THE ULTRASTRUCTURE OF SEPARATED AND CULTURED CELL OF PORPHYRA YEZOENSIS

There are many reports that cells (protoplasts) separated from the thallus of Porphyra by enzyme can develop to normal leafy thalli in the same way as monopores. But there are few investigations on the subcellular structure of the isolated vegetative cell for ccmparison with the subcellular structure of monospores. To clarify whether the separated and cultured cells undergo the same or similar ultrastructure changes during culture and germination as monospores undergo in their formation and germination, we observed their ultrastructure, ccxnpared them with those of the monospore and found that the ultrastructure of separated and cultured cells did not have the characteristic feature as that of monospore formarion, such as production of small and large fibrous vesicles, but was acccxnpanied by vacuolation andstarch mobilization like that in monospore germination. The paper also discusses the relations between monospores and separated and cultured cells.     

南海北部一百万年以来的表层古生产力变化：来自ODP1144站的蛋白石记录

通过南海北部ODP 1144站蛋白石含量测定及其堆积速率的计算,并结合氧同位素记录等相关资料,获得南海北部1050ka以来高分辨率的表层古生产力变化与冰期旋回和东亚季风的关系。约900ka以来,蛋白石含量及其堆积速率较900ka以前明显增加,反映了“中更新世革命”事件之后,全球气候变冷,并导致表层生产力的提高。由于第四纪冰期旋回中的冬、夏季风的加强,加上1144站特殊的地理位置,使该站在冰期时表层生产力增加,间冰期时表层生产力降低。浮游有孔虫氧同位素记录与蛋白石含量及其堆积速率的时间序列频谱分析结果显示,三者均出现了相对应的偏心率周期、斜率周期和岁差周期,说明该站表层生产力的变化主要受地球轨道周期的驱动。     

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Deep Fractionation of Clinopyroxene in the East Pacific Rise 13°N:Evidence from High MgO MORB and Melt Inclusions

Mid-ocean ridge basalts(MORBs)from East Pacific Rise(EPR)13°N are analysed for major and trace elements,both of which show a continuous evolving trend.Positive MgO-Al_2O3 and negative MgO-Sc relationships manifest the cotectic crystallization of plagioclase and olivine,which exist with the presence of plagioclase and olivine phenocrysts and the absence of clinopyroxene phenocrysts.However,the fractionation of clinopyroxene is proven by the positive correlation of MgO and CaO.Thus,MORB samples are believe...     

海上E区天然气水合物地质、地震、地球化学特征综合研究与成藏远景预测

本文提出一种将地质、地震、地球化学分析相结合的方法,以研究海区天然气水合物的成藏条件,首先用物源、构造、沉积和地温等资料进行地质分析,确定海区天然气水合物成藏的地质条件;然后根据对水合物的地震属性（BSR,AVO,BZ等）分析和正演模拟研究,预测水合物及其游离气的存在,结合地质条件,圈定出水合物成藏的有利区带;最后结合对水合物主要成分（甲烷及烃类等）的地球化学异常分析,对研究区天然气水合物成藏的远景作出评价．野外实践结果表明,这种综合研究方法是有效可行的,为海区天然气水合物勘探提供了一种新的研究途径．     

Heavy mineral stratigraphy of sediments from the southern outer shelf of the East China Sea since the last glaciation using fuzzy C-means cluster method

Correspondence analysis and fuzzy C-means cluster methods were used to divide the stratigraphy of heavy mineral assemblages,and the sediment sources and depositional dynamics of the environment reconstructed.The assemblages were taken from marine sediments from the late Pleistocene to the Holocene in Core Q43 situated on the outer shelf of the East China Sea.Based on the variable boundaries of the mineral assemblage at 63 and 228 cmbsf (cm below sea floor),the core might have previously been divided into th...     

Environmental anomalies in the northeastern East China Sea during the last 3 000 years:implications for El Nio activity in the Holocene

To reconstruct the productivity changes for the last 10 500 a in the northeastern East China Sea (ECS),biogenic compounds (such as carbonate,organic carbon and opal),marine micropaleontological fossils (planktonic foraminifera,benthic foraminifera,radiolarian and silicoflagellate) and the compositional characters of benthic foraminifera fauna analyses were carried out on a sediment core DOC082 obtained from the western slope of Okinawa Trough (29°13.93'N,128°08.53'E;1 128 m water depth).The long-term changes of biogenic and micropaleontological proxies display some similarities through the last 10 500 a,which show three different phases:lower values are recorded during the early and middle Holocene (before about 4 000 a BP),followed by an abrupt and remarkable increase at about 4 000 a BP,the late Holocene (after about 3 000 a BP) is characterized by continuously high values.The multi-proxy data of paleoproductivity and percents of benthic foraminifera genera (Uvigerina and Bulimina) show that during the early and middle Holocene (10 500-4 000 a BP) productivity was relatively low with a sudden and distinct increase at about 4 000 a BP,and the late Holocene (3 400-0 a BP) is marked by significantly higher productivity.Also,the radiolarian-based sea surface temperature (SST) records reveal a distinct decline in SST in the late Holocene after 3 200 a BP,very different from the early and middle Holocene.For the last 3 000 a,the enhanced biological productivity and distinctly lower SST indicate a major change of oceanographic conditions in the northeastern ECS.These marine environmental anomalies are consistent with other paleoclimatic records for the late Holocene in the Chinese continent and its surrounding regions.After analyzing the mechanisms of modern productivity and SST changes in the northeastern ECS,and based on the climatic anomalies in the Chinese continent and variations in the Kuroshio Current during modern El Nio periods,we suggest that the anomalous environmental conditions in the northeastern ECS may imply intensified El Nio activity during the late Holocene.     

Anti-proliferative activity of phlorotannin extracts from brown algae Laminaria japonica Aresch

In this study,we evaluated the anti-proliferative activity of phlorotannins derived from brown algae Laminaria japonica Aresch extracts on the human hepatocellular carcinoma cell (BEL-7402) and on murine leukemic cells (P388) by MTT assay.Cells were incubated with 100 μg/mL of the phlorotannin extract (PE) for 48 h.The inhibitory rate of PE on BEL-7402 and P388 cells was 30.20±1.16% and 43.44±1.86%,respectively,and the half-inhibitory concentration of PE (IC50) on P388 and BEL-7402 cells was 120 μg/mL and >...     

Analysis of expressed sequence tags from the Ulva prolifera (Chlorophyta)

In 2008,a green tide broke out before the sailing competition of the 29th Olympic Games in Qingdao.The causative species was determined to be Enteromorpha prolifera (Ulva prolifera O.F.Müller),a familiar green macroalga along the coastline of China.Rapid accumulation of a large biomass of floating U.prolifera prompted research on different aspects of this species.In this study,we constructed a nonnormalized cDNA library from the thalli of U.prolifera and acquired 10 072 high-quality expressed sequence tags ...     

De-eutrophication of effluent wastewater from fish aquaculture by using marine green alga Ulva pertusa

The de-eutrophication abilities and characteristics of Ulva pertusa, a marine green alga, were investigated in Qingdao Yihai Hatchery Center from spring to summer in 2005 by analyzing the dynamic changes in NH+4, NOˉ3, NO2ˉ as well as the total dissolved inorganic nitrogen (DIN). The results show that the effluent wastewater produced by fish aquaculture had typical eutrophication levels with an average of 34.3 μmol L-1 DIN. This level far exceeded the level IV quality of the national seawater standard and c...