Production of the Blood Pressure Lowing Peptides from Brown Alga （ Undaria pinnatifida ）

Brown alga （ Undaria pinnatifida） was treated with alginate lyase and hydrolyzed using 17 kinds of proteases and the inhibitory activity of the hydrolysates for the angiotensin-I-converting enzyme （ACE） was measured. Four hydrolysates with potent ACE-inhibitory activity were administered singly and orally to spontaneously hypertensive rats （SHRs）. The systolic blood pressure of SHRs decreases significantly after single oral administration of the brown alga hydrolysates by pro- tease S ＇ Amano＇ （from Bacillus stearothermophilus） at the concentration of 10 （mg protein） （kg body weight）^ - 1. In the 17 weeks of feeding experiment, 7-week-old SHRs were fed standard diet supplemented with the brown alga hydrolysates for 10 weeks. In SHRs fed 1.0 and 0.1% brown alga hydrolysates, elevating of systolic bloodpressure was significantly suppressed for 7 weeks. To elucidate the active components, the brown alga hydrolysates were fractionated by 1-butanol extraction and HPLC on a reverse-phase column. Seven kinds of ACE-inhibitory peptides were isolated and identified by amino acid composition analysis, sequence analysis, and LC-MS with the results Val-Tyr, Ile-Tyr, Ala-Trp, Phe-Tyr, Val-Trp, Ile-Trp, and Leu-Trp. Each peptide was determined to have an antihypertensive effect after a single oral administration in SHRs. The brown alga hydrolysates were also confirmed to decrease the blood pressure in humans.     

How the space astronomy has extended the horizon of astronomy

On the basis of my experience in the X-ray astronomy, and on some typical highlights of multi-wavelength observations, I emphasize the importance of collaboration between space astronomy and ground-based astronomy.Paper presented at the Symposium on the JNLT and Related Engineering Developments, Tokyo, November 29–December 2, 1988.     

Mineralogical and geochemical characteristics of hydrothermal alteration of basalt in the Kuroko mine area, Japan: implications for the evolution of a Back Arc Basin hydrothermal system

Basalt in the Furutobe District of the Kuroko mine area in Japan is characterized by abundant chlorite and epidote. Fluid inclusion studies indicate that chlorite is formed at lower temperatures (230–250°C) than epidote (250–280°C). The seawater/basalt mass ratio for the early chlorite-rich alteration was high (max. 40), but that for the later alteration was low (0.1–1.8). The CaO, Na₂O and SiO₂ of the bulk rock correlate negatively with MgO, while FeO and Σ Fe correlate positively with MgO. These changes in the characteristic features of hydrothermal alteration from early to late are generally similar to those for a mid-ocean ridge geothermal system accompanying basalt alteration.The MgO/FeO ratios of chlorite and actinolite and the Fe₂O₃ concentration of epidote from the basalt are greater than those of mid-ocean ridge basalt probably owing to the differences in the Fe₂O₃/FeO and MgO/FeO ratios of the parent rocks. The lower CaO concentration and the higher Na₂O concentration of the bulk rock compared with altered mid-ocean ridge basalt can be interpreted in terms of the difference in original bulk rock compositions.The Furutobe basalt, as well as other submarine back arc basalts, contains more vesicles filled with hydrothermal minerals (epidote, calcite, quartz, chlorite, pyrite) than do the mid-ocean ridge basalts. The abundance of vesicles plays an important role in controlling the secondary mineralogy and geochemistry of hydrothermally altered submarine back arc basin basalts.     

Research cooperation forever

Journal of Oceanology and Limnology -     

Constraining sediment provenance for tsunami deposits using distributions of grain size and foraminifera from the Kujukuri coastline and shelf,Japan

Tsunami deposits preserved in the geological record provide a more comprehensive understanding of their patterns of frequency and intensity over longer timescales; but recognizing tsunami deposits can prove challenging due to post-depositional changes, lack of contrast between the deposits and surrounding sedimentary layers, and differentiating between tsunami and storm deposition. Modern baseline studies address these challenges by providing insight into modern spatial distributions that can be compared with palaeotsunami deposits. This study documents the spatial fingerprint of grain size and foraminifera from Hasunuma Beach and the Kujukuri shelf to provide a basis from which tsunami deposits can be interpreted. At Hasunuma Beach, approximately 50 km east of Tokyo, the spatial distribution of three common proxies (foraminiferal taxonomy, foraminiferal taphonomy and sediment grain size) for tsunami identification were mapped and clustered using Partitioning Around Medoids cluster analysis. Partitioning Around Medoids cluster analysis objectively discriminated two coastal zones corresponding to onshore and offshore sample locations. Results show that onshore samples are characterized by coarser grain sizes (medium to coarse sand) and higher abundances of Pararotalia nipponica (27 to 63%) than offshore samples, which are characterized by finer grain sizes (fine to medium sand), lower abundances of Pararotalia nipponica (2 to 19%) and Ammonia parkinsoniana (0 to 10%), higher abundances of planktonics (15 to 58%) and species with fragile tests including Uvigerinella glabra. When compared to grain-size and foraminiferal taxonomy, foraminiferal taphonomy; i.e. surface condition of foraminifera, a proxy not commonly used to identify tsunami deposits, was most effective in discriminating modern coastal zones (identified supratidal, intertidal and offshore environments) and determining sediment provenance for tsunami deposits at Kujukuri. This modern baseline study assists the interpretation of tsunami deposits in the geological record because it provides a basis for sediment provenance to be determined.     

A pollution history of Chesapeake Bay

Present day anthropogenic fluxes of some heavy metals to central Chesapeake Bay appear to be intermediate to those of the southern California coastal region and those of Narragansett Bay. The natural fluxes, however, are in general higher. On the bases of Pb-210 and Pu-239 + 240 geochronologies and of the time changes in interstitial water compositions, there is a mixing of the upper 30 or so centimeters of the sediments in the mid-Chesapeake Bay area through bioturbation by burrowing mollusks and polychaetes. Coal, coke and charcoal levels reach one percent or more by dry weight in the deposits, primarily as a consequence of coal mining operations.     

The densest meteorite collection area in hot deserts: The San Juan meteorite field (Atacama Desert,Chile)

Abstract– We describe the geological, morphological, and climatic setting of the San Juan meteorite collection area in the Central Depression of the Atacama Desert (Chile). Our recovery activities yielded 48 meteorites corresponding to a minimum of 36 different falls within a 3.88 km² area. The recovery density is in the range 9–12 falls km^?2 depending on pairing, making it the densest among meteorite collection areas in hot deserts. This high meteorite concentration is linked to the long‐standing hyperaridity of the area, the stability of the surface pebbles (> Ma), and very low erosion rates of surface pebbles (approximately 30 cm Ma^?1 maximum). The San Juan meteorite population is characterized by old terrestrial ages that range from zero to beyond 40 ka, and limited weathering compared with other dense collection areas in hot desert. Chemical weathering in San Juan is slow and mainly controlled by the initial porosity of meteorites. As in the Antarctic and other hot deserts, there is an overabundance of H chondrites and a shortage of LL chondrites compared with the modern falls population, suggesting a recent (< few ka) change in the composition of the meteorite flux to Earth.     

Nutrient and Phytoplankton Dynamics in Harima-Nada, Eastern Seto Inland Sea, Japan During a 35-Year Period from 1973 to 2007

Long-term monitoring of water quality and phytoplankton was conducted at 19 sampling stations in Harima-Nada, eastern Seto Inland Sea, Japan for 35 years from 1973 to 2007. There were two significant long-term changes, an increase in winter water temperatures of 0.042°C year^?1, and a decrease in dissolved inorganic nitrogen (DIN) from about 10 μM in the 1970s to ~5 μM in the late 1990s due to the reduction in nutrient inputs. DIN concentrations and total phytoplankton cell density were both higher during the 1970s to the early 1980s and then exhibited a significant decrease in the mid 1980s and remained relatively constant thereafter. Diatoms were the dominant phytoplankton group (>90%) over the 35-year period, and there was a dramatic shift from Skeletonema dominance (~70%) to Chaetoceros in the mid 1980s. This shift in diatom species may be attributed to differences in the life cycle of Skeletonema and Chaetoceros and the response to the decrease in DIN concentration.     

Reliability analysis of shallow foundations in reference to design codes development

In this study, FORM analysis is carried out for shallow foundation of a typical building in order to evaluate relative magnitude of uncertainties involved in the force and the resistance sides. The distinguished feature of this study is to take into account the uncertainty of the earthquake force as much as possible. Tthis is because, the seismic design dominates major part of structural design in Japan, and without referring to this problem, no useful information of practical value is gained. The results of the FORM analysis exhibited the reliability index values between 2.0 to 3.5, which is considered to be in a reasonable range. Since the 50 year maximum earthquake force is considered in the reliability calculation, calculated reliability index values are for the period of 50 years. The sensitivity factor obtained in a case more realistic ground variability is taken into account exhibited values not very different from those obtained in the upper structures.