Fast genetic mapping in barley: case studies of cuticle mutants using RNA-sequencing

Barley(Hordeum vulgare L.) is one of the earliest domesticated crop species and ranked as the fourth largest cereal production worldwide. Forward genetic studies in barley have greatly advanced plant genetics during the last century; however, most genes are identified by the conventional mapping method. Array genotyping and exome-capture sequencing have also been successfully used to target the causal mutation in barley populations, but these techniques are not widely adopted because of associated costs and partly due to the huge genome size of barley. This review summarizes three mapping cases of barley cuticle mutants in our laboratory with the help of RNA-sequencing. The causal mutations have been successfully identified for two of them and the target genes are located in the pericentromeric regions. Detailed information on the mapping-by-sequencing, mapping-and-sequencing, and RNA-sequencing assisted linkage mapping are presented and some limitations and challenges on the mapping assisted by RNA sequencing are also discussed. The alternative and elegant methods presented in this review may greatly accelerate forward genetics of barley mapping, especially for laboratories without large funding.     

Overview of In - Situ Biodegradation and Enhancement

Microbial degradation technologies have been developed to restore ground water quality in aquifers polluted by organic contaminants effectively in recent years. However, in course of the degradation, the formation of biofilms in ground water remediation technology can be detrimental to the effectiveness of a ground water remediation project. Several alternatives are available to a remedial design engineer, such as Permeable Reactive Barriers (PRBs) and in -situ bioremediation, Hydrogen Releasing Compounds (HRCs) barrier, Oxygen Releasing Compounds (ORCs) barrier etc. which are efficient and cost- effective technologies. Excessive biomass formation renders a barrier ineffective in degrading the contaminants, Efforts are made to develop kinetics models which accurately determine bio - fouling and bio - filn formation and to control excessive biomass formation.     

Variability of Soft X—ray Spectral Shape in Blazars Observed by ROSAT

1 INTRODUCTION Blazars, including BL Lac objects, highly polarized and optically violently variable quasars,and flat-spectrum radio quasars (FSRQs), are characterized by highly variable non-thermalemission which dominates their characteristics from radio to y-ray bands. The mechanismbelieved to be responsible for their broadband emission is synchrotron radiation followed by in-verse Compton (IC) scattering at higher energies (e.g. Blandford & Konigl 1979). Relativisticbeaming of a jet…     

大同—阳高Ms6.1地震前兆综合加权信息熵的变化

利用“地震预报计算机专家系统”的思想对大同 -阳高 Ms6 .1地震前每一前兆异常事件进行综合评估 ,以每一异常的最可能发震时间来计算发震概率 ,利用地震前兆综合加权信息熵研究了系统熵值与地震的关系。对华北地区的地震前兆综合加权信息熵研究表明 ,在大同 -阳高 Ms6 .1地震前 ,信息熵出现了明显的减熵有序变化     

CHANGE OF NUTRIENT IMPORT AND EXPORT IN PROCESS OF RAINFALL IN AILAO MOUNTAIN OF YUNNAN PROVINCE

ＣＨＡＮＧＥ　ＯＦ　ＮＵＴＲＩＥＮＴ　ＩＭＰＯＲＴ　ＡＮＤ　ＥＸＰＯＲＴ　ＩＮ　ＰＲＯＣＥＳＳ　ＯＦ　ＲＡＩＮＦＡＬＬ　ＩＮ　ＡＩＬＡＯ　ＭＯＵＮＴＡＩＮ　ＯＦ　ＹＵＮＮＡＮ　ＰＲＯＶＩＮＣＥＧａｎＪｉａｎｍｉｎ（甘健民）；ＸｕｅＪｉｎｇｙｉ（薛敬意...     

四个PG类星体的谱能结构与红外辐射机制

本文利用宽波段能谱结构讨论了四个PG类星体的红外辐射机制．结果表明，在产生红外连续辐射的可能机制中，非热的、具有幂律特征的辐射和被核光度加热的“核尘埃”的热再辐射是红外辐射的主要来源．对于高光度的活动星系核，它的主星系的红外辐射及恒星形成区的红外辐射是可以忽略的．     

Mobile Agent系统结构及其关键技术

Mobile Agent技术是一门新兴的技术,具有移动性、智能性和异步计算的特点,已成为计算机网络和分布式系统最具活力的发展方向.现对Mobile Agent的概念、系统结构及其关键技术进行了分析和探讨.     

图像目标形状匹配与视频过滤技术研究

通过研究图像目标形状特征的提取、匹配，运用随机过程，投影理论，提出用于识别目标形状统计特性的波动度特征，建立目标形状匹配函数，给出实际图像目标的匹配实现算法，实际系统测试表明该算法能准确、快速完成实际图像目标形状的匹配、过滤，为图像跟踪、识别及视频过滤等应用领域提供了新的可借鉴方法。     

基于ArcGIS的二次调查地籍图形拓扑检测

为了保证二次调查地籍数据库的质量，提高地籍空间数据检查效率，在分析二次调查地籍建库规范的基础上，提出了应用ArcGIS对地籍图形进行拓扑检测，并通过实例加以阐述。     

Zircon U–Pb age, trace element and Hf isotope composition of Kongling terrane in the Yangtze Craton: refining the timing of Palaeoproterozoic high-grade metamorphism

U–Pb age, trace element and Hf isotope compositions of zircon were analysed for a metasedimentary rock and two amphibolites from the Kongling terrane in the northern part of the Yangtze Craton. The zircon shows distinct morphological and chemical characteristics. Most zircon in an amphibolite shows oscillatory zoning, high Th/U and ¹⁷⁶Lu/¹⁷⁷Hf ratios, high formation temperature, high trace element contents, clear negative Eu anomaly, as well as HREE-enriched patterns, suggesting that it is igneous. The zircon yields a weighted mean ²⁰⁷Pb/²⁰⁶Pb age of 2857 ± 8 Ma, representing the age of the magmatic protolith. The zircon in the other two samples is metamorphic. It has low Th/U ratios, low trace element concentrations, variable HREE contents (33.8 ≥ Lu_N≥2213; 14.7 ≤ Lu_N/Sm_N ≤ 354) and ¹⁷⁶Lu/¹⁷⁷Hf ratios (0.000030–0.001168). The data indicate that the zircon formed in the presence of garnet and under upper amphibolite facies conditions. The metamorphic zircon yields a weighted mean ²⁰⁷Pb/²⁰⁶Pb age of 2010 ± 13 Ma. These results combined with previously obtained Palaeoproterozoic metamorphic ages suggest a c. 2.0 Ga Palaeoproterozoic collisional event in the Yangtze Craton, which may result from the assembly of the supercontinent Columbia. The zircon in two samples yields weighted mean two-stage Hf model ( T _DM2) ages of 3217 ± 110 and 2943 ± 50 Ma, respectively, indicating that their protoliths were mainly derived from Archean crust.