蛤蜊科3种贝类16SrRNA基因片段及ITS2核苷酸序列分析

利用PCR技术分别扩增连云港及启东沿海蛤蜊科的西施舌（Coelomactra antiquata）、中国蛤蜊（Mactra chinensis）和四角蛤蜊（Mactra veneriformis）3种双壳贝的16SrRNA基因片段和ITS2核苷酸序列．测序后用DNAstar软件分析了核苷酸差异。结果显示：三种贝类16SrRNA基因片段长度相同，均为306bp（去除引物）．核苷酸存在多态性。共有45个变异位点，54个核苷酸发生了变异。全部为碱基置换。西施舌与中国蛤蜊此片段核苷酸的同源性为88．9％．与四角蛤蜊的同源性为88．6％．中国蛤蜊与四角蛤蜊的同源性为90．6％。三种蛤蜊ITS2序列分别为390bp（西施舌）、441bp（四角蛤蜊）和466bp（中国蛤蜊）。存在长度多态性．ITS2核苷酸差异分析结果显示．西施舌与中国蛤蜊的同源性为70．9％-71．1％，西施舌与四角蛤蜊的为70．5％-71．0％。中国蛤蜊与四角蛤蜊的同源性为88．1％-88．8％。ITS2序列分析结果与16SrRNA基因片段分析结果一致．2种分子分析法均显示中国蛤蜊与四角蛤蜊的亲缘关系近。     

Protective effect of topically applied polypeptide from Chlamys farreri against ultraviolet radiation-induced chronic skin damage in guinea pig

Polypeptide fromChlamys farreri (PCF), a topical polypeptide isolated fromChlamys farreri, was used in this experiment aimed to investigate the photoprotective effect of PCF against chronic skin damage induced by ultraviolet A (UVA) and ultraviolet B (UVB) radiation. The chronic ultraviolet-irradiated guinea pig model was established, and visible changes in the skin including wrinkling, sagging and erythema were observed. Malondialdehyde (MDA) and antioxidant enzymes including superoxide dismutase (SOD) and glutathione peroxidase (GSH-px) in the dorsal skin were determined using biochemical methods. The results showed: (1) PCF (5 % and 20%) could greatly protect the dorsal skin of guinea pig against wrinkling, sagging and erythema induced by UV radiation in a concentration-dependent manner. (2) PCF could reduce MDA formation in the dorsal skin caused by UV irradiation, while increasing the activities of SOD and GSH-px. (3) The differences among the PCF groups and UV model group were significant (P<0.05,P<0.01). These results indicated that topical application, of PCF provided broad solar UV spectrum photoprotection; and that the antioxidant property of PCF might play a role in photoprotection. Project No. 39970638 supported by the NSFC, and the Science and Technology Bureau of Qingdao (No. 2001-28-50).     

蜕壳素对凡纳滨对虾生长及表观消化率的影响

研究了外源性蜕皮激素对凡纳滨对虾生长和表观消化率的影响 ,实验时在基础饲料中分别添加 0 .1%、0 .2 %、0 .4 %的两种市售蜕壳素产品 ,制成 6种外源性脱壳素配合饲料 ,不添加的基础饲料作为对照组 ,试验在 2 6 0L玻璃钢循环水养殖系统中 ,每个剂量组设 3个重复 ,8周生长试验结果表明 :市售蜕壳素产品能有效地促进对虾生长 ,试验结束时 ,试验组虾体增重分别较对照组增加 16 .8%、7.5 2 %、5 .9%、11.4 %、2 9.0 7%、16 .5 3% ,但是凡纳滨对虾对饲料粗蛋白的表观消化率没有明显地提高 ,凡纳滨对虾对脂肪的表观消化率较低。推算凡纳滨对虾饲料中最佳蜕皮酮添加量为 6 0mg/kg。     

酶解法提取鱼油的工艺参数优化

利用蛋白酶酶解法从黄鳍金枪鱼（Thunnus albacares）加工的下脚料——鱼头中提取鱼油。以鱼油提取率和感官特征为指标，通过正交优化实验设计，获得了胰蛋白酶提取鱼油的最佳酶解工艺参数：酶解温度45℃，酶添加量1．5％．料液质量比1：1，酶解时间4h，酶解pH8。在该条件下．鱼油的提取率为4．34％，理化指标除过氧化值外均达到SC／T3502-2000的粗鱼油二级标准。多不饱和脂肪酸总含量高达38．47％,其中DHA和EPA的含量分别为23．63％和4．84％。     

胶东“玲珑-焦家式”金矿资源潜力与找矿

“玲珑焦家式”金矿的成岩成矿,从新太古代至中生代存在着继承性的成因关系,成矿具有长期性、多源性特点,但中生代燕山早期岩浆活动是主导成矿因素。区域化探成果显示胶东金矿直接矿源岩———郭家岭花岗岩金的丰度比原始矿源岩———早前寒武纪变质沉积岩系及侵入岩系金的丰度低1/2。郭家岭花岗岩面积在2 km2以上岩体定量估计金亏损总量与胶东金矿总量预测的资源量相当,从一个侧面展现了胶东金矿资源的潜力。望儿山金矿的找矿实践展示,焦家式金矿在1 000 m以下尚有巨大潜力,整个胶东金矿产区在3 000 m以上的资源潜力应在5000 t以上。     

我国个人所得税制度完善的法律思考

个人所得税是与每个人联系都很紧密的税种,它的完善与否,直接体现了我国法制建设的完善程度及居民素质的高低。但现行税制由于内外原因,存在各种缺陷。因此有必要在理论中找到对策,并在实践中寻求答案逐步完善个人所得税制度。     

Application of Grain Size Analysis to Study Sedimentary Environment of Sand Dune in the Vicinity of Tumen River

The dunes in estuary of Tumen River in China lie to the area between the Jiushaping and Fangchuan in the left bank of Tumen River (Fig.1). The dis-tance is about 15～20 km between the dunes and the coast. It ranges from China to D. P. R. Korea and Russia. The range of the height of dunes is about 15～20 m and the width is 100～200 m. By the re-connaissance, we protracted 9 section planes of the dunes and collected 40 sand samples of the dunes, and identified the sedimentary environment …     

Three Super Active Regions in the Descending Phase of Solar Cycle 23

We analyze the magnetic configurations of three super active regions, NOAA 10484, 10486 and 10488, observed by the Huairou Multi-Channel Solar Telescope (MCST) from 2003 October 18 to November 4. Many energetic phenomena, such as flares (including a X-28 flare) and coronal mass ejections (CMEs), occurred during this period. We think that strong shear and fast emergence of magnetic flux are the main causes of these events. The question is also of great interest why these dramatic eruptions occurred so close together in the descending phase of the solar cycle.     

应用GPS观测青藏高原东北缘应力场变化

采用各向同性弹性地球模型推导了地面位移场速率与地壳内任意点应力场变化的边界积分关系,同时利用青藏高原东北缘1999～2001年观测的GPS资料对观测区地壳深度为5 km和25 km的主应力和最大剪应力进行了计算分析.结果表明,青藏高原东北缘的主应力变化主要集中在祁连山断裂、海原断裂等,在1920～1954年间历史上发生过多次震级为7.0～8.5级强震的断裂附近,并具有主应力变化沿断层走向分布、最大剪应力沿断层走向交替变化等特征.