ONTOGENIC STUDY OF ISOZYMES IN GIANT TIGER PRAWN,PENAEUS MONODON (CRUSTACEA:DECAPODA)

The patterns of isozyme variation throughout the life cycle of the giant tiger prawn, Penaeusmonodon were described for ten enzyme systems (G3PD, MDH, LGG, GPI, MPI, FBP, PGM, LDH,PGD, and PROT). The zymograms showh little variation in the number of isozymic loci expressed duringlarval development compared with results reported for other penaeid prawns. There were specific ontogenicpatterns at some loci. As larvae developed beyond the embryonic stage, there was tendency for intensityof staining and complexity of banding patterns to increase.     

Identification of Prorocentrum minimum and Takayama pulchella by fluorescence in situ hybridization through epifluorescence microscopy and flow cytometry

Partial rDNA sequences of Prorocentrum minimum and Takayama pulchella were amplified, cloned and sequenced, and these sequence data were deposited in the GenBank. Eight oligonucleotide probes (DNA probes) were designed based on the sequence analysis. The probes were employed to detect and identify P. minimum and T. pulchella in unialgal and mixed algal samples with a fluorescence in situ hybridization method using flow cytometry. Epifluorescence micrographs showed that these specific probes labeled with flu...     

High-Resolution Mesoscale Analysis Data from the South China Heavy Rainfall Experiment (SCHeREX): Data Generation and Quality Evaluation

In this study, the observational data acquired in the South China Heavy Rainfall Experiment (SCHeREX) from May to July 2008 and 2009 were integrated and assimilated with the US National Oceanic and Atmospheric Administration’s (NOAA) Local Analysis and Prediction System (LAPS; information available online at http://laps.fsl.noaa.gov). A high-resolution mesoscale analysis dataset was then generated at a spatial resolution of 5 km and a temporal resolution of 3 h in four observational areas: South China, Cent...     

Characterisation of rapid infiltration flows and vulnerability in a karst aquifer using a decomposed fluorescence signal of dissolved organic matter

The fluorescent properties of dissolved organic matter (DOM) have been used as natural tracers in various water systems. However, only few studies have focused on groundwater systems, specifically, on karst systems. The aim of this study was to develop the use of the DOM fluorescence signal as a natural tracer, considering the multiple compartments of a karst system. This method was applied to the Lez hydrosystem, which supplies the city of Montpellier with drinking water. The hydrodynamics and hydrochemistry of the spring were monitored beginning March 2006. The DOM fluorescence was measured by the excitation–emission matrix spectroscopy technique. The analysis of the total fluorescence signal confirms the efficiency of this tool to trace rapid infiltration flows. Moreover, the decomposition of the signal into different fluorophores complements the information provided by the total signal. Indeed, the fluorescence emitted by the humic compounds seems to be the ideal tool for identifying rapid infiltration flows. Nevertheless, the fluorescence of protein-like compounds is better correlated with the inflow of faecal bacteria at the outlet. This decomposition of the fluorescence signal is an interesting way to provide information on both the rapid infiltration flow as well as the vulnerability of the karst aquifers.     

Tectonic Evolution and Hydrocarbon Potential in Northern Area of the South Yellow Sea

The northern area of the South Yellow Sea, located in the offshore region of China, resulted from the continental-continental collision orogeny during the Mesozoic and can be divided into four stages in terms of tectonic evolution: (1) pre-orogenic passive continental margin stage (Z-T2); (2) foreland basin stage corresponding with the late phase of the Sulu (苏鲁) orogeny (J3-K); (3) post-orogenic intracontinental rifted basin stage (K2t-E); and (4) regional subsidence and coverage stage (N-Q). Based on deta...     

黔北桐梓五峰组观音桥段双壳类和腹足类的生态意义

黔北桐梓红花园地区的五峰组是华南地区奥陶系的重要剖面之一,在其以泥质灰岩为特征的五峰组观音桥段下部黑色含炭质钙质页岩和褐色粉砂岩中分别产有较为丰富的双壳类Modiolopsis和腹足类Holopea。产双壳和腹足类的地层中岩性和生物具有差异性,表明观音桥段沉积早期的沉积环境具有多变的特征。横长卵形、较膨凸的Modiolopsis和其两瓣壳相连保存的标本表明为近原地埋藏,为弱水动力、有氧的、较浅水环境的软质基底。丰度较大的低圆锥形Holopea的不规则的壳顶指向指示了具一定水动力的、沙性基底的浅水环境。     

Inheritance of 15 microsatellites in the Pacific oyster Crassostrea gigas： segregation and null allele identification for linkage analysis

Microsatellites were screened in a backcross family of the Pacific oyster, Crassostrea gigas. Fifteen microsatellite loci were distinguishable and polymorphic with 6 types of allele-combinations. Null alleles were detected in 46.7% of loci, accounting for 11.7% of the total alleles. Four loci did not segregate in Mendelian Ratios. Three linkage groups were identified among 7 of the 15 segregating loci. Fluorescence-based automated capillary electrophoresis (ABI 310 Genetic Analyzer) that used to detect the microsatellite loci, has been proved a fast, precise, and reliable method in microsatellite genotyping.     

An event-by-event assessment of tropical intraseasonal perturbations for general circulation models

We detect and characterize each large-scale intraseasonal perturbation in observations (1979–2009) and in coupled general circulation models of Institut Pierre Simon Laplace (IPSL) and of Centre National de Recherches Météorologiques (CNRM). These ensembles of intraseasonal perturbations are used to assess the skill of the two models in an event-by-event approach. This assessment addresses: (1) the planetary-scale (i.e. the whole Indo-Pacific area) extent of wind and rainfall perturbations and the reproducibility of the perturbation patterns for a given season; (2) the size and amplitude of rainfall and wind anomalies at basin-scale (i.e. for a particular phase of the perturbation) and; (3) the evolution of the vertical structure of the perturbations (U, T and RH) for selected events. The planetary-scale extent of rainfall perturbations is generally too small for both models. This extent is also small for the wind perturbation in the IPSL model, but is correct, or even too large in boreal winter, for the CNRM model. The reproducibility of the planetary-scale patterns is exaggerated for wind perturbations in the CNRM model and is very poor for all parameters in the IPSL model. Over the Indian Ocean during boreal winter, rainfall and wind anomalies at basin-scale are too large for the CNRM model and too small for the IPSL model. The CNRM model gives a realistic baroclinic perturbations structure for wind, moisture and temperature, but with too large amplitude due in part to a zonally extended rainfall anomaly over the eastern Indian Ocean and the Maritime Continent. The IPSL model gives a realistic response for low-level wind only. Temperature and moisture perturbations are barotropic with a wrong warm anomaly at rainfall maximum and there is no gradual increase in low-level moisture prior to this rainfall maximum. These results suggest that this version of the IPSL model is unable to initiate the coupling between the convection and the dynamic necessary to develop the perturbation. It is difficult to say if this is due to, or is at the origin of the lack of basin-scale organization of the convection. We discuss the likely role of the convective schemes in the differences found between these two versions of the CNRM and IPSL models.     

海洋重力资料整理技术与软件实现

针对海洋重力测量成果规范化整理与重力异常计算软件开发问题,重点讨论了重力掉格校正与测线文件获取的可视化方法;引入贝塞尔方法计算船速和航向,并进行厄特渥斯校正.在此基础上,依据海洋重力调查规范,基于图形可视化技术开发了一套具有人机交互功能的海洋重力资料整理软件.