乳糖诱导重组别藻蓝蛋白(rAPC)在大肠杆菌(Escherichia coli)中的表达

采用廉价的乳糖替代IPTG诱导重组别藻蓝蛋白(rAPC)在大肠杆菌(Escherichiacoli)JM109中表达,对诱导产物表达的培养基、培养条件、诱导剂的浓度和诱导时机进行了优化,将优化条件用于5L自控发酵罐进行高密度培养。结果表明,对rAPC表达的条件进行优化后,乳糖诱导rAPC的表达率可达26.8%,与IPTG的诱导结果接近;在高密度培养中,OD600最大达21.8。研究结果可为乳糖替代IPTG大规模生产rAPC奠定基础。     

荧光法在赤潮图像分析中的应用

赤潮藻样品中往往包含着各种复杂的非藻沉淀物,为解决这些微藻的探测和分析的问题,可以利用微藻类在一定波长的光照射下会产生荧光的特性,对裸甲藻等多种赤潮藻类进行荧光实验处理,并在此基础上对藻类的荧光图像和灰度图象进行图像分割,轮廓和纹理分析及图象识别等后继图象处理。实验证明,荧光法可以有效的去除背景噪声,保留藻类信息,使得后继的图象处理工作更加容易和有效,从而解决了探测和分析包含复杂背景的沉淀物样品中微藻的问题。     

Impacts of TRMM SRR assimilation on the numerical prediction of tropical cyclone

1 IntroductionObservation of the tropical rainfall is crucial forthe research on tropical weather and climate. Nu-merous studies have shown that the ingestion of rain-fall data into a numerical model can have considera-ble impacts on simulation results(Kr…     

Ecopath with Ecosim在水生生态系统研究中的应用

地球上的水体为人类提供着无法计量的生态系统服务和相应的生态价值。然而,水生生态系统一直承受着巨大的捕捞和环境压力。为更好地分析人类以及自然扰动对水生生态系统的影响,并建立科学合理的管理机制,许多研究种群结构和动态的模型软件以     

海洋蓝藻基因组学研究

1994年,日本Kazusa实验室首先启动了淡水蓝藻Synechocystissp.PCC6803的基因组计划,并于1996年完成基因组测序的工作。Synechocystissp.PCC6803基因组大小为3.57 Mb,共有3 168个基因,是目前研究最多也最为透彻的蓝藻基因组。除此之外,美国能源部下属的联合基因组研究所(JointGe     

不同季节的企鹅珍珠贝游离珠植核效果比较

在雷州流沙港湛江海洋大学珍珠研究室实验基地分别于春、夏和秋季进行了企鹅珍珠贝游离珠植核实验。其中，春季插核的死亡率最低，为15.1％--16．2％，留核率最高，为10％左右：而夏季插核的死亡率最高，为63．56％，留核率也仅为2．22％：秋季插核的留核率最低，仅收珠1颗，留核率为0．35％，死亡率也较高，为54.4％。结果表明，春季是企鹅珍珠贝较适宜的插核季节。     

仿鱼尾潜器推进系统的水动力分析

以开发适用于小型潜器的仿生操纵与推进系统为研究背景 ,对金枪鱼的月牙形尾鳍进行水动力分析。文中将金枪鱼的尾鳍处理为在做横移和摇摆的耦合运动的同时 ,以某一匀速向前运动的月牙形刚性尾翼。计算中应用了双曲面元和压力库塔条件 ,利用面元法计算分析该三维尾翼的非定常水动力性能。探讨了前进速度、横荡和摇首的幅度、频率及其相位差对推进性能的影响     

水面皮层破坏-复原过程的实验室模拟研究

依据水面红外发射和红外遥感测温原理，采用HDG－高灵敏度红外测温仪和常规测量仪器相结合的方法，在实验室空气稳定条件下，模拟测得了水面皮层破坏－复原（重建）的热力过程和气－水温差对水面皮层复原过程的影响，获得了大量的测量数据。数据分析表明，当气－水温差从3．0℃变为11．5℃时，水面皮层破坏可导致皮温增量从气－水温差3．0℃时的0．15℃变到11．5℃时的0．45℃，并发现恢复时间与气－水温差呈负线性关系。     

A new efficient 3D non-hydrostatic free-surface flow model for simulating water wave motions

A new three-dimensional, non-hydrostatic free surface flow model is presented. For simulating water wave motions over uneven bottoms, the model employs an explicit project method on a Cartesian the staggered gird system to solve the complete three-dimensional Navier–Stokes equations. A bi-conjugated gradient method with a pre-conditioning procedure is used to solve the resulting matrix system. The model is capable of resolving non-hydrostatic pressure by incorporating the integral method of the top-layer pressure treatment, and predicting wave propagation and interaction over irregular bottom by including a partial bottom-cell treatment. Four examples of surface wave propagation are used to demonstrate the capability of the model. Using a small of vertical layers (e.g. 2–3 layers), it is shown that the model could effectively and accurately resolve wave shoaling, non-linearity, dispersion, fission, refraction, and diffraction phenomena.     

Wave interaction with a perforated wall breakwater with a submerged horizontal porous plate

This study examines the hydrodynamic performance of a new perforated-wall breakwater. The breakwater consists of a perforated front wall, a solid back wall and a submerged horizontal porous plate installed between them. The horizontal porous plate enhances the stability and wave-absorbing capacity of the structure. An analytical solution based on linear potential theory is developed for the interaction of water waves with the new proposed breakwater. According to the division of the structure, the whole fluid domain is divided into three sub-domains, and the velocity potential in each domain is obtained using the matched eigenfunction method. Then the reflection coefficient and the wave forces and moments on the perforated front wall and the submerged horizontal porous plate are calculated. The numerical results obtained for limiting cases are exactly the same as previous predictions for a perforated-wall breakwater with a submerged horizontal solid plate [Yip, T.L., Chwang, A.T., 2000. Perforated wall breakwater with internal horiontal plate. Journal of Engineering Mechanics ASCE 126 (5), 533–538] and a vertical wall with a submerged horizontal porous plate [Wu, J.H., Wan, Z.P., Fang, Y., 1998. Wave reflection by a vertical wall with a horizontal submerged porous plate. Ocean Engineering 25 (9), 767–779]. Numerical results show that with suitable geometric porosity of the front wall and horizontal plate, the reflection coefficient will be always rather small if the relative wave absorbing chamber width (distance between the front and back walls versus incident wavelength) exceeds a certain small value. In addition, the wave force and moment on the horizontal plate decrease significantly with the increase of the plate porosity.