (190–230 nm). The dioxide was detected by the SOIR spectrometer at the altitudes of 65–80 km in the IR and by the SPICAV spectrometer at 85–105 km in the UV. The monoxide’s absorption was measured only by SPICAV at 85–105 km. We analyzed 39 sessions of solar occultation, where boresights of both spectrometers are oriented identically, to provide complete vertical profiling of SO₂ of the Venus’ mesosphere (65–105 km). Here we report the first firm detection and measurements of two SO₂ layers. In the lower layer SO₂ mixing ratio is within 0.02–0.5 ppmv. The upper layer, also conceivable from microwave measurements by Sandor et al. (Sandor, B.J., Todd Clancy, R., Moriarty-Schieven, G., Mills, F.P. [2010]. Icarus 208, 49–60) is characterized by SO₂ increasing with the altitude from 0.05 to 2 ppmv, and the [SO₂]/[SO] ratio varying from 1 to 5. The presence of the high-altitude SO_x species could be explained by H₂SO₄ photodissociation under somewhat warmer temperature conditions in Venus mesosphere. At 90–100 km the content of the sulfur dioxide correlates with temperature increasing from 0.1 ppmv at 165–170 K to 0.5–1 ppmv at 190–192 K. It supports the hypothesis of SO₂ production by the evaporation of H₂SO₄ from droplets and its subsequent photolysis at around 100 km.     

Sulfur chemistry in the middle atmosphere of Venus

Venus Express measurements of the vertical profiles of SO and SO₂ in the middle atmosphere of Venus provide an opportunity to revisit the sulfur chemistry above the middle cloud tops (～58 km). A one dimensional photochemistry-diffusion model is used to simulate the behavior of the whole chemical system including oxygen-, hydrogen-, chlorine-, sulfur-, and nitrogen-bearing species. A sulfur source is required to explain the SO₂ inversion layer above 80 km. The evaporation of the aerosols composed of sulfuric acid (model A) or polysulfur (model B) above 90 km could provide the sulfur source. Measurements of SO₃ and SO (a¹Δ → X³Σ^-) emission at 1.7 μm may be the key to distinguish between the two models.     

长水国际机场红土动力特性室内试验研究

为了探究长水国际机场红土在动力荷载作用下的动力特性,通过开展一系列固结不排水动三轴试验对红土在动力荷载作用下的动变形特性进行研究.变形试验结果表明:随着围压的增大,动应力也增大,固结比越大,试样达到相同变形程度所需要的动应力越大;在相同的应变条件下,动弹性模量随着固结围压增大而增大;固结围压、固结比越大,阻尼比越大.达...     

“全域-局部”不透水面信息遥感分步提取模型

提出"全域-局部"遥感信息分布提取模型,通过计算和整合影像局部范围内的空间和光谱特征来优化全域上光谱混淆较大像元的提取精度。模型分为两个主要计算步骤:"全域"前分类与"局部"后分类;"全域"前分类将仅划分出满足一定精度阈值标准的像元,而"局部"后分类则在此部分分类结果基础上,进一步发掘和计算已分类像元所蕴含的信息来辅助对全域未分类像元的提取。在不透水面专题提取过程中,采用支持向量机SVM作为前分类器,通过控制精度阈值所对应的分类后验概率产生部分分类结果;采用调节最小距离分类器作为后分类器,根据一定的权重整合像元局部范围内的空间与光谱信息,代替了传统的全域光谱信息来优化分类。实验采用TM5影像以及所对应的NLCD(National Land Cover Data)标准不透水面产品作为测试集,"全域-局部"模型对应单一SVM模型的提取精度由80.31%提高为82.73%,局部后分类器精度较单一SVM模型由54.27%提高到59.94%。实验证明该模型具有较明显的精度提升且能够较好地解决不透水面与裸土混淆的问题,并得到空间形态上更为完善的不透水面提取结果。     

Stability of solution for dynamic geodetic network adjustment

This paper discusses theoretically the stability of solutions for dynamic geodetic network adjustment, Kalman filtering and sequence adjustment, and two examples are given. The solution for dynamic geodetic network adjustment is stable if the dynamic geodetic network is a classical network. There is not rank deficit in datum, or else the solution is not stable, which will depend on the initial value.     

Unification and harmonization in true color image city map

True color image city map is a sort of new-style map which combines the high resolution image and map symbols and shows both advantages in visualization. At the same time, the map unification and harmonization should be taken into account during the design process, since some visual conflicts appear when map symbols overlaid on the true color image. The objective of this research is to explore the rules in the process of true color image city map design based on chromatic and aesthetic knowledge. At the end, taking the Image Atlas of Guangzhou as an example, image color adjustment, road network presentation, and symbol designing issues will be discussed in the application.     

Estimating the sensitivity of runoff to climate change in an alpine-valley watershed of Xinjiang,China

ABSTRACT

The spatial-temporal variation of runoff in an inland basin is very sensitive to climate change. Investigation of runoff change in arid areas is typically limited by lack of meteorological and hydrogeological data. This study focused on runoff change in the Yarkand River source area of the Tarim Basin, China, with the aim of analysing the influence of climate change on the response characteristics of discharge. Sensitivity analysis was introduced to reflect the degree of influence of climate on runoff. Based on the sensitivity factors, over 30 sets of schemes including the IPCC Fourth Assessment Report were simulated using the MIKE 11/NAM rainfall–runoff model and the response of runoff was analysed. The results indicate that there are significant correlations and synchronous fluctuations between runoff and precipitation, evaporation and temperature. The characteristics of the sensitivity of runoff can be fitted well by Bi-Gaussian functions. The functions show that high sensitivity indexes mainly appear in the interval of 165 ± 100 m³ s^-1. The influence of precipitation on runoff is greater than that of other climate factors. Through simulation using the NAM model, we found that change of annual runoff was related to the initial climate condition. Annual runoff will have an increasing trend if it has a strong sensitivity to the initial meteorological condition. Moreover, the runoff decreases linearly with evaporation. Also it has a positive relationship with temperature and precipitation. Across the four seasons, the impact in summer and winter is greater than that in spring and autumn. Estimation of the spatial-temporal influence of climate on runoff could provide insight for water resource development in arid areas.

Editor Z.W. Kundzewicz Associate editor not assigned