Molecular identification of bloom-forming species Phaeocystis globosa (Pryninesiophyta) and its dispersal based on rDNA ITS sequence analysis

The colony-forming Phaeocystis species are causative agents of dense bloom occurrences in coastal waters worldwide. It is difficult to separate them because of the different morphologies associated with their colonial stages. In this study we applied molecular approaches to analyze the genetic variation of Phaeocystis globosa and Phaeocystis pouchetii from several geographic regions, and to assist in tracing the dispersal of bloom-forming Phaeocystis species in coastal waters of China. The sequences of the internal transcribed spacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystis strains were determined. Sequence comparison shows that P.globosa was the most divergent to P. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences existed between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearly shows that the species isolated from the southeast coast of China is identified as P.globosa rather than P. cf. pouchetii or other species. Furthermore, the significance of rDNA variation in distinct global populations of P.globosa suggested it might have had sufficient time to accumulate detectable mutations at the rDNA locus, supporting the hypothesis of ancient dispersal of P.globosa to many areas, meaning that P.globosa blooms in the coastal waters of China are endemic rather than a newly introduced species or a foreign source. Finally, based on the high divergent region of rDNA ITS, a pair of species-specific primers for P.globosa were designed, they could be useful to detect the presence of this species in mixed plankton assemblages or flagellate stages that are recognized with diffculties by means of conventional microscopy.     

利用卫星遥感技术监测赤潮的研究

赤潮灾害的频繁发生，给海洋生态环境和海洋渔业经济造成了严重危害。卫星遥感技术的不断完善，为快速、有效地动态监测赤潮灾害提供了可能，通过卫星图片的校正、合成、分析、解释、判断分析，可以迅速连续地掌握赤潮的发生状况，对于研究防控和治理赤潮灾害有着十分重要的意义，本文就如何利用卫星遥感技术监测赤潮进行了探讨。     

前苏联七十年海冰研究回顾

本文简要介绍了前苏联近七十年来海冰研究的主要成果及其采用的一些预报方法。     

Summer Arctic sea fog

I~IOXSea fog is a kind of dangerous weather. Chinese sea fog experts, Wang Binhua (1983),Hu Ruijin and Zhou Faxiu (1998) and Hu Jifu et al. (1996) studied sea fog rather Systematically. FOreign Experts also Paid great attention to sea fog. Ernlnons and Montgomery(1974), chipper (1994) and Rayrnond et al. (1989) have studied sea fog thorOUghly.HOwever, studies on Arctic sea ice have rarely been carried Out becauSe of the sever environment and less htnnan activity in the region. There …     

青岛文昌鱼的生活史──年龄、生长和死亡研究

于１９８９年８月－１９９０年７月对青岛文昌鱼的生活史进行研究。结果表明：（１）青岛文昌鱼种群由４个齿组组成，它至少生产４ａ以上，甚至可达６ａ左右。（２）在Ⅰ、Ⅱ齿时生长较快，平均年生长１３－１５ｍｍ，Ⅲ龄后生长速度减慢，即使同一年龄组的文晶鱼，在不同季节其生长速度也不同。（３）在冬春采集的文昌鱼中Ⅲ龄组的峰值较小。（４）繁殖季节过后，老龄文昌鱼因衰老而死亡。     

Molecular identification of bloom-forming species Phaeocystis globosa(Prymnesiophyta)and its dispersal based on rDNA ITS sequence analysis

Abstract-The colony-forming Phaeocystis species are causative agents of dense bloom occurrences incoastal waters worldwide. It is difficult to separate them because of the different morphologies associatedwith their colonial stages. In this study we applied molecular approaches to analyze the genetic variationof Phaeocystis globosa and Phaeocystis pouchetii from several geogrtaphic regions, and to assist in tracingthe dispersal of bloom-forming Phaeocystis species in coastal waters of China. The Sequences of the inter-nal transcribed sacers (ITS1 and ITS2) of rDNA and the 5.8S ribosomal RNA gene of Phaeocystisstrains were determined. Sequence comparison shows that P.globosa was the most divergent toP. pouchetii, exhibiting sequence divergence higher than 0.08. However, lower genetic divergences ex-isted between strains of P.globosa. The sequence comparison of the Phaeocystis rDNA ITS clearlyshows that the species isolated from the southeast coast of China is identified as P. globosa rather thanP. cf. po     

华南沿海地区全新世海平面变化特征

１海平面变化特征１．１全新世中期海侵根据地层中所含硅藻属种雷州半岛海康南田乡一１５ｍ钻孔岩芯，含有丰富的硅藻，主要属种是①：具槽直链藻（Ｍｅｌｏｓｉｒａ　ｓｕｌｃａｔａ（Ｅｈｒ）Ｋｕｔｚ）、范氏回箱藻（Ｐｙｘｉｃｌｉｃｕｌａ　ｗｅｙｐｒｅｃｈｔｉｉＧｒｕｎ）、条纹小环藻（Ｃｙｃｌｏｔｅｌｌａｓｔｒｉａｔａ（Ｋｕｔｚ）Ｇｒｕｎ）、流水双菱藻（ＳｕｒｉｒｅｌｌａｆｌｕｍｉｎｅｎｓｉｓＧｒｕｎ）。上述硅藻主要是海相生活属种，今日南田乡已距离海岸线５ｋｍ。徐闻海安西侧一１８．５ｍ钻孔岩芯中的硅藻除上述属…     

Distribution and number change of the Littorinidae (Gastropoda) on intertidal hard bed in the Jiulong River Estuary

There are 4 species of Littorinidae on the intertidal hard bed in the Jiulong River Estu-ary. They are Littorina scabra, L. brevicula, Nodilittorina pyramidalis pyramidalis and N.millegrana. Of these species, L. scabra is a dominant species. According to the results of in-vestigation, we find distribution patterns of the Littorinidae on intertidal hard bed in theestuary as follows: (1)There is no Littorinid species at Haicheng Station. (2)There is only Lit-     

湘西洛塔表层岩溶泉的生态环境类型及水文特征

岩溶石山区表层岩溶带的调蓄功能可有效控制石漠化的加剧并为严重缺水地区提供饮用水。文章以湖南洛塔为例,在大量的野外调查基础上,通过对洛塔岩溶区表层岩溶系统生态环境与表层岩溶泉水文特征的对比研究,按生态环境属性将表层岩溶泉分为三种主要类型:森林环境表层岩溶泉、石漠化环境表层岩溶泉及石漠化-森林过渡环境表层岩溶泉。详细论述上述三种表层岩溶泉的发育条件与水文特征:森林环境表层岩溶泉具有持续稳定的水文动态与流量;石漠化环境表层岩溶泉流量与降雨量相对应,动态极不稳定;石漠化与森林过渡环境表层岩溶泉流量排泄峰值具有随泉域植被覆盖率增加而缓和的趋势。按照环境类型对表层岩溶泉进行分类,可直观地显示其特殊的水文特征,有助于岩溶泉的合理开发和综合利用。     

海拉尔盆地乌尔逊凹陷与松辽盆地孤店C02气田含片钠铝石砂岩的岩石学特征

海拉尔盆地乌尔逊凹陷南屯组与松辽盆地孤店CO2气田泉头组发育大量含片钠铝石砂岩.通过偏光显微镜、扫描电镜、茜素红-S染色、X射线衍射、电子探针与INCA能谱分析等,对含片钠铝石砂岩的骨架碎屑组分、胶结物与自生矿物、成岩共生序列等岩石学特征进行了系统研究.研究表明,含片钠铝石砂岩的岩石类型为长石砂岩和岩屑长石砂岩,粒度以细粒、细-中粒为主,分选差-中等.砂岩中胶结物主要为次生加大石英、自生石英、片钠铝石、铁白云石和牯土矿物.其中,片钠铝石最高可达砂岩总体积的22%,在砂岩中或以放射状、束状、菊花状、杂乱毛发状、毛球状、板状等集合体充填孔隙,或呈束状和板状交代长石和岩屑.电子探针与INCA能谱综合分析表明,片钠铝石主要由Na、AI、O、C等组成.在含片钠铝石砂岩中,成岩共生序列依次为牯土矿物包壳-次生加大石英、自生石英、自生高岭石-油气充注-CO2充注-片钠铝石-铁白云石.其中,CO2注入前形成的自生矿物组合主要为次生加大石英、自生石英和自生高岭石,C02注入后形成的自生矿物组合主要为片钠铝石和铁白云石.