Methods for extraction of microorganism DNA from glacier surface snow

In order to thoroughly investigate the diversity of glacier microorganisms, four DNA extraction methods with different lysis patterns were tested and two screened methods (the Bosshard-Bano method and the Zhou method) were optimized for the most effective form of the filter membrane (cut vs. uncut), the DNA extraction method, and the precipitation method. The two optimized methods were then compared with the commercial Mo-Bio DNA extraction kit, and the results showed that the kit was generally suitable for extraction of microorganism DNA from glacier surface snow. Procedurally, it was found that a modified Bosshard- Bano method (i.e., cutting the filter membrane into pieces, using a specific lysis pattern [lysozyme (5 mg/mL)-protease K (1 mg/mL)-CTAB (1%)-SDS (1%)], performing the extraction only once by chloroform-isoamyl alcohol (24:1), and conducting DNA precipitation by pure ethanol) was also an effective and less expensive method for extraction of microorganism DNA from glacier surface snow.     

2021年5月21日云南漾濞MS6.4地震序列地震矩张量及发震构造

2021年5月21日云南大理州漾濞县发生了MS6.4 地震.我们利用区域地震台网记录的地震波形资料,首先采用多点源地震矩张量反演方法确定了漾濞地震序列中 3 次MS≥5.0地震的矩心矩张量解,分析研究了地震矩释放的最佳模型;然后对序列中较大地震进行了绝对定位,结合余震序列重新定位结果研究了地震矩心在断层面上的位置.结果显示MS5.6 前震可用2 点源模型模拟,矩震级分别为MW 5.3、MW5.1,矩心时间相隔约30 s,矩心位置相距约 2 km.MS 6.4 主震可用单点源模型模拟,矩心与起始破裂点平面距离约 5 km.前震和主震的矩心均位于地表以下 6 km处,矩心与起始破裂点的位置关系显示两地震向南东方向单侧破裂,断层以"前震-主震"型地震序列典型的"撤退式"方式破裂,MS5.6 前震的发生降低了断层面的抗剪强度,从而发生了更大的MS6.4 主震.MS5.2余震可用单点源模型模拟,起始破裂点与矩心空间位置相近,在地表以下约 10 km处.余震区构造应力场反演结果显示漾濞 6.4级地震序列属于区域应力场触发的地震活动,地震序列震源机制解符合走滑断裂伴生的负花状构造系统内部断裂的运动特征,余震的空间分布图像显示花状构造系统内部的两条断裂发生了地震活动.     

内蒙碾子沟钼矿床辉钼矿Re-Os同位素年龄及其地质意义

内蒙古碾子沟石英脉型钼矿床是一新发现的钼矿床,通过对主要钼矿体6件辉钼矿样品的Re-Os同位素分析,获得了150.2±2.2~154.4±2.2Ma之间、平均152.4±1.3Ma（2σ误差,MSWD=1.6）的同位素模式年龄,及一个相关性很好的等时线年龄154.3±3.6Ma（2σ误差,MSWD=1.9）,表明碾子沟钼矿床形成于晚侏罗世,属中国东部燕山期大规模钼成矿期成矿,是中国东部中生代构造体制大转折背景下的产物。碾子沟钼矿床辉钼矿的铼含量介于12.6×10^-6~37.0×10^-6,平均24.9×10^-6,通过与其它钼矿床对比,初步认为碾子沟钼矿床成矿物质具有壳幔混源、偏壳源特征。     

The movement features analysis of Laohugou Glacier No. 12 in Qilian Mountains

The Laohugou Glacier No. 12 is the largest valley glacier in Qilian Mountains, which is located in northern Qinghai-Tibet Plateau. Movement is the basic characteristic of glaciers, and is also an important distinction from other terrestrial natural ice. Glacier changes not only reflect climate change, but also play an important role in humanity society. In the arid regions of western China, glaciers are becoming an important water source. We use the GPS receiver (South-Lingrui S82) as data platform with the aid of RTK measurement technology to observe the surface velocity of Laohugou Glacier No. 12. Surface velocity data shows that the maximum value appears at an altitude of 4,750–4,850 m during the period of 2008–2009. During this period, the west branch surface velocity reached 32.6 m per year at an altitude near 4,830 m, the east branch surface velocity reached 32.4 m per year at the altitude near 4,770 m. Comparing the surface velocity data during 2008–2009 with observation results in 1959, the glacier velocity slowed down about 11%.     

花岗岩源岩问题——关于花岗岩研究的思考之四

花岗岩源岩是花岗岩研究中最令人关注的问题之一,许多花岗岩的地球化学性质和分类实际上反映的是花岗岩的源岩问题.花岗岩幔源、壳源和壳幔混合源的说法被证明是不合适的,花岗岩不可能是幔源的,花岗岩都是壳源的,既然没有了幔源,也就无所谓壳幔混合源,因此,壳源本身也失去了意义.流行的花岗岩源岩组分混合计算的方法缺少理论依据,可变的因素太多,计算的结果可能没有多少实际意义.作者指出,对花岗岩来说第1位重要的是源区特征,它决定了花岗岩的基本面貌;其次是部分熔融程度、压力、温度和挥发分加入的情况;岩浆混合的意义可能是第3位的;而结晶分离作用可能是没有地位的.文中按照Nd-Sr同位素比值将花岗岩大致分为3个源区:即B、C和BC源区.B源主要由洋壳组成,C源主要由陆壳组成,BC源是二者的过渡.BC源区的组成很复杂,可能包括由交代地幔部分熔融形成的中基性岩浆岩,也可能是基性岩混染了陆壳物质的产物.B源与BC源可能与地幔亏损程度有关,B源来自强烈亏损的地幔,一部分BC源可能源于大陆下的富集地幔.文中还概略地讨论了中国各地花岗岩复杂的源区情况,指出中国花岗岩具区域性分布的特点说明花岗岩主要受源岩的制约.     

The mass-balance characteristics and sensitivities to climate variables of Laohugou Glacier No. 12, western Qilian Mountains, China

Due to global warming, glaciers on the Tibetan Plateau(TP) are experiencing widespread shrinkage; however, the mechanisms controlling glacier variations across the TP are still rather unclear, especially on the northeastern TP. In this study, a physically based, distributed surface-energy and mass-balance model was used to simulate glacier mass balance forced by meteorological data. The model was applied to Laohugou No. 12 Glacier, western Qilian Mountains, China, during2010~2012. The simulated albedo and mass balance were validated and calibrated by in situ measurements. The simulated annual glacier-wide mass balances were-385 mm water equivalent(w.e.) in 2010/2011 and-232 mm w.e. in 2011/2012,respectively. The mean equilibrium-line altitude(ELA) was 5,015 m a.s.l., during 2010~2012, which ascended by 215 m compared to that in the 1970 s. The mean accumulation area ratio(AAR) was 39% during the two years. Climatic-sensitivity experiments indicated that the change of glacier mass balance resulting from a 1.5 °C increase in air temperature could be offset by a 30% increase in annual precipitation. The glacier mass balance varied linearly with precipitation, at a rate of130 mm w.e. per 10% change in total precipitation.     

Assessing artificial neural networks coupled with wavelet analysis for multi-layer soil moisture dynamics prediction

Soil moisture simulation and prediction in semi-arid regions are important for agricultural production,soil conservation and climate change.However,considerable heterogeneity in the spatial distribution of soil moisture,and poor ability of distributed hydrological models to estimate it,severely impact the use of soil moisture models in research and practical applications.In this study,a newly-developed technique of coupled(WA-ANN) wavelet analysis(WA) and artificial neural network(ANN) was applied for a multi-layer soil moisture simulation in the Pailugou catchment of the Qilian Mountains,Gansu Province, China.Datasets included seven meteorological factors:air and land surface temperatures,relative humidity,global radiation, atmospheric pressure,wind speed,precipitation,and soil water content at 20,40,60,80,120 and 160 cm.To investigate the effectiveness of WA-ANN,ANN was applied by itself to conduct a comparison.Three main findings of this study were:(1) ANN and WA-ANN provided a statistically reliable and robust prediction of soil moisture in both the root zone and deepest soil layer studied(NSE >0.85,NSE means Nash-Sutcliffe Efficiency coefficient);(2) when input meteorological factors were transformed using maximum signal to noise ratio(SNR) and one-dimensional auto de-noising algorithm(heursure) in WA, the coupling technique improved the performance of ANN especially for soil moisture at 160 cm depth;(3) the results of multi-layer soil moisture prediction indicated that there may be different sources of water at different soil layers,and this can be used as an indicator of the maximum impact depth of meteorological factors on the soil water content at this study site.We conclude that our results show that appropriate simulation methodology can provide optimal simulation with a minimum distortion of the raw-time series;the new method used here is applicable to soil sciences and management applications.     

地表温度对太阳常数变化响应的数值试验研究

通过改变太阳常数，利用NCAR气候系统模式CSM1．4就地表温度对强外辐射强迫变化的响应及性质进行了研究。结果表明：虽然局地的增温幅度变化很大，但各试验的全球增温分布特征非常相似，并从一定程度上反映了全球增暖典型试验中的增温分布特点，即陆地比海洋增暖幅度更强，高纬度地区比低纬度地区增暖幅度更强，这一特点在太阳常数增加较大的试验中表现尤为明显。气候系统响应的性质在太阳常数分别增加2，5％、10％和15％与增加25％之间其响应方式有所改变，即气候系统对较小太阳常数变化的响应是线性的，而对较大太阳常数变化的响应则很可能是非线性的。     

被动源面波和体波成像在内蒙古浅覆盖区勘探应用

地震勘探具有勘探深度和分辨率的优势,在矿产勘探中多被采用.但主动源反射地震具有成本高、在矿区采集困难等难题,限制了其广泛应用.无需主动源激发、利用天然噪声的被动源地震应用于勘探,可成为其低成本替代选项.本文在内蒙古浅覆盖区矿区进行了被动源勘探试验,采用相关计算获得拟炮集记录,并基于频率域信噪比计算,在生成拟炮集前实现了面波和体波甄选,分离了面波和体波.应用面波反演的横波速剖面识别了覆盖层厚度.体波数据经反射波常规处理,获得了成像剖面.经与主动源反射剖面对比,主要结构的反射特征呈现了良好的一致性.本文试验验证了被动源勘探在内蒙浅覆盖区具有良好应用前景,为低成本的面积性勘探实施提供了新的选择.     

岩浆热场：它的基本特征及其与地热场的区别

"岩浆热场"指的是由岩浆引发的瞬间热场。热场的热主要来自未固结的岩浆,岩浆加热了围岩,使下地壳、中地壳和上地壳的下部在一个短暂的时间内保持一种高热状态。岩浆热场与地热场有许多不同:(1)热的来源不同。地热场的热主要来自地壳物质放射性生成的热;岩浆热场的热来自岩浆。(2)热的分布不同。地热场的等温面总体上呈水平分布,温度随深度增加而增加;岩浆热场的等温面则围绕岩体分布,靠近岩体温度高,远离岩体温度低,故岩浆热场的等温面是大体垂直于地热场等温面分布的。(3)热场的规模不同。地热场是全球性的,岩浆热场是局部性的,只在有岩浆的地方才出现。岩体小则规模小(热场宽度仅几米或几十米),岩体大则规模大(宽约几千米);如果存在大规模岩浆活动,岩浆热场的长宽均可达几百或上千千米,如在中国东部中生代大规模岩浆活动期间。(4)热持续的时间不同。地热场可以持续很长的时间(几十、几百或几千个百万年);岩浆热场是瞬间的突发性事件,持续的时间从几年到几个百万年。岩浆热场最重要的意义是,它是热液赖以上升的通道,它有利于来自下地壳底部和壳幔过渡带的流体(热液)的活动,使含矿热液得以顺利上升,并在热场范围内进行充分的活动、对流循环、萃取围岩中的成矿金属元素,并在地壳浅部岩浆热场之上合适的部位沉淀富集成矿。"岩浆热场"的概念依赖于对岩浆物理性质和过程的深入了解,由于我们这方面的知识相对贫乏,所以目前对岩浆热场的了解还是很肤浅的。