Second-Order Wave Diffraction Around 3-D Bodies by A Time-Domain Method

A time-domain method is applied to simulate nonlinear wave diffraction around a surface piercing 3-D arbitrary body. The method involves the application of Taylor series expansions and the use of perturbation procedure to establish the corresponding boundary value problems with respect to a time-independent fluid domain. A boundary element method based on B-spline expansion is used to calculate the wave field at each time step, and the free surface boundary condition is satisfied to the second order of wave steepness by a numerical integration in time. An artificial damping layer is adopted on the free surface for the removal of wave reflection from the outer boundary. As an illustration, the method is used to compute the second-order wave forces and run-up on a surface-piercing circular cylinder. The present method is found to be accurate, computationally efficient, and numerically stable.     

Non-Simultaneous Failure of Ice in Front of Multi-Leg Structures

Because the multi-leg jacket structure is the major type of offshore structures in the Bohai Sea, the study of non-si-multaneous failure of i鏴 on multi-leg structures is important. However, the non-simultaneous failure has not been consid-ered in engineering design until now, obviously resulting in costly design and notable waste. To resolve this problem, this paper, by means of analysis of experimental data, calculates the coefficient of the non-simultaneous failure for the double-pile structure, the square four-leg structure, the single-line multi-pile structure, and the conical structure, respectively, and provides some reference criteria for engineering design.     

The Structure of A Turbulent Jet in A Crossflow-Effect of Jet-Crossflow Velocity

A comprehensive numerical study on the three-dimensional structure of a turbulent jet in crossflow is performed. The jet-to-crossflow velocity ratio (R) varies in the range of 2 - 16; both vertical jets and inclined jets without excess streamwise momentum are considered. The numerical results of the Standard two-equation k-ε model show that the turbulent structure can be broadly categorised according to the jet-to-crossflow velocity ratio. For strong to moderate jet discharges, i.e. R> 4, the jet is characterized by a longitudinal transition through a bent-over phase during which the jet becomes almost parallel with the main freestream, to a sectional vortex-pair flow with double concentration maxima; the computed flow details and scalar mixing characteristics can be described by self-similar relations beyond a dimensionless distance of around 20-60. The similarity coefficients are only weakly dependent on R. The cross-section scalar field is kidney-shaped and bifurcated, vvith distinct double concentr     

Regression Formulations for the Stress at Hot Spot of Multiplanar Tubular DT Joints

- Stress concentration analysis of multiplanar tubular DT joints plays an important role in the fatigue design of offshore platforms. A semi-analytic method for stress analysis under the condition of any loads is briefly introduced in the paper. Nineteen multiplanar tubular DT joints with one of two braces of the same dimension subjected to axial loads and out- of- plane bending moments are computed for parametric stress analysis by using the present method. The influence of geometrical parameters on the stresses of multiplanar tubular DT joints is discussed and compared with corresponding uniplanar T joints. The regression formulae for the stress at hot spot of multiplanar DT joints are found by modification of SCF of corresponding uniplanar T joints. The parametric formulae for the maximum stress by superposition. Finally, the influences of stiffening effect and load-interaction effect on the maximum stress of DT joints are discussed.     

乳糖诱导重组别藻蓝蛋白基因在大肠杆菌中的表达

以重组别藻蓝蛋白表达工程菌JM109（DE3）／pET28-APC作为研究对象，对于JM109（DE3）菌株在乳糖诱导下表达重组蛋白的规律进行了研究。比较分析了不同生长阶段进行诱导，最佳乳糖浓度、诱导持续时间和诱导温度等参数对重组蛋白表达的影响。实验结果表明，采用JM109（DE3）为宿主菌，经过条件优化，乳糖诱导重组蛋白的表达量可以达到IPTG诱导的水平；较低的诱导培养温度能有效提高可溶性重组蛋白的表达。最后在摇瓶发酵结果的基础上，实现了乳糖诱导工程菌的5L全自动发酵罐培养。研究结果为在大肠杆菌大规模发酵中，运用廉价、无毒的乳糖作为诱导剂提供了一定的借鉴。     

Effect of Sea Level Variation on Tidal Characteristic Values for the East China Sea

1 .IntroductionTheglobalairtemperatureroseabout 0 .5～ 0 .6°Coverthepast 2 0thcentury ,andtheglobalmeansealevelincreasedbyabout2 0cmduringtheperiod .Theregionalmeansealevelriseswiththerisingglobalmeansealevel.Zuoetal.( 1 997)indicatedthatthemeanrisingrateofabsolutemeansealevelalongtheChinacoastontheassumptionofunifiedisostaticdatumis 2mm a .Woodworth( 1 999)analyzedsealevelspanning 1 76 8tothepresentinLiverpool,andobtainedaseculartrendforheperiodupto 1 880of0 .39± 0 .1 7mm a ,andatrendfort…     

Hilbert Transferin Applied to Separation of Waves

The analytical method (AM) for separation of composite waves is presented based on the Hilbert transform. It is ap-plicable to both regular and irregular trains of waves. The wave data series measured with two wave gauges in the experi-ments are separated into two series of incident and reflected waves. Then, the reflection coefficient can be easily ob-tained. The arrival of reflected waves can also be detected for improveraent of the accuracy of the reflection coefficient. The reflection performance of the physical model can be estimated exactly without calculation of wave height and phase difference. Numerical samples developed to test the method are proved to be accurate. Physical experiments are conduct-ed and compared with Goda s method and satisfactory results are obtained.     

Estimation of Water Particle Velocities by Empirical Transfer Function

-In previous and this studies it appears that the linear and nonlinear wave theory can notaccurately and easily predict the water particle velocities.Therefore,different from the theoretical consider-ations,in this study we have attempted to determine the transfer function empirically.Laboratory experi-ments were performed under various wave conditions.The empirical formulas of the transfer function ofthe wave height,angular frequency and water particle velocity were obtained on the basis of these test databy dimensional analysis and regression analysis.In intermediate and deep water depth conditions,thetransfer function was only a function of a nondimensional parameter which is composed of the angular fre-quency,the depth of the velocity gauge under the still water level,water depth and the acceleration of grav-ity.Finally,the empirical formulas were compared with experimental data and observational data formpresent and Cavaleri's(1978)studies.The empirical formulas were found to be in sufficient correl     

Mathematical Models for Combined Refraction-Diffraction of Waves on Non-Uniform Current and Depth

Two mathematical models for combined refraction-diffraction of regular and irregular waves on non-uniform current in water of slowly varying topography are presented in this paper. Model I is derived by wave theory and variational principle separately. It has two kinds of expressions including the dissipation term. Model n is based on the energy conservation equation with energy flux through the wave crest lines in orthogonal curvilinear coordinates and the wave kinematic conservation equation. The analysis and comparison and special cases of these two models are also given.     

石斑鱼虹彩病毒SGIV在宿主细胞内依赖微管运动的行为特征

病毒是非常微小的简单生物, 不能独立生存, 必须借助宿主细胞完成自身的繁衍。病毒侵染进入细胞后, 通常借助于微管通过黏稠的细胞质运动到特定的复制位点。然而, 有关病毒依赖微管运动行为的精细动态研究还比较少。石斑鱼虹彩病毒(Singapore grouper iridovirus, SGIV)为虹彩病毒科蛙病毒属的一个新种, 是海水养殖鱼类的重要病毒性病原, 对海水养殖业造成重大经济损失。利用单粒子示踪技术实时追踪了SGIV病毒粒子沿微管运动的行为, 观察到SGIV在细胞边缘至微管中心之间的双向运动, 最高瞬时速度约0.2μm·s^-1, 均表现为主动运输。病毒粒子运动至微管交叉位置会减速迂回, 而后或受限于此, 平均运动速率约0.008μm·s^-1, 或通过交叉处继续快速运动, 最高瞬时速度为0.2μm·s^-1。同时, SGIV感染会影响微管的形态结构, 随着SGIV感染, 微管逐渐围绕细胞核和病毒加工厂形成环状结构。研究结果初步揭示了SGIV病毒和细胞微管之间相互作用的复杂过程, 丰富了我们对虹彩病毒胞内生命活动的认识, 有助于深入地理解海水鱼类虹彩病毒感染致病机理。