Use of suppressive subtractive hybridization and cDNA arrays to discover patterns of altered gene expression in the liver of dihydrotestosterone and 11-ketotestosterone exposed adult male largemouth bass (Micropterus salmoides)

In this study male largemouth bass (LMB) were exposed to the naturally occurring androgens, dihydrotestosterone (DHT) or 11-ketotestosterone (11-KT) in order to identify genes that are differentially regulated by these steroid hormones. Using subtractive hybridization on livers of fish treated with DHT against vehicle control, many novel LMB genes were cloned. These genes were added to our gene library and arrayed. Six genes were up-regulated and five were down-regulated by both androgens. But, each androgen also regulated specific genes. One gene that was identified as a potential androgen marker was spermidine-spermine-N¹-acetyltransferase that was up-regulated by both androgens. Determining which genes are responsive to natural androgens will help to identify biochemical pathways that are impacted.     

Evaluating the impact of an agricultural land‐use change adaptation strategy on household crop production in semi‐arid Ghana

In this study, the Land Use Dynamic Simulator model was applied to investigate the impact of farm credit as an adaptation strategy to cope with effects of climate variability on agricultural land‐use change and crop production in the Vea watershed in Ghana. The authors identified the determinants of crop choices within the landscape (e.g., farm household and biophysical characteristics of farm plot). The crop choice sub‐model was then linked to the crop yield sub‐model to determine the yields of selected crops. In adapting to the impacts of climate variability, the maize credit adoption sub‐model under the maize cultivation credit scenario was integrated into decision‐making. This was simulated for a 20‐year period, and compared with the business‐as‐usual scenario. Under the simulated maize credit scenario, maize adopters increased from about 20 per cent to about 50 per cent and the area allocated for maize cultivation significantly increased by about 266 per cent. Consequently, the average annual aggregated household crop yield increased by 6.3 per cent higher than in the business‐as‐usual scenario. This simulation study shows that access to maize credit can significantly influence agricultural land‐use change and food availability in the study area. However, although access to farm credit may translate into food availability, the sustainability of this strategy is questionable.     

Machine Learning Can Assign Geologic Basin to Produced Water Samples Using Major Ion Geochemistry

Natural Resources Research - Understanding the geochemistry of waters produced during petroleum extraction is essential to informing the best treatment and reuse options, which can potentially be...     

Furongian (upper Cambrian) Guole Konservat-Lagerst?tte from South China

The Guole biota contains common shelly fossils and some exceptionally well-preserved soft-bodied fossils. Stratigraphically, it is located in the mudstones and siltstones of the Sandu Formation near Guole Town, Jingxi County, Guangxi, South China. It is the first Furongian (late Cambrian) Burgess Shale-type biota found in the world, thereby filling the gap between middle Cambrian and Lower Ordovician Burgess Shale-type Lagerst?tten. Preliminary studies suggest that the Guole biota includes approximately seven metazoan groups as well as algae. These will provide important new evolutionary and ecological information.     

Origin and Formation of Sulfate in Soils from Three Ohio Counties

Sulfate induced heave has been attributed to ettringite, which can form when there is an elevated pH as well as sufficient amounts of aluminum, sulfate, calcium and water present. The primary objective of this project was to study the origin and formation of sulfate in Ohio soils in order to assist with selecting appropriate soil stabilization strategies for future roadway construction. Three roadway construction project areas were evaluated: State Route 2 in Lake (LAK) County, US Highway 24 in Paulding and Defiance Counties and Interstate-71 in Morrow (MRW) County. Defiance County had the most soil samples with sulfate concentrations above acceptable risk level (3000 mg/kg SO₄). Morrow County had the next highest number of unacceptable sulfate levels. Of the 42 Lake County soils analyzed, 11 contained sulfate above acceptable risk level. The soils surrounding the road construction activities along State Route 2 and US-24 had similar geological characteristics. A potential source of sulfate in Paulding and Defiance Counties was attributed to the direct deposition of gypsum as a soil amendment for farmlands. The most likely sources of soil sulfate in Morrow County were deposition of gypsum for farmland activities and the oxidative weathering of pyrite.     

Farming as a livelihood source for the urban poor of Nakuru, Kenya

Farming among urban dwellers in Sub-Saharan Africa is a common phenomenon. The present study, carried out in a medium-sized town in Kenya, not only confirmed this but also showed that farming by urban dwellers in the rural areas was even more important for these households’ livelihoods than farming in town. However, those who could benefit most from farming, i.e. the urban poor, appeared to be underrepresented among urban farming households and those urban poor who did farm, either in urban or in rural areas, performed worse than the farming non-poor. The importance of farming as a livelihood source is illustrated by the fact that in years with very little or no harvest due to drought, many of the poor farming households faced food shortages.     

Fractionation vs. magma mixing in the Wangrah Suite A-type granites, Lachlan Fold Belt, Australia: Experimental constraints

The Wangrah Suite granites (Lachlan Fold Belt, Australia) reflect different stages of differentiation in the magmatic history of an A-type plutonic suite. In this study we use experimentally determined phase equilibria of four natural A-type granitic compositions of the Wangrah Suite to constrain phases and phase compositions involved in fractionation processes. Each composition represents a distinct granite intrusion in the Wangrah Suite. The intrusions are the Danswell Creek (DCG), Wangrah (WG), Eastwood (EG) and Dunskeig Granite (DG), ordered from “most mafic” to “most felsic” by increasing SiO₂ and decreasing FeO_total.

Experimental investigation show that the initial water content in melts from DCG is between 2–3 wt. % H₂O. If the DCG is viewed as the parental magma for the Wangrah Suite, then (1) fractionation of magnetite, orthopyroxene and plagioclase ( 20 wt. %) of the DCG composition, leads to compositions similar to that of the EG; (2) further fractionation of plagioclase, quartz, K-feldspar and biotite ( 40 wt. %) from the EG composition, leads to the DG composition. These fractionation steps can occur nearly isobarically and are confirmed by bulk rock Ba, Sr, Rb and Zr concentrations.

In contrast, the generation of the most abundant WG composition cannot be explained by fractional crystallisation from the DCG at isobaric conditions because of the high K₂O content of this granite. Magma Mixing could be the process to explain the chemical distinctiveness of the Wangrah Granite from all the other granites of the Wangrah Suite.     

Numerical simulation of the paleohydrology of glacial Lake Oshkosh, eastern Wisconsin, USA

Proglacial lakes, formed during retreat of the Laurentide ice sheet, evolved quickly as outlets became ice-free and the earth deformed through glacial isostatic adjustment. With high-resolution digital elevation models (DEMs) and GIS methods, it is possible to reconstruct the evolution of surface hydrology. When a DEM deforms through time as predicted by our model of viscoelastic earth relaxation, the entire surface hydrologic system with its lakes, outlets, shorelines and rivers also evolves without requiring assumptions of outlet position. The method is applied to proglacial Lake Oshkosh in Wisconsin (13,600 to 12,900 cal yr BP). Comparison of predicted to observed shoreline tilt indicates the ice sheet was about 400 m thick over the Great Lakes region. During ice sheet recession, each of the five outlets are predicted to uplift more than 100 m and then subside approximately 30 m. At its maximum extent, Lake Oshkosh covered 6600 km² with a volume of 111 km³. Using the Hydrologic Engineering Center-River Analysis System model, flow velocities during glacial outburst floods up to 9 m/s and peak discharge of 140,000 m³/s are predicted, which could drain 33.5 km³ of lake water in 10 days and transport boulders up to 3 m in diameter.