Flow cytometry and pigment analyses as tools to investigate the toxicity of herbicides to natural phytoplankton communities

Characterisation of natural phytoplanktonic communities is currently being advanced through flow cytometry and high resolution pigment analyses. To date, toxicological methods to assess impacts of herbicides on natural phytoplankton populations are lacking. Here, we report the novel use of these techniques in combination to study changes in phytoplankton populations exposed to 2-methylthio-4-tertiary-butylamino-6-cyclopropylamino-s-triazine (Irgarol 1051), a herbicide used in antifouling paints. Flow cytometry results revealed that following a 72-h exposure to approximately 100 ngL(-1), eukaryote abundance was less than half that in the controls. High performance liquid chromatographic analyses of pigments demonstrated that 19'-hexanoyloxyfucoxanthin was selectively lost relative to the control. This carotenoid is specific to the prymnesiophytes which are key constituents of phytoplanktonic communities within temperate marine waters. Values of EC(50) (72 h) as low as 70 ngL(-1) were calculated from the selective reduction in this compound. Concentrations substantially exceeding this level have been reported in UK and other European coastal waters.     

An upper limit to the acetylene abundance toward BN in the Orion molecular cloud

We report a search for the acetylene (C2H2) nu 3 infrared vibration-rotation absorption near 3 micrometers toward the Becklin-Neugebauer source in the Orion molecular cloud. The relative abundance of C2H2/CO in the quiescent gas (9 km s-1 component) is less than 3 x 10(-3) (5 sigma).     

推广应用多工艺空气钻进技术成效显著

多工艺空气钻进技术是当今世界范围内最有发展前景和应用广泛的钻探与施工技术之一。近１０余年来,地矿部门在开发研究与推广应用方面取得了丰硕成果。主要表现在完成“七五”期间地矿部重点科技攻关项目多工艺空气钻进技术研究与开发（编号：８６０１９）所取得的一批科...     

Elements in fish of Malibu Creek and Malibu Lagoon near Los Angeles,California

Our aim was to assess whether past discharges from a wastewater treatment plant increased metal pollutant loads in stream mobile species in a one-day baseline sampling study that included a coastal wetland. Mosquitofish (Gambusia affinis) of two sizes, black bullhead (Ameiurus melas), and crayfish (Pacifastacus leniusculus) were collected from Malibu Creek, and California killifish (Fundulus parvipinnis) of three sizes, as well as arroyo chub (Gila orcutti) were sampled from Malibu Lagoon near Los Angeles, California. Species from each locality were pooled by length, homogenized, digested by microwave wet ashing, and analyzed by simultaneous inductively coupled plasma atomic emission spectroscopy for 27 elements. Lagoon killifish 2.0-3.5 cm long contained levels of arsenic and lead above the levels for 95% of California fish, the EDL95. Black bullhead upstream of the discharge contained elevated levels of As, Cr and Se. Young mosquitofish <3 cm in length upstream of the discharge differed greatly in the order of abundance of their elements relative to larger mosquitofish and to other species collected. More sampling than this baseline study allowed was needed to determine if the wastewater treatment plant was a pollution source.