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41.
将青岛文昌鱼(Branchiostoma belcheri tsingtauense)肌球蛋白重链基因片段亚克隆到pET-30a质粒,构建出重组表达载体并转化入大肠杆菌Escherichia coli BL21中.经SDS-PAGE和Western杂交检测表明,在IPTG诱导下含有重组载体的菌株可表达分子质量约30 ku的融合蛋白.诱导条件优化实验结果显示,该菌株经0.2 mmol/L IPTG诱导1 h就可大量表达此蛋白.可溶性实验确认该重组蛋白是可溶性蛋白.本研究为文昌鱼肌球蛋白重链特异性抗体的制备奠定了基础. 相似文献
42.
Effect of Cyclic Loading Frequency on Undrained Behaviors of Undisturbed Marine Clay 总被引:2,自引:0,他引:2
Based on a series of cyclic triaxial tests, the effect of cyclic frequency on the undrained behaviors of undisturbed marine clay is investigated. For a given dynamic stress ratio, the accumulated pore water pressure and dynamic strain increase with the number of cycles. There exists a threshold value for beth the accumulated pore water pressure and dynamic strain, below which the effect of cyclic frequency is very small, but above which the accumulated pore water pressure and dynamic strain increase intensely with the decrease of cyclic frequency for a given number of cycles. The dynamic strength increases with the increase of cyclic frequency, whereas the effect of cyclic frequency on it gradually diminishes to zero when the number of cycles is large enough, and the dynamic strengths at different frequencies tend to the same limiting minimum dynamic strength. The test results demonstrate that the reasons for the frequency effect on the undrained soil behaviors are beth the creep effect induced by the loading rate and the decrease of sample effective confining pressure caused by the accumulated pore water pressure. 相似文献
43.
The vertical migration of diatoms in mud-flats was studied in littoral of Xibian, Longhai County and Xiao'ao, Lianjiang County, the dominant species being Gyrosigma spencerii and Hantzschia virgata respectively. Both light and tide are important factors affecting the vertical migration of diatoms. With the advent of tide, the diatom community disappears from the surface. It reappears gradually when exposed to light. In the dark, the diatoms never appear in great quantity on the surface in laboratory or in littoral. Whether in the light or in the dark, the diatoms aggregate in maximum numbers in the upper 2 mm of the sediment. But when the tide has covered the sediments for 4 hr, Hantz. virgata penetrates the mud down to a depth of 14 mm, Gy. spencerii, 12 mm. We have found that Gy. spencerii posseses phototaxis, but no tide rhythm and no day-night rhythm in our laboratory. 相似文献
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46.
The numerical mode of nonlinear wave transformation based on both the Laplace equation for water field and the Bemoulli equation for water surface is a kind of time-domain boundary problem with initial conditions. And the basis for establishing the numerical mode of nonlinear wave in time domain is to trace the position of wave free surface and to calculale the instantaneous surface height and surface potential function. This paper firstly utilizes the ‘0-1‘ combined BEM to separate the boundary by means of discretization of Green‘ s integral equation based on the Laplace equation, then separates the free surface of wave with FEM and derives the FEM equation of wave surface that satisfies the nonlinear boundary conditions. By jointly solving the above BEM and FEM equations, the wave potential and surface height could be obtained with iteration in time domain. Thus a new kind of nonlinear numerical mode is established for calculating wave transformation. The wave test in the numerical wave tank shows that the numerical simulation with this mode is of high accuracy. 相似文献
47.
Characteristics of Scour and Deposition in Front of Breakwaters Under Irregular Broken Clapotis 总被引:1,自引:0,他引:1
First the scour and deposition patterns of the sandy seabed in front of a vertical breakwater under the action of irregular broken clapotis are investigated experimentally and classified into five types: scour type Ⅰ , scour type Ⅱ, scour type Ⅲ, deposition type Ⅰ, and deposition type Ⅱ . Secondly, the processes of formation of scour and deposition patterns are probed in comparison with those induced by regular broken clapotis and standing waves. Thirdly, the criteria for distinguishing scour and deposition patterns under irregular broken clapotis are presented. 相似文献
48.
1 .IntroductionAshiptravelingatseaundergoesundesirablewave inducedmotions ,namely ,surge ,sway ,heav ing ,rolling ,pitchingandyaw .Thesemotionsoftencauseproblemstothecrew ,theonboardequip mentand ,intheworstcase ,thesafetyofthevessel.Tominimizethewave inducedshipmotions ,controlsystemsmaybeapplied .Theaccuratemodelingofshipmotionsisthereforeveryimportantforshipdesignanddesignofmotioncontrolsystems .Manyresearchershavedevelopedshipmotionpredictionmethodsbasedonthepotentialflowtheo ries (Dong ,… 相似文献
49.
Modal Wave Number Tomography for South China Sea Front 总被引:10,自引:0,他引:10
1 .IntroductionTheSouthChinaSealiestothesoutheastpartoftheChinesecontinent.TheKuroshioflowsbytheeastsideoftheLuzonStraitfromsouthtonorthinwinter ,spring ,andautumn .TheKuroshioaf fectsnearcurrentfieldsandtemperaturefields,changingtheoceanenvironmentparametersandsoundpropagation .Thus ,theoceanacoustictomographytechniqueisofpotentialtolong term ,largescalemonitoringoftheocean .Themodalwavenumbertomographymethod (Rajanetal.,1 987;Frisketal.,1 989)isusedtoobtainthesoundspeedprofileinwatercol… 相似文献
50.
1 .IntroductionNondestructiveinspection (NDI)isveryimportantforensuringthereliabilityofoffshorestructuresintheirservicelives (Lauraetal.,1 996 ) .Itiswellknownthatdetectionofflawsinvolvesconsider ablestatisticaluncertainties.Asaresult,theprobabilityofdetection (POD)forallflawsofagivensizehasbeenusedintheliteraturetodefinethecapabilityofaparticularNDItechniqueinagivenen vironment.SincethedataofPODusuallyscatterlargely ,itisdifficulttodeterminewhichmodelfitstheavailabledatabest.Thismodelun… 相似文献