中华齿米虾胰蛋白酶基因的研究

以中华齿米虾(Neocaridina denticulata sinensis)基因组DNA为模板,根据胰蛋白酶基因保守序列设计简并引物,利用PCR技术获得一个基因。序列分析表明此基因与已报道的凡纳滨对虾(Litopenaeus vannamei)胰蛋白酶基因有较高的相似度,序列中包含一个内含子和两个不完整的外显子,编码182个氨基酸残基。该氨基酸序列与凡纳滨对虾胰蛋白酶氨基酸序列的相似度为83.5%;含有胰蛋白酶所特有的His、Asp和Ser组成的活性三联体、决定胰蛋白酶底物专一性的Asp、底物结合部位的G1y残基。综合分析认定该基因为胰蛋白酶基因片断。将其氨基酸序列与报道的其他动物胰蛋白酶氨基酸序列进行了系统进化分析。系统进化分析的结果与现有以表型特征为依据的虾分类结果是一致的。     

中国东海2万年来海平面变化分析

拟通过中国东海相对海平面与全球海平面对比分析，来获得东海构造沉降数据。但在对比分析的过程中发现，断裂作用对中国东海150m以深海域相对海平面的变化有重要影响。这种作用使东海150m以深海底至少下降了30m，这是我国学者先前误认为中国东海末次冰盛期最低海面位置位于现今海面下150～160m一带的主要原因。在此基础上，进一步对水与冰川重力均衡、沉积物压实和构造沉降作用进行了探讨。结果认为重力均衡不足、沉积物压实作用微弱，构造沉降是造成东海相对海平面与全球海面之间差异的又一重要原因。同时发现东海构造沉降随水深呈规律性变化。断裂作用的表现结果所赋予的更深层次的思考与认识是，在17kaB．P．以前，中国东海水深150～160m一带是1个相对隆起带，其存在对陆架区圈闭和捕获中国大陆古水系和沉积物具有重要意义。     

Mach Effect of Oblique Nonlinear Irregular Waves Interacting with Vertical Walls

Based on Hong‘s theory, previous random models, and a generalized expression suitable for FIT calculation, the interaction between irregular waves and vertical walls is numerically simulated. The results of simulation demonstrate that the wave energy changes with the incidence angle and the distance from the wall. Particularly, the Mach effect and the combined wave spectrum characteristics are analyzed in detail, which are significant in both theory and practice.     

Analysis of Dynamic Characteristics of Submarine Free Spanning Pipelines by Complex Damping Method

Considering the effect of the internal flowing fluid and the external marine environmental condition, the differential equation for the vortex-induced vibration (V1V) of the free spanning pipeline is derived and is discretized by the Hermit interpolation function. The free vibration equation with the damping term is solved by the complex damping method for the natural frequency, and then the effect of fluid damping on the natural frequency of the free spanning pipeline is analyzed.The results show that fluid damping has a significant influence on the damped natural frequency of the free spanning pipeline in the lock-in state, while it has little influence when the pipeline is out of the lock-in state. In the meantime,the change of the free span length has the same effect on the damped natural frequency and the undamped natural frequency.     

Optimization Mathematical Model of Pile Forces for Offshore Piled Breasting Dolphins

An optimization mathematical model of the pile forces for piled breasting dolphins in the open sea under various loading conditions is presented. The optimum layout with the well distributed pile forces and the least number of piles is achieved by the multiplier penalty function method. Several engineering cases have been calculated and compared with the result of the conventional design method. It is shown that the number of piles can be reduced at least by 10%—20% and the piles‘ bearing state is improved greatly.     

A Numerical Model of Coastal Processes of Sand Beaches Based on Long-Term Wave Series

A numerical model of shoreline change of sand beaches based on long-term field wave data is proposed, the explicit and implicit finite difference forms of the model are described, and an application of the model is presented. Results of the application indicate that the model is sensitive to the order of the input wave data, and that the effects of long-term wave series and the effects of the mean annual wave conditions on the model are different. Instead of a single wave condition, the wave series will make the calibration and the verification of the model more practical and the results of the model more reasonable.     

Observations and Estimations of Directional Spectrum of Sea Waves

The directional spectrum is one of the basic characteristics of sea waves. The observations of directional spectrum of sea waves were successfully conducted at platform Bohai 8 during 1991 and 1992 using a wave gage array for the first time in China. Based on the field data, the directional spectrum which depends on the wave growth is given in this paper. Before observations, the effects of the type of gage array, the distance between the gages and the platform itself on the measured results and the precision of some methods for estimating the directional spectrum were investigated and compared with the methods of numerical simulations and model tests of multi-direcitonal irregular waves. This ensures the quality of the observations and estimations of the directional spectrum.     

PLS-ANN分析血浆自体荧光光谱的二阶导数光谱识别胃癌

探讨偏最小二乘法结合神经网络法(简称PLS-ANN)分析血浆自体荧光光谱的二阶导数光谱识别胃癌的优势,对20例胃癌病人和23例健康人血浆进行以405nm为激发光的自体荧光光谱检测,采用PLS-ANN法分别对血浆自体荧光光谱和二阶导数光谱进行判别分析。PLS-ANN分析血浆的自体荧光光谱法诊断胃癌的灵敏度为75%,特异度为83%,准确率为79%,PLS-ANN分析血浆的二阶导数光谱法诊断胃癌的灵敏度为90%,特异度为96%,准确率为93%。结果表明PLS-ANN分析血浆的二阶导数光谱法识别胃癌,优于PLS-ANN分析血浆的自体荧光光谱法,有望成为快速识别胃癌的较理想方法。     

添加维生素B_（12）生产菌高密度培养褶皱臂尾轮虫的效果

阐述把维生素Ｂ１２生产菌添加到培养水中培养褶皱臂尾轮虫ＢｒａｃｈｉｏｎｕｓＰｌｉｃａｔｉｌｉｓ的买验。共１８株细菌分离于轮虫培养池，其中，有一株产维生素Ｂ１２的假单胞杆菌ＴＰ４对轮虫的生长繁殖有明显的促进作用。把ＴＰ４菌株培养后，加入到２Ｌ的烧杯和５００Ｌ的水槽中培养泰国Ｓ型轮虫时．在９ｄ（天）和６ｄ（天）中，轮虫密度从１２４～１３９和２４２～２８８个体／ｍｌ增殖到４，４１７～５，５４０和１，０１７～１，２５４个体／ｍｌ，分别比对照组增加了４～６及２～３倍。     

A Linear Programming Model for Seabed Oil-Gas Pipe Network

This paper analyzes the pipe network system of oil-gas collection and transportation foroffshore oilfield development.A"0-1"integer linear programming model is constructed to optimize theinvestment of seabed pipe network.The mathematical model is solved by the spanning tree method ofgraph theory and network analysis.All spanning trees of a network graph compose all the feasible solu-tions of the mathematical model.The optimal solution of the model is the spanning tree with the minimumcost among all spanning trees.This method can be used to optimize the seabed pipe network system andgive a minimum cost plan for the development of offshore marginal oilfield groups.